LOCUS W20708 476 bp mRNA linear EST 12-SEP-1996 DEFINITION mb93e09.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:337000 5' similar to gb:U01317_cds4 HEMOGLOBIN DELTA CHAIN (HUMAN); gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence. ACCESSION W20708 VERSION W20708.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 476) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT On Sep 12, 1996 this sequence version replaced gi:1297590. Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:218400 Trace considered overall poor quality Seq primer: -28M13 rev2 from Amersham Sequence considered overall poor quality. FEATURES Location/Qualifiers source 1..476 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:337000" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." BASE COUNT 113 a 119 c 129 g 115 t ORIGIN 1 cacaaatcca gatacagaca tcatggtgca cctgactgat gctgagaagg ctgctgtctc 61 tggcctgtgg gcaaacgtga acgccgatga agttggtggt gaggccctgg gcaagctact 121 ggttgtctac acttggaccc agcggtactt tgatagcttt ggagacctat cctctgcctc 181 tgctatcatg agtaatgcta aagtgaaggc ccatggcaag aaagtgataa ctgcctctaa 241 cgatatcctg aatcacttgg acatgctcaa ggcaccttag ccagactcag tgagctcgac 301 tgtgacaagc tgcatgtgga tcctgagaac ttcaggctcc tgggcaatat gatcgtgatt 361 gtgctgggcg acacctgggc aaggatttca tcccgctgca caggctgact gcgagaaggt 421 tggtagctga gtggcgctag cctgctcaaa gtacactaaa ccgctttcct gctctt //