LOCUS HSU21247 11954 bp RNA linear VRL 26-JUL-2016
DEFINITION Human spumaretrovirus complete genome.
ACCESSION U21247
VERSION U21247.1
KEYWORDS gag protein; long terminal repeat; pol polyprotein.
SOURCE Human spumaretrovirus
ORGANISM Human spumaretrovirus
Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes;
Ortervirales; Retroviridae; Spumaretrovirinae; Spumavirus; Simian
foamy virus.
REFERENCE 1 (bases 9435 to 10337)
AUTHORS Flugel,R.M., Rethwilm,A., Maurer,B. and Darai,G.
TITLE Nucleotide sequence analysis of the env gene and its flanking
regions of the human spumaretrovirus reveals two novel genes
JOURNAL EMBO J. 6 (7), 2077-2084 (1987)
PUBMED 2820721
REMARK Erratum:[EMBO J 1990 Nov;9(11):3806]
REFERENCE 2 (bases 5337 to 11954)
AUTHORS Maurer,B., Bannert,H., Rethwilm,A., Darai,G. and Fluegel,R.M.
TITLE Characterization of the ENV gene and of two novel coding regions of
the human spumaretrovirus
JOURNAL (in) Bolognesi,D.P. (Ed.);
HUMAN RETROVIRUSES, CANCER AND AIDS: 75-88;
Alan Liss, Inc., New York (1988)
REFERENCE 3 (bases 1 to 5342)
AUTHORS Maurer,B., Bannert,H., Darai,G. and Flugel,R.M.
TITLE Analysis of the primary structure of the long terminal repeat and
the gag and pol genes of the human spumaretrovirus
JOURNAL J. Virol. 62 (5), 1590-1597 (1988)
PUBMED 2451755
REFERENCE 4 (bases 1 to 11954)
AUTHORS Muranyi,W. and Flugel,R.M.
TITLE Analysis of splicing patterns of human spumaretrovirus by
polymerase chain reaction reveals complex RNA structures
JOURNAL J. Virol. 65 (2), 727-735 (1991)
PUBMED 1846194
REFERENCE 5 (bases 1 to 11954)
AUTHORS Lochelt,M., Zentgraf,H. and Flugel,R.M.
TITLE Construction of an infectious DNA clone of the full-length human
spumaretrovirus genome and mutagenesis of the bel 1 gene
JOURNAL Virology 184 (1), 43-54 (1991)
PUBMED 1651600
REFERENCE 6 (bases 1 to 11954)
AUTHORS Lochelt,M., Muranyi,W. and Flugel,R.M.
TITLE Human foamy virus genome possesses an internal, Bel-1-dependent and
functional promoter
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 90 (15), 7317-7321 (1993)
PUBMED 8394017
REFERENCE 7 (bases 1 to 11954)
AUTHORS Weissenberger,J. and Flugel,R.M.
TITLE Identification and characterization of the Bel 3 protein of human
foamy virus
JOURNAL AIDS Res. Hum. Retroviruses 10 (5), 595-600 (1994)
PUBMED 7917520
REFERENCE 8 (bases 1 to 11954)
AUTHORS Kogel,D., Aboud,M. and Flugel,R.M.
TITLE Mutational analysis of the reverse transcriptase and ribonuclease H
domains of the human foamy virus
JOURNAL Nucleic Acids Res. 23 (14), 2621-2625 (1995)
PUBMED 7544460
REFERENCE 9 (bases 1 to 11954)
AUTHORS Lochelt,M. and Flugel,R.M.
TITLE The human foamy virus pol gene is expressed as a Pro-Pol
polyprotein and not as a Gag-Pol fusion protein
JOURNAL J. Virol. 70 (2), 1033-1040 (1996)
PUBMED 8551561
REFERENCE 10 (bases 1 to 11954)
AUTHORS Bodem,J., Lochelt,M., Winkler,I., Flower,R.P., Delius,H. and
Flugel,R.M.
TITLE Characterization of the spliced pol transcript of feline foamy
virus: the splice acceptor site of the pol transcript is located in
gag of foamy viruses
JOURNAL J. Virol. 70 (12), 9024-9027 (1996)
PUBMED 8971036
REMARK Erratum:[J Virol 1997 Nov;71(11):8952]
REFERENCE 11 (bases 1 to 11954)
AUTHORS Fluegel,R.M.
