LOCUS W01447 316 bp mRNA linear EST 18-APR-1996 DEFINITION za73h03.r1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone IMAGE:298229 5' similar to gb:M11353 HISTONE H3.3 (HUMAN), mRNA sequence. ACCESSION W01447 VERSION W01447.1 DBLINK BioSample: SAMN00155027 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 316) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Putative full length read Seq primer: mob.REGA+ET High quality sequence stop: 263. FEATURES Location/Qualifiers source 1..316 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:1243151" /db_xref="taxon:9606" /clone="IMAGE:298229" /clone_lib="SAMN00155027 Soares_fetal_lung_NbHL19W" /dev_stage="19 weeks" /lab_host="DH10B (ampicillin resistant)" /note="Organ: lung; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTT-3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. This library was constructed from the same fetus as the fetal heart library, Soares fetal heart NbHH19W." BASE COUNT 83 a 77 c 75 g 79 t ORIGIN 1 agttcggcat gaggggcgct ccgtgaaatt agacgttatc agaagtccac tgaacttctg 61 attcgcaaac ttcccttcca gcgtctggtg cgagaaattg ctcaggactt taaaacagat 121 ctgcgcttcc agagcnacta ctccggtgct ttgcaggagg caagtgaggc ctatctggtt 181 ggcctttttg aagacaccaa cctgtgtgct atccatgcca aacgtgtaac aattatgcca 241 aaagacatcc agctagcacg ccggcatacg tggagaacgt gcttaagaat ccactatgat 301 gggnaacatt tcattt //