LOCUS SCU40398 5066 bp DNA linear SYN 13-MAR-1997
DEFINITION Synthetic construct hygromycin B phosphotransferase (hyg) gene, and
ampicillin resistance gene, complete cds.
ACCESSION U40398
VERSION U40398.1
KEYWORDS .
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 5066)
AUTHORS Yin,D.X. and Schimke,R.T.
TITLE BCL-2 expression delays drug-induced apoptosis but does not
increase clonogenic survival after drug treatment in HeLa cells
JOURNAL Cancer Res. 55 (21), 4922-4928 (1995)
PUBMED 7585531
REFERENCE 2 (bases 1 to 5066)
AUTHORS Yin,D.X., Zhu,L., Zahl,N. and Zhou,H.
TITLE Direct Submission
JOURNAL Submitted (06-NOV-1995) Dong X. Yin, CLONTECH Laboratories, 1020 E.
Meadow Circle, Palo Alto, CA 94303, USA
COMMENT This plasmid will be available from CLONTECH January 1996. Inquire
at 1-800-662-clon.
FEATURES Location/Qualifiers
source 1..5066
/organism="synthetic construct"
/mol_type="genomic DNA"
/db_xref="taxon:32630"
gene complement(1540..2577)
/gene="hyg"
CDS complement(1540..2577)
/gene="hyg"
/note="driven by HSV tk promoter; confers hygromycin
resistance; An out-of-frame translation intiation codon
has been removed from the hygromycin resistance gene so
that the translation efficiency is improved by
approximately 5 fold. Because this is an efficiently
translated hygromycin resistance gene driven by an
enhancer-less promoter, it is the selection marker of
choice to be used with many inducible gene expression
systems, especially the tetracyline-controlled gene
expression systems"
/codon_start=1
/transl_table=11
/product="hygromycin B phosphotransferase"
/protein_id="AAC53682.1"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRGDREMGEAN"
regulatory complement(1562..2050)
/regulatory_class="polyA_signal_sequence"
/gene="hyg"
/note="HSV tk polyA signal"
regulatory complement(2691..2839)
/regulatory_class="promoter"
/note="HSV tk promoter"
rep_origin 3182..3825
/note="pBR322 replication origin"
gene complement(3973..4833)
/gene="ampicillin resistance gene"
CDS complement(3973..4833)
/gene="ampicillin resistance gene"
/codon_start=1
/transl_table=11
/protein_id="AAC53683.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1226 a 1368 c 1246 g 1226 t
ORIGIN
1 ctggcgtaat agcgaagagg cccgcaccga tccagacatg ataagataca ttgatgagtt
61 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
121 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
181 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
241 ctacaaatgt ggtatggctg attatgatcc ccaggaagct cctctgtgtc ctcataaacc
301 ctaacctcct ctacttgaga ggacattcca atcataggct gcccatccac cctctgtgtc
361 ctcctgttaa ttaggtcact taacaaaaag gaaattgggt aggggttttt cacagaccgc
421 tttctaaggg taattttaaa atatctggga agtcccttcc actgctgtgt tccagaagtg
481 ttggtaaaca gcccacaaat gtcaacagca gaaacataca agctgtcagc tttgcacaag
541 ggcccaacac cctgctcatc aagaagcact gtggttgctg tgttagtaat gtgcaaaaca
601 ggaggcacat tttccccacc tgtgtaggtt ccaaaatatc tagtgttttc atttttactt
661 ggatcaggaa cccagcactc cactggataa gcattatcct tatccaaaac agccttgtgg
721 tcagtgttca tctgctgact gtcaactgta gcattttttg gggttacagt ttgagcagga
781 tatttggtcc tgtagtttgc taacacaccc tgcagctcca aaggttcccc accaacagca
841 aaaaaatgaa aatttgaccc ttgaatgggt tttccagcac cattttcatg agttttttgt
901 gtccctgaat gcaagtttaa catagcagtt accccaataa cctcagtttt aacagtaaca
961 gcttcccaca tcaaaatatt tccacaggtt aagtcctcat ttaaattagg caaaggaatt
1021 cctcgacctg cagcccaagc ttggcactgg cgccagaaat ccgcgcggtg gtttttgggg
1081 gtcgggggtg tttggcagcc acagacgccc ggtgttcgtg tcgcgccagt acatgcggtc
1141 catgcccagg ccatccaaaa accatgggtc tgtctgctca gtccagtcgt ggacctgacc
1201 ccacgcaacg cccaaaagaa taacccccac gaaccataaa ccattcccca tgggggaccc
1261 cgtccctaac ccacggggcc cgtggctatg gcgggcttgc cgccccgacg ttggctgcga
1321 gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa acgcgggcgt
1381 attggcccca atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc
1441 cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt tattgccgtc
1501 atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct cccccatctc
1561 ccgatcccca cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
1621 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
1681 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
1741 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
1801 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
1861 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
1921 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
1981 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
2041 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
2101 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
2161 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
2221 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
2281 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
2341 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
2401 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
2461 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
2521 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
2581 aagctgatct tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg gtcgctcggt
2641 gttcgaggcc acacgcgtca ccttaatatg cgaagtggac ctgggaccgc gccgccccga
2701 ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt gtgtcatcat
2761 agaactaaag acatgcaaat atatttcttc cggggacacc gccagcaaac gcgagcaacg
2821 ggccacgggg atgaagcagc ccggcggcac ctcgctaacg gattcaccac tccaagaatt
2881 ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata
2941 tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcggggcc gacgcgctgg
3001 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
3061 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
3121 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagcgcc agcaaaaggc
3181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
3241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
3301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
3361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
3421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
3481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
3541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
3601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
3661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
3721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
3781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
3841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
3901 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
3961 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
4021 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
4081 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
4141 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
4201 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
4261 tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg
4321 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
4381 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
4441 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
4501 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
4561 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
4621 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
4681 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
4741 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
4801 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
4861 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
4921 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
4981 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
5041 attctcatgt ttgacagctt atcatc
//