TITLE Direct Submission
JOURNAL Submitted (21-FEB-1995) Rolf M. Fluegel, Abtlg. Retroviral Gene
Expression, DKFZ-ATZ, Heidelberg 69009, Germany
FEATURES Location/Qualifiers
source 1..11954
/organism="Human spumaretrovirus"
/mol_type="genomic RNA"
/db_xref="taxon:11963"
/clone="pHSRV13, infectious"
repeat_region 1..1121
/note="5' long terminal repeat"
/rpt_type=long_terminal_repeat
repeat_region 1..11
/rpt_type=inverted
protein_bind 265..271
/bound_moiety="AP-1"
protein_bind 320..326
/bound_moiety="AP-1"
regulatory 749..754
/regulatory_class="TATA_box"
prim_transcript 778..11800
/note="gag gene"
/citation=[4]
/citation=[9]
/citation=[10]
prim_transcript join(778..829,829..6451,6493..11800)
/note="secondary transcript"
/citation=[4]
/citation=[9]
/citation=[10]
misc_feature 778
/note="start site of transcription; gag-pol-env genes"
repeat_region 780..968
/note="LTR R-region"
misc_feature 829
/note="major 5' splice donor site for all subgenomic
transcripts including pol, env, bel, and bet genes"
repeat_region 1111..1121
/note="3' LTR"
/rpt_type=inverted
misc_binding 1124..1141
/bound_moiety="Lys-tRNA 1 and 2 primer"
gene 1223..3169
/gene="gag"
CDS 1223..3169
/gene="gag"
/note="matrix protein; 78 kDa form"
/codon_start=1
/product="gag protein precursor"
/protein_id="AAB48111.1"
/translation="MASGSNVEEYELDVEALVVILRDRNIPRNPLHGEVIGLRLTEGW
WGQIERFQMVRLILQDDDNEPLQRPRYEVIQRAVNPHTMFMISGPLAELQLAFQDLDL
PEGPLRFGPLANGHYVQGDPYSSSYRPVTMAETAQMTRDELEDVLNTQSEIEIQMINL
LELYEVETRALRRQLAERSSTGQGGISPGAPRSRPPVSSFSGLPSLPSIPGIHPRAPS
PPRATSTPGNIPWSLGDDSPPSSSFPGPSQPRVSFHPGNPFVEEEGHRPRSQSRERRR
EILPAPVPSAPPMIQYIPVPPPPPIGTVIPIQHIRSVTGEPPRNPREIPIWLGRNAPA
IDGVFPVTTPDLRCRIINAILGGNIGLSLTPGDCLTWDSAVATLFIRTHGTFPMHQLG
NVIKGIVDQEGVATAYTLGMMLSGQNYQLVSGIIRGYLPGQAVVTALQQRLDQEIDNQ
TRAETFIQHLNAVYEILGLNARGQSIRASVTPQPRPSRGRGRGQNTSRPSQGPANSGR
GRQRPASGQSNRGSSTQNQNQDNLNQGGYNLRPRTYQPQRYGGGRGRRWNDNTNNQES
RPSDQGSQTPRPNQAGSGVRGNQSQTPRPAAGRGGRGNHNRNQRSSGAGDSRAVNTVT
QSATSSTDESSSAVTAASGGDQRD"
mat_peptide 1832..3166
/gene="gag"
/product="gag protein"
/inference="non-experimental evidence, no additional
details recorded"
/note="74 kDa form"
misc_feature 1223..1831
/gene="gag"
/note="encodes matrix protein of the gag protein"
misc_feature <1616..3166
/gene="gag"
/note="encodes gag precursor, 74 kDa form including matrix
protein (MA); capsid antigen (CA); nucleocapsid protein
(NC), putative 3 kDa"
CDS <3117..6548
/citation=[10]
/codon_start=1
/product="pro-pol protein"
/protein_id="AAB48112.1"
/translation="MNPLQLLQPLPAEIKGTKLLAHWDSGATITCIPESFLEDEQPIK
KTLIKTIHGEKQQNVYYVTFKVKGRKVEAEVIASPYEYILLSPTDVPWLTQQPLQLTI
LVPLQEYQEKILSKTALPEDQKQQLKTLFVKYDNLWQHWENQVGHRKIRPHNIATGDY
PPRPQKQYPINPKAKPSIQIVIDDLLKQGVLTPQNSTMNTPVYPVPKPDGRWRMVLDY
REVNKTIPLTAAQNQHSAGILATIVRQKYKTTLDLANGFWAHPITPESYWLTAFTWQG
KQYCWTRLPQGFLNSPALFTADVVDLLKEIPNVQVYVDDIYLSHDDPKEHVQQLEKVF
QILLQAGYVVSLKKSEIGQKTVEFLGFNITKEGRGLTDTFKTKLLNITPPKDLKQLQS
ILGLLNFARNFIPNFAELVQPLYNLIASAKGKYIEWSEENTKQLNMVIEALNTASNLE
ERLPEQRLVIKVNTSPSAGYVRYYNETGKKPIMYLNYVFSKAELKFSMLEKLLTTMHK
ALIKAMDLAMGQEILVYSPIVSMTKIQKTPLPERKALPIRWITWMTYLEDPRIQFHYD
KTLPELKHIPDVYTSSQSPVKHPSQYEGVFYTDGSAIKSPDPTKSNNAGMGIVHATYK
