LOCUS W20962 460 bp mRNA linear EST 10-SEP-1996 DEFINITION mc16a02.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:348650 5' similar to gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence. ACCESSION W20962 VERSION W20962.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 460) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:220450 Seq primer: mob.REGA+ET High quality sequence stop: 407. FEATURES Location/Qualifiers source 1..460 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:348650" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." BASE COUNT 102 a 122 c 118 g 117 t ORIGIN 1 ttcggcacag gggctgctgg ttgtctaccc ttggacccag cggtactttg atagctttgg 61 agacctatcc tctgcctctg ctatcatggg taatgccaaa gtgaaggccc atgcanagaa 121 agtgataact gcctttaacg atggcctgaa tcacttggac agcctcaagg gcacctttgc 181 cagcctcagt gagctccact gtgacaagct gcatgtggat cctgagaact tcaggctcct 241 gggcaatatg atcgtgattg tgctgggcca cacctgggca aggatttcac ccccgctgca 301 caggctgcct tccagaaggt ggtggctgga gtggccgctg cctggctcac aagtaccact 361 aaaccccctt tcctgctctt gcctgtgaac aatggttaat tgttcccaag agagcatctg 421 tcagttgttg gcaaatgata aagacatttg aaatctgtct //