LOCUS W41085 589 bp mRNA linear EST 11-SEP-1996
DEFINITION mc41c12.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone
IMAGE:351094 5' similar to gb:J00413 Mouse beta-globin major gene
(MOUSE), mRNA sequence.
ACCESSION W41085
VERSION W41085.1
DBLINK BioSample: SAMN00155042
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 589)
AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
Waterston,R.
TITLE The WashU-HHMI Mouse EST Project
JOURNAL Unpublished
COMMENT Contact: Marra M/Mouse EST Project
WashU-HHMI Mouse EST Project
Washington University School of MedicineP
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: mouseest@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
MGI:222894
Seq primer: ETPrimer
High quality sequence stop: 424.
FEATURES Location/Qualifiers
source 1..589
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/clone="IMAGE:351094"
/clone_lib="SAMN00155042 Soares mouse p3NMF19.5"
/dev_stage="19.5 dpc total fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Vector: pT7T3D (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 5. Library constructed by Bento
Soares and M.Fatima Bonaldo. RNA was kindly provided by
Dr. Minoru Ko (Wayne State University)."
BASE COUNT 141 a 144 c 156 g 145 t
ORIGIN
1 acaaccccag aaacagacat catggtgcac ctgactgatg ctgagaaggc tgctgtctct
61 ggcctgtggg gaaaggtgaa cgccgatgaa gttggtggtg aggccctggg caggctgctg
121 gttgtctacc cttggaccca gcggtacttt gatagctttg gagacctatc ctctgcctct
181 gctatcatgg gtaatgccaa agtgaaggcc catggcaaga aagtgataac tgcctttaac
241 gatggcctga atcacttgga cagcctcaag ggcacctttg ccagcctcag tgagctccac
301 tgtgacaagc tgcatgtgga tcctgagaac ttcaggctcc tgggcaatat gatcgtgatt
361 gtgctgggcc acacctgggc aaggattcac ccccgctgca caggctgcct tccagaaggt
421 ggtggctgga gtggccgctg cctgctcaca agtacactaa acccctttcc tgctcttgca
481 tgtgaacaat ggttaattgt tcccaagaga gcatctgtna gttgttggca aaattgataa
541 gacatttgaa aatctgcgtt ctgacaaata aaaagnattt atttnactg
//