PEYQVLNQWSIPLGNHTAQMAEIAAVEFACKKALKIPGPVLVITDSFYVAESANKELP
YWKSNGFVNNKKKPLKHISKWKSIAECLSMKPDITIQHEKGISLQIPVFILKGNALAD
KLATQGSYVVNCNTKKPNLDAELDQLLQGHYIKGYPKQYTYFLEDGKVKVSRPEGVKI
IPPQSDRQKIVLQAHNLAHTGREATLLKIANLYWWPNMRKDVVKQLGRCQQCLITNAS
NKASGPILRPDRPQKPFDKFFIDYIGPLPPSQGYLYVLVVVDGMTGFTWLYPTKAPST
SATVKSLNVLTSIAIPKVIHSDQGAAFTSSTFAEWAKERGIHLEFSTPYHPQSGSKVE
RKNSDIKRLLTKLLVGRPTKWYDLLPVVQLALNNTYSPVLKYTPHQLLFGIDSNTPFA
NQDTLDLTREEELSLLQEIRTSLYHPSTPPASSRSWSPVVGQLVQERVARPASLRPRW
HKPSTVLKVLNPRTVVILDHLGNNRTVSIDNLKPTSHQNGTTNDTATMDHLEKNE"
misc_difference 3150
/gene="gag"
/note="conflict; sequence derived from individual clones
that are in conflict with infectious clone pHSRV13"
/citation=[3]
/replace=""
misc_difference 3153..3154
/gene="gag"
/note="conflict"
/citation=[3]
/replace=""
misc_feature <5453..6548
/note="encodes integrase; produced by proteolytic cleavage
from the putative 125 kDa pro-pol protein"
misc_feature 5453..5456
/note="encodes putative cleavage site of integrase
protein"
CDS 6496..9465
/citation=[1]
/codon_start=1
/product="envelope protein"
/protein_id="AAB48113.1"
/translation="MAPPMTLQQWIIWKKMNKAHEALQNTTTVTEQQKEQIILDIQNE
EVQPTRRDKFRYLLYTCCATSSRVLAWMFLVCILLIIVLVSCFVTISRIQWNKDIQVL
GPVIDWNVTQRAVYQPLQTRRIARSLRMQHPVPKYVEVNMTSIPQGVYYEPHPEPIVV
KERVLGLSQILMINSENIANNANLTQEVKKLLTEMVNEEMQSLSDVMIDFEIPLGDPR
DQEQYIHRKCYQEFANCYLVKYKEPKPWPKEGLIADQCPLPGYHAGLTYNRQSIWDYY
IKVESIRPANWTTKSKYGQARLGSFYIPSSLRQINVSHVLFCSDQLYSKWYNIENTIE
QNERFLLNKLNNLTSGTSVLKKRALPKDWSSQGKNALFREINVLDICSKPESVILLNT
SYYSFSLWEGDCNFTKDMISQLVPECDGFYNNSKWMHMHPYACRFWRSKKNEKEETKC
RDGETKRCLYYPLWDSPESTYDFGYLAYQKNFPSPICIEQQKIRDQDYEVYSLYQERK
IASKAYGIDTVLFSLKNFLNYTGTPVNEMPNARAFVGLIDPKFPPSYPNVTREHYTSC
NNRKRRSVDNNYAKLRSMGYALTGAVQTLSQISDINDENLQQGIYLLRDHVITLMEAT
LHDISVMEGMFAVQHLHTHLNHLKTMLLERRIDWTYMSSTWLQQQLQKSDDEMKVIKR
IARSLVYYVKQTHSSPTATAWEIGLYYELVIPKHIYLNNWNVVNIGHLVKSAGQLTHV
TIAHPYEIINKECVETIYLHLEDCTRQDYVICDVVKIVQPCGNSSDTSDCPVWAEAVK
EPFVQVNPLKNGSYLVLASSTDCQIPPYVPSIVTVNETTSCFGLDFKRPLVAEERLSF
EPRLPNLQLRLPHLVGIIAKIKGIKIEVTSSGESIKEQIERAKAELLRLDIHEGDTPA
WIQQLAAATKDVWPAAASALQGIGNFLSGTAQGIFGTAFSLLGYLKPILIGVGVILLV
ILIFKIVSWIPTKKKNQ"
regulatory 9019..9195
/regulatory_class="promoter"
/note="internal promoter for bel1, bel2 and bet genes"
/citation=[6]
gene 9165..9169
/gene="bel"
regulatory 9165..9169
/regulatory_class="TATA_box"
/gene="bel"
/citation=[6]
misc_feature 9196..9309
/note="leader RNA formed from the internal promoter for
bel1, bel2 and Bet genes"
misc_feature 9196
/note="cap site"
gene 9435..11184
/gene="bet"
CDS join(9435..9699,10001..11184)
/gene="bet"
/citation=[5]
/codon_start=1
/product="Bet"
/protein_id="AAB48114.1"
/translation="MDSYEKEESVASTSGIQDLQTLSELVGPENAGEGELTIAEEPEE
NPRRPRRYTKREVKCVSYHAYKEIEDKHPQHIKLQDWIPTPEEMIAQKVQNQDLGTIL
SFDVTCLKSITSLGRNDPGDDPSIMSHVLPVVTPWPMSQDHYAPTLFGILDRYYQGYL
KSPATYQTWKFTCQVDPSGKRFMETQFWVPPLGQVNIQFYKNYQILTCCQAVDPFANI
FHGTDEEMFDIDSGPDVWCSPSLCFKVIYEGAMGQKQEQKTWLCRLGHGHRMGACDYR
KVDLYAMRQGKENPYGDRGDAALQYAYQVKRGCKAGCLASPVLNYKALQFHRTIMADF
TNPRIGEGHLAHGYQAAMEAYGPQRGSNEERVWWNVTRNQGKQGGEYYREGGEEPHYP
NTPAPHRRTWDERHKVLKLSSFATPSDIQRWTTKALPYGWKVVTESGNDYTSRRKIRT
LTEMTQDEIRKRWESGYCDPFIDSGSDSDGPF"
gene 9435..10337
