LOCUS       W20708                   476 bp    mRNA    linear   EST 12-SEP-1996
DEFINITION  mb93e09.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone
            IMAGE:337000 5' similar to gb:U01317_cds4 HEMOGLOBIN DELTA CHAIN
            (HUMAN); gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA
            sequence.
ACCESSION   W20708
VERSION     W20708.1
DBLINK      BioSample: SAMN00155042
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     On Sep 12, 1996 this sequence version replaced gi:1297590.
            Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:218400
            Trace considered overall poor quality
            Seq primer: -28M13 rev2 from Amersham
            Sequence considered overall poor quality.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:337000"
                     /clone_lib="SAMN00155042 Soares mouse p3NMF19.5"
                     /dev_stage="19.5 dpc total fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. RNA was kindly provided by
                     Dr. Minoru Ko (Wayne State University)."
BASE COUNT          113 a          119 c          129 g          115 t
ORIGIN      
        1 cacaaatcca gatacagaca tcatggtgca cctgactgat gctgagaagg ctgctgtctc
       61 tggcctgtgg gcaaacgtga acgccgatga agttggtggt gaggccctgg gcaagctact
      121 ggttgtctac acttggaccc agcggtactt tgatagcttt ggagacctat cctctgcctc
      181 tgctatcatg agtaatgcta aagtgaaggc ccatggcaag aaagtgataa ctgcctctaa
      241 cgatatcctg aatcacttgg acatgctcaa ggcaccttag ccagactcag tgagctcgac
      301 tgtgacaagc tgcatgtgga tcctgagaac ttcaggctcc tgggcaatat gatcgtgatt
      361 gtgctgggcg acacctgggc aaggatttca tcccgctgca caggctgact gcgagaaggt
      421 tggtagctga gtggcgctag cctgctcaaa gtacactaaa ccgctttcct gctctt
//