LOCUS W30456 501 bp mRNA linear EST 11-SEP-1996
DEFINITION mc20c09.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone
IMAGE:349072 5' similar to gb:J00413 Mouse beta-globin major gene
(MOUSE), mRNA sequence.
ACCESSION W30456
VERSION W30456.1
DBLINK BioSample: SAMN00155042
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 501)
AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
Waterston,R.
TITLE The WashU-HHMI Mouse EST Project
JOURNAL Unpublished
COMMENT Contact: Marra M/Mouse EST Project
WashU-HHMI Mouse EST Project
Washington University School of MedicineP
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: mouseest@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
MGI:220872
Seq primer: ETPrimer
High quality sequence stop: 433.
FEATURES Location/Qualifiers
source 1..501
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/clone="IMAGE:349072"
/clone_lib="SAMN00155042 Soares mouse p3NMF19.5"
/dev_stage="19.5 dpc total fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Vector: pT7T3D (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 5. Library constructed by Bento
Soares and M.Fatima Bonaldo. RNA was kindly provided by
Dr. Minoru Ko (Wayne State University)."
BASE COUNT 110 a 131 c 142 g 118 t
ORIGIN
1 tgactcacaa ccccagaaac agacatcatg gtgcacctga ctgatgctga gaaggctgct
61 gtctctggcc tgtggggaaa ggtgaacgcc gatgaagttg gtggtgaggc cctgggcagg
121 ctgctggttg tctacccttg gacccagcgg tactttgata gctttggaga cctatcctct
181 gcctctgcta tcatgggtaa tgccaaagtg aaggcccatg gcaagaaagt gataactgcc
241 tttaacgatg gcctgaatca cttggacagc ctcaagggca cctttgccag cctcagtgag
301 ctccactgtg acaagctgca tgtggatcct gagaacttca ggctcctggg caatatgatc
361 gtgattgtgc tgggccacca cctgggcaag gattcacccc cgctgcacag gctgccttcc
421 agaaggtggt ggctggagtg gcgctgccgt gctcacaagt acactaaacc gctttctgct
481 cttgctgtga aaatggttaa t
//