/gene="bel1"
CDS 9435..10337
/gene="bel1"
/note="transcriptional transactivator"
/codon_start=1
/product="Bel1"
/protein_id="AAB48115.1"
/translation="MDSYEKEESVASTSGIQDLQTLSELVGPENAGEGELTIAEEPEE
NPRRPRRYTKREVKCVSYHAYKEIEDKHPQHIKLQDWIPTPEEMSKSLCKRLILCGLY
SAEKASEILRMPFTVSWEQSDTDPDCFIVSYTCIFCDAVIHDPMPIRWDPEVGIWVKY
KPLRGIVGSAVFIMHKHQRNCSLVKPSTSCSEGPKPRPRHDPVLRCDMFEKHHKPRQK
RPRRRSIDNESCASSSDTMANEPGSLCTNPLWNPGPLLSGLLEESSNLPNLEVHMSGG
PFWEEVYGDSILGPPSGSGEHSVL"
gene 10114..11184
/gene="bel2"
CDS 10114..11184
/gene="bel2"
/citation=[5]
/codon_start=1
/product="Bel2"
/protein_id="AAB48116.1"
/translation="MSHVLPVVTPWPMSQDHYAPTLFGILDRYYQGYLKSPATYQTWK
FTCQVDPSGKRFMETQFWVPPLGQVNIQFYKNYQILTCCQAVDPFANIFHGTDEEMFD
IDSGPDVWCSPSLCFKVIYEGAMGQKQEQKTWLCRLGHGHRMGACDYRKVDLYAMRQG
KENPYGDRGDAALQYAYQVKRGCKAGCLASPVLNYKALQFHRTIMADFTNPRIGEGHL
AHGYQAAMEAYGPQRGSNEERVWWNVTRNQGKQGGEYYREGGEEPHYPNTPAPHRRTW
DERHKVLKLSSFATPSDIQRWTTKALPYGWKVVTESGNDYTSRRKIRTLTEMTQDEIR
KRWESGYCDPFIDSGSDSDGPF"
gene 10607..11089
/gene="bel3"
CDS 10607..11089
/gene="bel3"
/citation=[7]
/codon_start=1
/product="Bel3"
/protein_id="AAB48117.1"
/translation="MEIGVMQLCNMLIRLKGAVKQGAWHHLYLTTKLCSFIEPLWQTS
PILGLEKDILLMVTKQLWKLMDLREEVTRRGCGGMSLETRENKEESITGKEVKNLITQ
ILLLLIDVPGMRDTRFLNCPHSLLPLTSNAGLLKHCLMAGKWSPKAEMIILAAERSEH
"
repeat_region 11111..>11954
/citation=[2]
/rpt_type=long_terminal_repeat
regulatory 11175..11181
/regulatory_class="polyA_signal_sequence"
/gene="bel2"
polyA_site 11180
/gene="bel2"
BASE COUNT 4027 a 2169 c 2375 g 3383 t
ORIGIN
1 tgtggtggaa tgccactaga aactagggaa aactaggagg agagtattac agggaaggaa
61 gtgaagaacc tcgtgaccca aatactcctg ctcctcatag acgtacctgg gatgagagac
121 acaaggttct taaattgtcc tcattcgcta ctccctctga catccaacgc tgggctacta
181 aagcattgcc ttatggctgg aaagtggtca ccgaaagcgg aaatgattat actagccgca
241 gaaagatcag aacattgaca gagatgactc aggatgaaat tagaaaaagg tgggaaagtg
301 gatattgtga ccccttcatt gactcaggaa gtgactcaga tggacccttc taaaagccac
361 agacagtaaa aatgtgttag cactttatac aatattatat ctgcttaagc tatagaagct
421 ttcacatact cagtagctgt ttcacaatca acaaaacaat gatgatgtaa tcataaggaa
481 gtagtttaaa ataggttaat aagtttatta gttatataga aaataatata ggataaagta
541 taaggattaa ggtatgaggt gtgtggctca acacgtaggg tgacaagaaa atctactgta
601 ataggacaca acacctctaa agttgcccgt gggaaggtga agtgagatcg aatctttcct
661 taacgcagac agctttttat ccactaggga taatgtttta aggaatacta tagtaataga
721 ttgatagttt taacaatgat ggaaatagta tataaggata gtttctagat tgtacgggag
781 ctcttcacta ctcgctgcgt cgagagtgta cgagactctc caggtttggt aagaaatatt
841 ttatattgtt ataatgttac tatgatccat taacactctg cttatagatt gtaagggtga
901 ttgcaatgct ttctgcataa aactttggtt ttcttgttaa tcaataaacc gacttgattc
961 gagaacctac tcatatatta ttgtctcttt tatactttat taagtaaaag gatttgtata
1021 ttagccttgc taagggagac atctagtgat ataagtgtga actacactta tcttaaatga
1081 tgtaactcct taggataatc aatatacaaa attccatgac aattggcgcc caacgtgggg
1141 ctcgaatata agtcgggttt atttgtaaat tatccctagg gacctccgag catagcggga
1201 ggcatataaa agccaataga caatggcttc aggaagtaat gttgaagaat atgaacttga
1261 tgttgaagct ctggttgtaa ttttaagaga tagaaatata ccaagaaatc ctttacatgg
1321 agaagttata ggtcttcgcc ttactgaagg atggtgggga caaattgaga gatttcagat
