LOCUS       W03476                   528 bp    mRNA    linear   EST 19-APR-1996
DEFINITION  za08b09.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone
            IMAGE:291929 5', mRNA sequence.
ACCESSION   W03476
VERSION     W03476.1
DBLINK      BioSample: SAMN00154979
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Seq primer: ETPrimer
            High quality sequence stop: 486.
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3885059"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:291929"
                     /sex="male"
                     /tissue_type="melanocyte"
                     /clone_lib="SAMN00154979 Soares melanocyte 2NbHM"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library constructed by Bento Soares and
                     M.Fatima Bonaldo. RNA from normal foreskin melanocytes
                     (FS374) was kindly provided by Dr. Anthony P. Albino."
BASE COUNT          134 a          132 c          113 g          142 t
ORIGIN      
        1 cagccactga ggacaaccaa gtttggaaac agagccccca gctagagatc agagtgcagg
       61 gtgcttccag ctctgctgca cctcccacat tgaatccagc tcctcagaaa tcagctgctc
      121 caggaactgc tcctgaggag gcccctggct ctgcctccgc cgccaacccc atcttctgag
      181 gatccaggct tttcttctcc tctggggatg ccagatcctc atctgtatca ccagatgggc
      241 cttcttctca aacacatgca ggatgtgaga gtcctcctcg gtcacctgct catggagttg
      301 agggaattat ctggccaccg gaagcctggg accacaaagg ctactgctga atagaagtaa
      361 acagttcatc atgantcntt aaccacccca ataaantgat ctttatttct ctnctgtctg
      421 ncatatncat aagtatttta caagttgtcc agtgttttgt tagaataatg tagttaggtg
      481 agtgtaataa atttatataa agtgagatta gagttantat aattgtgt
//