LOCUS W03476 528 bp mRNA linear EST 19-APR-1996 DEFINITION za08b09.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:291929 5', mRNA sequence. ACCESSION W03476 VERSION W03476.1 DBLINK BioSample: SAMN00154979 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 528) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: ETPrimer High quality sequence stop: 486. FEATURES Location/Qualifiers source 1..528 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:3885059" /db_xref="taxon:9606" /clone="IMAGE:291929" /sex="male" /tissue_type="melanocyte" /clone_lib="SAMN00154979 Soares melanocyte 2NbHM" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from normal foreskin melanocytes (FS374) was kindly provided by Dr. Anthony P. Albino." BASE COUNT 134 a 132 c 113 g 142 t ORIGIN 1 cagccactga ggacaaccaa gtttggaaac agagccccca gctagagatc agagtgcagg 61 gtgcttccag ctctgctgca cctcccacat tgaatccagc tcctcagaaa tcagctgctc 121 caggaactgc tcctgaggag gcccctggct ctgcctccgc cgccaacccc atcttctgag 181 gatccaggct tttcttctcc tctggggatg ccagatcctc atctgtatca ccagatgggc 241 cttcttctca aacacatgca ggatgtgaga gtcctcctcg gtcacctgct catggagttg 301 agggaattat ctggccaccg gaagcctggg accacaaagg ctactgctga atagaagtaa 361 acagttcatc atgantcntt aaccacccca ataaantgat ctttatttct ctnctgtctg 421 ncatatncat aagtatttta caagttgtcc agtgttttgt tagaataatg tagttaggtg 481 agtgtaataa atttatataa agtgagatta gagttantat aattgtgt //