1381 ggtacgtcta atattacaag atgatgataa tgaaccttta cagagaccta gatatgaggt
1441 aatacaacga gctgtaaacc ctcatacaat gtttatgata tcaggaccat tagctgaact
1501 tcaattagcc tttcaggatt tagatttacc tgaaggtcca ttgaggtttg gtccattggc
1561 aaatggacat tatgttcaag gagatcctta tagtagttct tacagaccag taacaatggc
1621 cgaaacagcc caaatgacta gagatgaact ggaagatgtt cttaatactc aaagtgaaat
1681 agaaattcaa atgataaatt tattggagtt gtatgaagtt gaaactagag ctcttagaag
1741 acaattagct gagagatcta gtacagggca aggaggaata tccccaggag ctcctcgttc
1801 tcgaccacca gtaagcagct tctcagggtt accaagtttg ccctctatac ctgggattca
1861 tcccagggca ccttcacctc caagggcaac ttctactccc ggaaatattc cttggagttt
1921 aggagatgat agcccacctt catctagttt tcctggaccc tctcaacctc gtgtttcttt
1981 ccatccggga aatccttttg ttgaagaaga aggtcataga cctagatccc agtctagaga
2041 aaggagaaga gaaattcttc ctgctcctgt accgtcagca cctcctatga ttcagtatat
2101 accagtacca cctccaccac cgattggcac ggttatacct attcagcata tcagatctgt
2161 aactggagag cctcctagaa acccaagaga aataccaatt tggctaggac gaaatgctcc
2221 tgctatagat ggagtgttcc ctgttacaac accggatcta agatgcagaa taattaatgc
2281 tatactagga ggaaatattg ggctatcatt aacccctgga gactgtttaa catgggactc
2341 agcagtagcc accttattta ttagaaccca tggaactttt ccaatgcatc agcttggaaa
2401 tgtaataaaa ggcatagttg atcaagaagg agtggcaaca gcatatactt tgggaatgat
2461 gctttctgga caaaattatc aattagtttc tggaataatt agaggatatt tgcctggaca
2521 agctgtagta actgcattac aacagcgttt agaccaagaa atagataatc aaacaagagc
2581 agagactttt attcaacatc taaatgctgt atatgaaatt ttaggcctta atgccagagg
2641 acaaagtata cgtgcttcag tgactcctca accccgacca tccagaggta gaggtcgagg
2701 tcaaaatact tctagaccct ctcaaggacc agctaatagc gggcggggac gacagcgccc
2761 tgcttctggt caaagcaaca gaggatctag tactcagaat caaaatcaag ataatttaaa
2821 tcaaggagga tataatcttc gaccccgtac ttaccaacct caaaggtacg gaggaggacg
2881 tggacgaaga tggaacgata atactaacaa tcaagagtcc agaccatcag atcaaggttc
2941 tcaaactcct aggccaaatc aagcaggctc tggggtgcgt ggcaatcagt cacaaactcc
3001 cagaccagct gctggtcgcg gaggaagagg taaccacaac cgaaaccaac gatcatccgg
3061 tgctggtgac tcacgcgctg tcaataccgt gacacagagt gccacgtcct ccacagatga
3121 atcctcttca gctgttacag ccgcttccgg cggagatcaa agggactaaa ttgttagccc
3181 actgggattc aggggcaaca ataacttgta ttcctgaaag ttttttagaa gatgaacaac
3241 ctattaaaaa gactttaata aaaacaattc atggagaaaa acaacaaaat gtttattatg
3301 taacctttaa agttaaagga agaaaagtgg aagcagaagt gatagcttct ccttatgagt
3361 atattttgct gtcgccaaca gatgttcctt ggttaacaca gcaaccactt cagttaacaa
3421 ttttagttcc tcttcaagaa tatcaagaga aaatcttaag taagactgct cttccagaag
3481 atcaaaaaca acaattaaaa accttgtttg tcaagtatga caatctatgg caacattggg
3541 aaaatcaagt cgggcataga aaaattaggc cacataatat agcaactggt gattatcctc
3601 ctcgccctca aaaacaatat cctattaatc ctaaggcaaa gcctagtata caaattgtaa
3661 tagatgactt attgaaacaa ggggtgttaa cgcctcaaaa tagtacaatg aatacaccag
3721 tgtatcctgt tcctaaacca gatggaaggt ggagaatggt attagattat agagaagtaa
3781 ataaaactat tccattaaca gctgcccaaa accaacactc tgctggtatt ttagctacta
3841 ttgttagaca aaaatataaa actaccttag atttagctaa tggattttgg gctcatccta
3901 ttacaccaga atcttattgg ttaacagcat ttacctggca aggtaaacag tattgttgga
3961 cacgtcttcc tcaaggattt ttaaatagtc cagcattgtt tacagctgat gtagtagatt
