LOCUS W30452 566 bp mRNA linear EST 11-SEP-1996
DEFINITION mc21a10.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone
IMAGE:349146 5' similar to gb:V00497 HEMOGLOBIN BETA CHAIN (HUMAN);
gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence.
ACCESSION W30452
VERSION W30452.1
DBLINK BioSample: SAMN00155042
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 566)
AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
Waterston,R.
TITLE The WashU-HHMI Mouse EST Project
JOURNAL Unpublished
COMMENT Contact: Marra M/Mouse EST Project
WashU-HHMI Mouse EST Project
Washington University School of MedicineP
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: mouseest@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
MGI:220946
Putative full length read
Seq primer: ETPrimer
High quality sequence stop: 433.
FEATURES Location/Qualifiers
source 1..566
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/clone="IMAGE:349146"
/clone_lib="SAMN00155042 Soares mouse p3NMF19.5"
/dev_stage="19.5 dpc total fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Vector: pT7T3D (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 5. Library constructed by Bento
Soares and M.Fatima Bonaldo. RNA was kindly provided by
Dr. Minoru Ko (Wayne State University)."
BASE COUNT 133 a 142 c 153 g 138 t
ORIGIN
1 gttgactcac aaccccagaa acagacatca tggtgcacct gactgatgct gagaaggctg
61 ctgtctctgg cctgtgggga aaggtgaacg ccgatgaagt tggtggtgag gccctgggca
121 ggctgctggt tgtctaccct tggacccagc ggtactttga tagctttgga gacctatcct
181 ctgcctctgc tatcatgggt aatgccaaag tgaaggccca tggcaagaaa gtgataactg
241 cctttaacga tggcctgaat cacttggaca gcctcaaggg cacctttgcc agcctcagtg
301 agctccactg tgacaagctg catgtggatc ctgagaactt caggctcctg ggcaatatga
361 tcgtgattgt gctgggccac acctgggcaa ggatttcacc ccgctgcaca ggctgccttc
421 cagaaggtgg tggctggagt gcccgctgcc tggtcacaag tacactaaac ccctttcgtc
481 tcttcgtgtg aaaatggtta atgtccaaga gagactgcta ttgttgcaaa tataagcatt
541 gaaactgctc tgcaataaac gatatt
//