LOCUS W20992 839 bp mRNA linear EST 10-SEP-1996
DEFINITION mb89g11.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone
IMAGE:336644 5' similar to gb:V00497 HEMOGLOBIN BETA CHAIN (HUMAN);
gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence.
ACCESSION W20992
VERSION W20992.1
DBLINK BioSample: SAMN00155042
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 839)
AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
Waterston,R.
TITLE The WashU-HHMI Mouse EST Project
JOURNAL Unpublished
COMMENT Contact: Marra M/Mouse EST Project
WashU-HHMI Mouse EST Project
Washington University School of MedicineP
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: mouseest@watson.wustl.edu
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
MGI:218044
Seq primer: ETPrimer
High quality sequence stop: 401.
FEATURES Location/Qualifiers
source 1..839
/organism="Mus musculus"
/mol_type="mRNA"
/db_xref="taxon:10090"
/clone="IMAGE:336644"
/clone_lib="SAMN00155042 Soares mouse p3NMF19.5"
/dev_stage="19.5 dpc total fetus"
/lab_host="DH10B (ampicillin resistant)"
/note="Vector: pT7T3D (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 5. Library constructed by Bento
Soares and M.Fatima Bonaldo. RNA was kindly provided by
Dr. Minoru Ko (Wayne State University)."
BASE COUNT 192 a 187 c 240 g 219 t
ORIGIN
1 gttgtgttga ctcacaaccc cagaaacaga catcatggtg cacctgactg atgctgagaa
61 ggctgctgtc tctggcctgt ggggaaaggt gaacgccgat gaagttggtg gtgaggccct
121 gggcaggctg ctggttgtct acccttggac ccagcggtac tttgatagct ttggagacct
181 atcctctgcc tctgctatca tgggtaatgc caaagtgaag gcccatggca agaaagtgat
241 aactgccttt aacgatggcc tgaatcactt ggacagcctc aagggcacct ttgccagcct
301 cagtgagctc cactgtgaca agctgcatgt ggatcctgag aacttcaggc tcctgggcaa
361 tatgatcgtg attgtgctgg gccaccacct gggcaaggat ttcaccccgc tgcacaggct
421 gccttccaga aggtggtggc tggagtggng ctgccgtgct cacaagtaca ctagacgccg
481 ttcctgtctt gctgtgaaca tggtaattgt ccgagagaca tctgtagttg ttgcaaatgt
541 taagaattga aactgcttct caataaagat tatcactgaa gaaaaaaaaa attggcgaac
601 tttccttagg ggttattagt gcatgcgcgt taagcggctt gaacctgggt cgaatatcgt
661 gagaaccttc cgggggaatg agaccagtct tcaaatccgg aagaatgccc tatgcatcgg
721 ggttgttgca acgatttccc cggagttttt ttctttgggg cgtaaaagag gggtgttgtt
781 tttctcggca gctatggagg gtggcgggtc cgtctgttag tactaacatg tttagtgga
//