4021 tactaaaaga aatccctaat gtacaagtgt atgttgatga tatatattta agccatgatg
4081 atcctaaaga gcatgttcaa caattagaaa aagtgtttca aattttacta caggcaggat
4141 atgtagtatc tttgaaaaaa tcagaaattg gtcaaaaaac tgtagaattt ttaggattta
4201 atattactaa agaaggtcgt ggcctaacag acacttttaa aacaaaactg ttaaatatta
4261 ctcctccaaa agacttaaag caattacaaa gcatattagg attgttaaat tttgctagaa
4321 attttatacc taattttgct gaactggtac aaccattata caatttaata gcctcagcaa
4381 aaggcaaata tattgagtgg tctgaagaaa atactaaaca attaaatatg gtaatagaag
4441 cattaaacac tgcctctaat ttagaagaaa ggttaccaga acagagactg gtaattaaag
4501 tcaatacttc tccatcagca ggatatgtaa gatattataa tgagactggt aaaaagccta
4561 ttatgtacct aaattatgtg ttttccaaag cagaattaaa attttctatg ttagaaaaac
4621 tattaactac aatgcacaaa gccttaatta aggctatgga tttggccatg ggacaagaaa
4681 tattagttta tagtcccatt gtatctatga ctaaaataca aaaaactcca ctaccagaaa
4741 gaaaagcttt acccattaga tggataacat ggatgactta tttagaagat ccaagaatcc
4801 aatttcatta tgataaaacc ttaccagaac ttaagcatat tccagatgta tatacatcta
4861 gtcagtctcc tgttaaacat ccttctcaat atgaaggagt gttttatact gatggctcgg
4921 ccatcaaaag tcctgatcct acaaaaagca ataatgctgg catgggaata gtacatgcca
4981 catacaaacc tgaatatcaa gttttgaatc aatggtcaat accactaggt aatcatactg
5041 ctcagatggc tgaaatagct gcagttgaat ttgcctgtaa aaaagcttta aaaatacctg
5101 gtcctgtatt agttataact gatagtttct atgtagcaga aagtgctaat aaagaattac
5161 catactggaa atctaatggg tttgttaata ataagaaaaa gcctcttaaa catatctcca
5221 aatggaaatc tattgctgag tgtttatcta tgaaaccaga cattactatt caacatgaaa
5281 aaggcatcag cctacaaata ccagtattca tactgaaagg caatgcccta gcagataagc
5341 ttgccaccca aggaagttat gtggttaatt gtaataccaa aaaaccaaac ctggatgcag
5401 agttggatca attattacag ggtcattata taaaaggata tcccaaacaa tatacatatt
5461 ttttagaaga tggcaaagta aaagtttcca gacctgaagg ggttaaaatt attccccctc
5521 agtcagacag acaaaaaatt gtgcttcaag cccacaattt ggctcacacc ggacgtgaag
5581 ccactctttt aaaaattgcc aacctttatt ggtggccaaa tatgagaaag gatgtggtta
5641 aacaactagg acgctgtcaa cagtgtttaa tcacaaatgc ttccaacaaa gcctctggtc
5701 ctattctaag accagatagg cctcaaaaac cttttgataa attctttatt gactatattg
5761 gacctttgcc accttcacag ggatacctat atgtattagt agttgttgat ggaatgacag
5821 gattcacttg gttatacccc actaaggctc cttctactag cgcaactgtt aaatctctca
5881 atgtactcac tagtattgca attccaaagg tgattcactc tgatcaaggt gcagcattca
5941 cttcttcaac ctttgctgaa tgggcaaagg aaagaggtat acatttggaa ttcagtactc
6001 cttatcaccc ccaaagtggt agtaaggtgg aaaggaaaaa tagtgatata aaacgacttt
6061 taactaaact gctagtagga agacccacaa agtggtatga cctattgcct gttgtacaac
6121 ttgctttaaa caacacctat agccctgtat taaaatatac tccacatcaa ctcttatttg
6181 gtatagattc aaatactcca tttgcaaatc aagatacact tgacttgacc agagaagaag
6241 aactttctct tttacaggaa attcgtactt ctttatacca tccatccacc cctccagcct
6301 cctctcgttc ctggtctcct gttgttggcc aattggtcca ggagagggtg gctaggcctg
6361 cttctttgag acctcgttgg cataaaccgt ctactgtact taaggtgttg aatccaagga
6421 ctgttgttat tttggaccat cttggcaaca acagaactgt aagtatagat aatttaaaac
6481 ctacttctca tcagaatggc accaccaatg acactgcaac aatggatcat ttggaaaaaa
6541 atgaataaag cgcatgaggc acttcaaaat acaacaactg tgactgaaca gcagaaggaa
6601 caaattatac tggacattca aaatgaagaa gtacaaccaa ctaggagaga taaatttaga
6661 tatctgcttt atacttgttg tgctactagc tcaagagtat tggcctggat gtttttagtt
