LOCUS W20540 417 bp mRNA linear EST 10-SEP-1996 DEFINITION mb92c08.r1 Soares mouse p3NMF19.5 Mus musculus cDNA clone IMAGE:336878 5' similar to gb:V00497 HEMOGLOBIN BETA CHAIN (HUMAN); gb:J00413 Mouse beta-globin major gene (MOUSE), mRNA sequence. ACCESSION W20540 VERSION W20540.1 DBLINK BioSample: SAMN00155042 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 417) AUTHORS Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T., Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M., Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B., Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and Waterston,R. TITLE The WashU-HHMI Mouse EST Project JOURNAL Unpublished COMMENT Contact: Marra M/Mouse EST Project WashU-HHMI Mouse EST Project Washington University School of MedicineP 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: mouseest@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. MGI:218278 Seq primer: ETPrimer High quality sequence stop: 275. FEATURES Location/Qualifiers source 1..417 /organism="Mus musculus" /mol_type="mRNA" /db_xref="taxon:10090" /clone="IMAGE:336878" /clone_lib="SAMN00155042 Soares mouse p3NMF19.5" /dev_stage="19.5 dpc total fetus" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA was kindly provided by Dr. Minoru Ko (Wayne State University)." BASE COUNT 87 a 107 c 123 g 100 t ORIGIN 1 ggtgaacgcc gatgaagttg gtggtgaggc cctgggcagg ctgctggttg tctacccttg 61 gacccagcgg tactttgata gcgttggaga cctatcctct gcctctgcta tcatgggtaa 121 tgccaaagtg aaggcccatg gcaagaaagt gataactgcc tttaacgatg gcctgaatca 181 cttggacagc ctcaagggca cctttgccag cctcagtgag ctccactgtg acaagctgca 241 tgtggatcct gagaacttca ggctcctggg caatatgatc gtgattgtgc tgggccacac 301 ctgggcaagg atttgacccc gctgcacagg ctgccttcca gaaggtggtg gctggagtgg 361 cgctgccatg gtcacaagta ccactaaacc gctttgctct cttggtgtga caatggt //