LOCUS W01104 430 bp mRNA linear EST 18-APR-1996 DEFINITION yz94e09.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:290728 5' similar to SW:RL3_HUMAN P39023 60S RIBOSOMAL PROTEIN L3. [1], mRNA sequence. ACCESSION W01104 VERSION W01104.1 DBLINK BioSample: SAMN00154979 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 430) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: ETPrimer High quality sequence stop: 388. FEATURES Location/Qualifiers source 1..430 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:3883858" /db_xref="taxon:9606" /clone="IMAGE:290728" /sex="male" /tissue_type="melanocyte" /clone_lib="SAMN00154979 Soares melanocyte 2NbHM" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from normal foreskin melanocytes (FS374) was kindly provided by Dr. Anthony P. Albino." BASE COUNT 109 a 104 c 129 g 86 t ORIGIN 1 ggcgtgatgt ctcacagaaa gttctccgct cccagacatg ggtccctcgg cttcctgcct 61 cggaagcgca cagcaggcat cgtgggaagg tgaagagctt ccctaaggat gacccgtcca 121 agccggtcca cctcacagcc ttcctgggat acaaggctgg catgactcac atcgtgcggg 181 aagtcgacag gccgggatcc aaggtgaaca agaaggaggt ggtggaggct gtgaccattg 241 tagagacacc acccatggtg ttgtgggcat tgtgggctac gtggaaaccc ctcgaggcct 301 ccggaccttc aagactgtct ttgctgagca catcagtgat gantgcaaga ggcgtttcta 361 taagaattgg cataaatcta agaagaaggc tttacaagta ctgcaagaaa tggcaggatg 421 aggatgnaag //