6721 tgtatattgt taatcattgt tttggtttca tgctttgtga ctatatccag aatacaatgg
6781 aataaggata ttcaggtatt aggacctgta atagactgga atgttactca aagagctgtt
6841 tatcaaccct tacagactag aaggattgca cgttccctta gaatgcagca tcctgttcca
6901 aaatatgtgg aggtaaatat gactagtatt ccacaaggtg tatactatga accccatccg
6961 gaacccatag tggtgaagga gagggtccta ggtctttctc aaattctgat gattaattca
7021 gaaaacattg ctaataatgc taatttgaca caagaagtaa agaagttgtt aactgaaatg
7081 gttaatgaag aaatgcaaag tttgtcagat gtaatgattg actttgaaat tcctttagga
7141 gaccctcgtg atcaagaaca atatatacat agaaaatgct atcaagaatt tgcaaattgt
7201 tatttagtaa aatataaaga acccaaaccg tggcctaagg agggccttat agctgatcaa
7261 tgcccattac caggttacca tgctggatta acctataata gacagtctat ttgggattac
7321 tatattaaag tggagagtat tagacctgca aattggacaa caaagagtaa atatggacaa
7381 gctagactag gaagttttta tattcctagc agcctgagac aaatcaatgt tagtcatgta
7441 ctattctgta gtgatcaatt atattctaaa tggtataata tagaaaatac catagaacaa
7501 aacgagcggt ttctgcttaa taaactaaat aaccttacat ctggaacctc agtattgaag
7561 aaaagagctc ttccgaagga ttggagttct caaggtaaaa atgctctgtt tagagaaatc
7621 aatgtgttag atatctgcag taaacctgaa tctgtaatac tattgaatac ttcatactat
7681 tccttctctt tatgggaagg agattgtaat tttactaaag atatgatttc tcagttggtt
7741 ccagaatgtg atggatttta taacaattct aagtggatgc atatgcatcc atatgcttgt
7801 agattctgga gaagtaagaa gaatgaaaaa gaagaaacta aatgtagaga tggggaaact
7861 aagagatgtc tgtattatcc tttatgggac agtcccgaat ctacatatga ttttggttat
7921 ttagcatacc aaaagaattt tccttcccct atctgtatag aacaacagaa aattagagat
7981 caagattatg aagtctattc tttgtatcaa gaacgcaaaa tagcttctaa agcatatgga
8041 attgatacag ttttattctc tctaaagaat tttcttaatt atacaggaac tcctgtaaat
8101 gaaatgccta atgcaagagc ttttgtaggc ctaatagatc ccaagtttcc tccttcctat
8161 cccaatgtta ctagggaaca ttatacttcc tgtaataata ggaaaagaag aagtgttgat
8221 aataactatg ctaagttaag gtctatgggg tatgcactta caggagcagt gcaaacctta
8281 tctcaaatat cagatattaa tgatgaaaac ttacagcaag gaatatattt attaagggat
8341 catgtaataa ccttaatgga agctacattg catgatatat ctgttatgga aggaatgttt
8401 gctgtacaac atttgcatac acatttgaat catttgaaga caatgcttct agaaagaaga
8461 atagactgga cctatatgtc tagtacttgg ctacaacaac aattacagaa atctgatgat
8521 gagatgaaag taataaagag aattgctaga agtttggtat attatgttaa acaaacccat
8581 agttctccca cagctacagc ctgggagatt ggattatatt atgaattggt tatacctaaa
8641 catatttact tgaataattg gaatgttgtc aatataggtc acttagttaa atcagctgga
8701 caattgactc atgtaactat agctcatcct tatgaaataa tcaataagga atgtgtagag
8761 actatatatc tgcatcttga ggactgcaca agacaagatt atgtcatatg tgatgtggta
8821 aagatagtgc agccttgtgg caatagctca gacacgagtg attgtcctgt ctgggctgaa
8881 gctgtaaaag aaccatttgt gcaagtcaat cctctgaaaa acggaagtta tctggttttg
8941 gcaagttcca cagactgtca gatcccacca tatgttccta gcatcgtgac tgttaatgaa
9001 acaacgtcat gctttggact ggactttaaa aggccactgg ttgcggaaga aagattgagc
9061 tttgagccac gactgccaaa tctacaacta agattaccac atttggttgg aattattgca
9121 aaaatcaaag ggataaaaat agaagtcaca tcctctggag aaagtataaa agagcagatt
9181 gaaagagcaa aagctgagct ccttcgactg gacattcacg agggagatac tcctgcctgg
9241 atacaacagc tagctgcagc aacaaaggac gtctggccag cagcagcttc tgctctacaa
9301 ggaattggta actttttatc tgggactgcc caaggaatat ttggaactgc ctttagtctc
