LOCUS W20893 448 bp mRNA linear EST 10-SEP-1996 DEFINITION mb93a07.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:336948 5' similar to gb:V00497 HEMOGLOBIN BETA CHAIN (HUMAN); gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence. ACCESSION W20893 VERSION W20893.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 448) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:218348 Seq primer: ETPrimer High quality sequence stop: 193. FEATURES Location/Qualifiers source 1..448 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:336948" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." BASE COUNT 100 a 105 c 126 g 117 t ORIGIN 1 ttctgttgtg ttgacttgca acctcagaaa cagacatcat ggtgcacctg actgatagct 61 gagaaggctg ctgtctctgg cctgtgggga aaggtgaacg ccgatgaagt tggtggtgag 121 gccctgggca ggctgctggt tgtctaccct tggacccagc ggtactttga tagctttgga 181 gacctatcct ctgcctctgc tatcatgggt aatgccagag tgaagtcata tgcatagaaa 241 gtgataactg cctttaacga tggcctgaat cactatgtac agcctcaagg gcacctttgc 301 cagcctcagt gagctccact gtgacaagct gcatgtggat ctgagaattc aggctctggg 361 caatatgatc gtgattgtac tgggctacat ctgggccagg aattcacccc gctgcacagc 421 ttgcttcaag aggttgtagc tgaatggc //