LOCUS W20902 499 bp mRNA linear EST 10-SEP-1996 DEFINITION mb95b09.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:337145 5' similar to gb:V00497 HEMOGLOBIN BETA CHAIN (HUMAN); gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence. ACCESSION W20902 VERSION W20902.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 499) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:218545 Seq primer: ETPrimer High quality sequence stop: 384. FEATURES Location/Qualifiers source 1..499 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:337145" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." BASE COUNT 104 a 128 c 143 g 124 t ORIGIN 1 attctgttgt gttgacttgc aacctcagaa acagacatca tggtgcacct gactgatgct 61 gagaaggctg ctgtctctgg cctgtgggga aaggtgaacg ccgatgaagt tggtggtgag 121 gccctgggca ggctgctggt tgtctaccct tggacccagc ggtactttga tagctttgga 181 gacctatcct ctgcctctgc tatcatgggt aatgccaaag tgaaggccca tggcaagaaa 241 gtgataactg cctttaacga tggcctgaat cacttggaca gcctcaaggg cacctttgcc 301 agcctcagtg agctccactg tgacaagctg catgtggatc ctgagaactt caggctcctg 361 ggcaatatga tcgtgattgt gctgggccac cacctgggca aggatttcac cccgctgcac 421 aggctgcctt ccagaaggtg gtggctggag tggctgctgc ctggctcaca agtacgacta 481 agcccttttc tgctattgc //