LOCUS W20857 478 bp mRNA linear EST 10-SEP-1996 DEFINITION mb93h01.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:337009 5' similar to gb:V00497 HEMOGLOBIN BETA CHAIN (HUMAN); gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence. ACCESSION W20857 VERSION W20857.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 478) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:218409 Seq primer: ETPrimer High quality sequence stop: 269. FEATURES Location/Qualifiers source 1..478 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:337009" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." BASE COUNT 104 a 126 c 136 g 112 t ORIGIN 1 cacaacccca gaaacagaca tcatggtgca cctgactgat gctgagaagg ctgctgtctc 61 tggcctgtgg ggaaaggtga acgccgatga agttggtggt gaggccctgg gcaggctgct 121 ggttgtctac ccttggaccc agcggtactt tgatagcttt ggagacctat cctctgcctc 181 tgctatcatg ggtaatgcca aagtgaaggc ccatggcaag aaagtgataa ctgcctttaa 241 cgatggcctg aatcacttgg acagcctcaa gggcaccttt gccagcctca gtgagctcca 301 ctgtgacaag ctgcatgtgg atcctgagaa cttcaggctc ctgggcaata tgatcgtgat 361 tgtgctgggc cacacctggg caaggatttc accccgctgc acaggctgct tccagaaggt 421 ggtggctgga gattgcgctg ccctggctaa agtacactaa acccctttcg tgtcttgc //