LOCUS W30652 490 bp mRNA linear EST 11-SEP-1996 DEFINITION mc14f05.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:348513 5' similar to gb:V00497 HEMOGLOBIN BETA CHAIN (HUMAN); gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence. ACCESSION W30652 VERSION W30652.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 490) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:220313 Seq primer: ETPrimer High quality sequence stop: 393. FEATURES Location/Qualifiers source 1..490 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:348513" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." BASE COUNT 106 a 127 c 142 g 115 t ORIGIN 1 caaccccaga aacagacatc atggtgcacc tgactgatgc tgagaaggct gctgtctctg 61 gcctgtgggg aaaggtgaac gccgatgaag ttggtggtga ggccctgggc aggctgctgg 121 ttgtctaccc ttggacccag cggtactttg atagctttgg agacctatcc tctgcctctg 181 ctatcatggg taatgccaaa gtgaaggccc atggcaagaa agtgataact gcctttaacg 241 atggcctgaa tcacttggac agcctcaagg gcacctttgc cagcctcagt gagctccact 301 gtgacaagct gcatgtggat cctgagaact tcaggctcct gggcaatatg atcgtgattg 361 tgctgggcca cacctgggca aggatttcac cccgctgcac aggctgcctt ccagaaggtg 421 gtggctggag tggccgctgc ctggtcacaa gtacactaaa ccgcttctct gtcttgcatg 481 tgaagatggt //