LOCUS W20950 352 bp mRNA linear EST 12-SEP-1996 DEFINITION mb96g07.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:337308 5' similar to gb:V00497 HEMOGLOBIN BETA CHAIN (HUMAN); gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence. ACCESSION W20950 VERSION W20950.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 352) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT On Sep 12, 1996 this sequence version replaced gi:1297867. Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:218708 Trace considered overall poor quality Seq primer: -28M13 rev2 from Amersham Sequence considered overall poor quality. FEATURES Location/Qualifiers source 1..352 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:337308" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." BASE COUNT 75 a 90 c 94 g 93 t ORIGIN 1 cctgcatcac gtggacagcc tcaagggcac ctttgctagc ctcagtgagc tccactgtga 61 caatctgcat gtggatcctg agaagttcag gctcctgggc aatatgatcg tgattgtgct 121 gggccaccac ctgggcaagg atttcacccc cgctgcacag gctgccttcc agaaggtggt 181 ggctggagtg gccgctgccc tggctcacta gtatcactag accccctttc ctgctcttgc 241 ctgtgaacaa tggtgaattg ttcgctagag agcatgtgtc agttgttggc gaaatgatgc 301 agacatttga aaatgtgtct tctgacagat atgaaggatt tatttcactg ca //