9361 ttgggatact taaagcctat cctaatagga gtaggggtca ttctcttggt tattcttata
9421 tttaagattg tatcatggat tcctacgaaa aagaagaatc agtagcctcc acctctggaa
9481 ttcaggacct gcagactctg agtgagcttg ttggccctga aaatgccgga gagggagagc
9541 tgactattgc tgaggaacct gaagaaaatc ctcgacgccc cagacgatat accaaaagag
9601 aagtcaaatg tgtgtcttat catgcatata aagaaattga ggacaaacat cctcaacata
9661 ttaaactgca ggattggatc cccacaccag aggaaatgag taagtcactc tgtaaaagac
9721 ttattttatg tggattgtat agtgcagaaa aggcctcaga gattttaagg atgcctttta
9781 cagtttcttg ggaacaatca gatactgacc ctgactgttt tattgtaagc tatacatgta
9841 tattttgtga tgctgtaata catgatccca tgcccataag atgggatcct gaagttggaa
9901 tttgggtaaa atataaaccc ctcagaggaa ttgttggatc tgctgtgttt attatgcata
9961 aacatcaaag aaactgttct cttgttaaac cttctaccag ttgctcagaa ggtccaaaac
10021 caagacctag gcacgatcct gtccttcgat gtgacatgtt tgaaaagcat cacaagcctc
10081 ggcagaaacg acccaggaga cgatccatcg ataatgagtc atgtgcttcc agtagtgaca
10141 ccatggccaa tgagccagga tcactatgca ccaaccctct ttggaatcct ggaccgctac
10201 tatcagggct acttgaagag tccagcaacc taccaaactt ggaagttcac atgtcaggtg
10261 gacccttctg ggaagaggtt tatggagact caattttggg tcccccctct gggtcaggtg
10321 aacattcagt tttataagaa ttatcagatt ctaacttgct gtcaggctgt agacccattt
10381 gctaatattt ttcatggtac tgatgaagaa atgtttgaca ttgattcagg tcctgatgtt
10441 tggtgttctc cctctttgtg tttcaaggta atttatgaag gggcaatggg ccaaaagcaa
10501 gaacaaaaaa cctggctgtg cagactagga catggtcatc gtatgggagc atgcgattac
10561 cgtaaggtag atctgtatgc aatgagacaa ggaaaagaaa acccttatgg agataggggt
10621 gatgcagctt tgcaatatgc ttatcaggtt aaaaggggct gtaaagcagg gtgcttggca
10681 tcacctgtac ttaactacaa agctttgcag tttcatagaa ccattatggc agacttcacc
10741 aatcctagga ttggagaagg acatcttgct catggttacc aagcagctat ggaagcttat
10801 ggacctcaga gaggaagtaa cgaggagagg gtgtggtgga atgtcactag aaaccaggga
10861 aaacaaggag gagagtatta cagggaagga ggtgaagaac ctcattaccc aaatactcct
10921 gctcctcata gacgtacctg ggatgagaga cacaaggttc ttaaattgtc ctcattcgct
10981 actccctctg acatccaacg ctggactact aaagcattgc cttatggctg gaaagtggtc
11041 accgaaagcg gaaatgatta tactagccgc agaaagatca gaacattgac agagatgact
11101 caggatgaaa ttagaaaaag gtgggaaagt ggatattgtg accccttcat tgactcagga
11161 agtgactcag atggaccctt ctaaaagcca cagacagtaa aaatgtgtta gcactttata
11221 caatattata tctgcttaag ctatagaagc tttcacatac tcagtagctg tttcacaatc
11281 aacaaaacaa tgatgatgta atcataagga agtagtttaa aataggttaa gtaagtttat
11341 tagttatata gaaaataata taggataagg tataaggatt aaggtatgag gtgtgtggct
11401 caacacgtag ggtgacaaga aaatctactg taataggaca caacacctct aaagttgccc
11461 gtgggaaggt gaagtgagat cgaatctttc cttaacgcag acagcttttt atccactagg
11521 gataatgttt taaggaatac tatagtaata gattgatagt tttaacaatg atagaaatag
11581 tatataagga tagtttctag attgtacggg aggctcttca ctactcgctg cgtcgagagt
11641 gtacgagact ctccaggttt ggtaagaaat attttatatt gttataatgt tactatgatc
11701 cattaacact ctgcttatag attgtaaggg tgattgcaat gctttctgca taaaactttg
11761 gttttcttgt taatcaataa accgacttga ttcgagaacc aactcctata ttattgtctc
11821 ttttatactt tattaagtaa aaggatttgt atattagcct tgctaaggga gacatctagt
11881 gatataagtg tgaactacac ttatcttaaa tgatgtaact ccttaggata atcaatatac
11941 aaaattccat gaca
//