LOCUS       W01447                   316 bp    mRNA    linear   EST 18-APR-1996
DEFINITION  za73h03.r1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone
            IMAGE:298229 5' similar to gb:M11353 HISTONE H3.3 (HUMAN), mRNA
            sequence.
ACCESSION   W01447
VERSION     W01447.1
DBLINK      BioSample: SAMN00155027
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Putative full length read
            Seq primer: mob.REGA+ET
            High quality sequence stop: 263.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:1243151"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:298229"
                     /clone_lib="SAMN00155027 Soares_fetal_lung_NbHL19W"
                     /dev_stage="19 weeks"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: lung; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTT-3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 5. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. This library was constructed
                     from the same fetus as the fetal heart library, Soares
                     fetal heart NbHH19W."
BASE COUNT           83 a           77 c           75 g           79 t
ORIGIN      
        1 agttcggcat gaggggcgct ccgtgaaatt agacgttatc agaagtccac tgaacttctg
       61 attcgcaaac ttcccttcca gcgtctggtg cgagaaattg ctcaggactt taaaacagat
      121 ctgcgcttcc agagcnacta ctccggtgct ttgcaggagg caagtgaggc ctatctggtt
      181 ggcctttttg aagacaccaa cctgtgtgct atccatgcca aacgtgtaac aattatgcca
      241 aaagacatcc agctagcacg ccggcatacg tggagaacgt gcttaagaat ccactatgat
      301 gggnaacatt tcattt
//