LOCUS       HSU20760                5009 bp    mRNA    linear   HUM 02-FEB-1996
DEFINITION  Human extracellular calcium-sensing receptor mRNA, complete cds.
ACCESSION   U20760
VERSION     U20760.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Garrett,J.E., Capuano,I.V., Hammerland,L.G., Hung,B.C., Brown,E.M.,
            Hebert,S.C., Nemeth,E.F. and Fuller,F.
  TITLE     Molecular cloning and functional expression of human parathyroid
            calcium receptor cDNAs
  JOURNAL   J. Biol. Chem. 270 (21), 12919-12925 (1995)
   PUBMED   7759551
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Garrett,J.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-1995) James E. Garrett, Molecular Biology, NPS
            Pharmaceuticals, Inc., 420 Chipeta Way, Salt Lake City, UT 84108,
            USA
FEATURES             Location/Qualifiers
     source          1..5009
                     /db_xref="H-InvDB:HIT000218571"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="phPCaR-5.2"
                     /sex="male"
                     /cell_type="chief cells"
                     /tissue_type="parathyroid"
                     /clone_lib="lambdaZ-hPG2ss"
     5'UTR           1..438
     CDS             439..3705
                     /function="seven transmembrane domain G protein-coupled
                     receptor that senses changes in extracellular calcium
                     concentration"
                     /codon_start=1
                     /product="extracellular calcium-sensing receptor"
                     /protein_id="AAA86504.1"
                     /db_xref="GI:683747"
                     /translation="MAFYSCCWVLLALTWHTSAYGPDQRAQKKGDIILGGLFPIHFGV
                     AAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCN
                     TVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLF
                     YIPQVSYASSSRLLSNKNQFKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDY
                     GRPGIEKFREEAEERDICIDFSELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDL
                     EPLIKEIVRRNITGKIWLASEAWASSSLIAMPQYFHVVGGTIGFALKAGQIPGFREFL
                     KKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFLRGHEESGDRFSNSSTAFR
                     PLCTGDENISSVETPYIDYTHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCA
                     DIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEV
                     GYYNVYAKKGERLFINEEKILWSGFSREPLTFVLSVLQVPFSNCSRDCLAGTRKGIIE
                     GEPTCCFECVECPDGEYSDETDASACNKCPDDFWSNENHTSCIAKEIEFLSWTEPFGI
                     ALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEP
                     QDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFL
                     CTFMQIVICVIWLYTAPPSSYRNQELEDEIIFITCHEGSLMALGFLIGYTCLLAAICF
                     FFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASF
                     GLLACIFFNKIYIILFKPSRNTIEEVRCSTAAHAFKVAARATLRRSNVSRKRSSSLGG
                     STGSTPSSSISSKSNSEDPFPRPERQKQQQPLALTQQEQQQQPLTLPQQQRSQQQPRC
                     KQKVIFGSGTVTFSLSFDEPQKNAMAHRNSTHQNSLEAQKSSDTLTRHQPLLPLQCGE
                     TDLDLTVQETGLQGPVGGDQRPEVEDPEELSPALVVSSSQSFVISGGGSTVTENVVNS
                     "
     misc_feature    2047..2076
                     /note="30 bp insertion due to alternative splice site
                     utilization.  This insertion is unique to the phPCaR-5.2
                     clone, and results in a 10 amino acid in-frame insertion
                     into the CaR protein"
     3'UTR           3706..5009
     polyA_signal    4988..4993
     polyA_site      5009
BASE COUNT         1228 a         1376 c         1223 g         1182 t
ORIGIN      
        1 ggggctgctg tggccggacc cgaaggcggg cgccgggagc gcagcgagcc agacgcgcct
       61 ctccaagacc gtgaccttgg catagggagc ggggctgcgc gcagtcctga gatcagacca
      121 gagctcatcc tcgtggagac ccacggccga ggggccggag ctgcctctgt gcgagggagc
      181 cctggccgcg gcgcagaagg catcacagga ggcctctgca tgatgtggct tccaaagact
      241 caaggaccac ccacattaca agtctggatt gaggaaggca gaaatggaga ttcaaacacc
      301 acgtcttcta ttattttatt aatcaatctg tagacatgtg tccccactgc agggagtgaa
      361 ctgctccaag ggagaaactt ctgggagcct ccaaactcct agctgtctca tcccttgccc
      421 tggagagacg gcagaaccat ggcattttat agctgctgct gggtcctctt ggcactcacc
      481 tggcacacct ctgcctacgg gccagaccag cgagcccaaa agaaggggga cattatcctt
      541 ggggggctct ttcctattca ttttggagta gcagctaaag atcaagatct caaatcaagg
      601 ccggagtctg tggaatgtat caggtataat ttccgtgggt ttcgctggtt acaggctatg
      661 atatttgcca tagaggagat aaacagcagc ccagcccttc ttcccaactt gacgctggga
      721 tacaggatat ttgacacttg caacaccgtt tctaaggcct tggaagccac cctgagtttt
      781 gttgctcaaa acaaaattga ttctttgaac cttgatgagt tctgcaactg ctcagagcac
      841 attccctcta cgattgctgt ggtgggagca actggctcag gcgtctccac ggcagtggca
      901 aatctgctgg ggctcttcta cattccccag gtcagttatg cctcctccag cagactcctc
      961 agcaacaaga atcaattcaa gtctttcctc cgaaccatcc ccaatgatga gcaccaggcc
     1021 actgccatgg cagacatcat cgagtatttc cgctggaact gggtgggcac aattgcagct
     1081 gatgacgact atgggcggcc ggggattgag aaattccgag aggaagctga ggaaagggat
     1141 atctgcatcg acttcagtga actcatctcc cagtactctg atgaggaaga gatccagcat
     1201 gtggtagagg tgattcaaaa ttccacggcc aaagtcatcg tggttttctc cagtggccca
     1261 gatcttgagc ccctcatcaa ggagattgtc cggcgcaata tcacgggcaa gatctggctg
     1321 gccagcgagg cctgggccag ctcctccctg atcgccatgc ctcagtactt ccacgtggtt
     1381 ggcggcacca ttggattcgc tctgaaggct gggcagatcc caggcttccg ggaattcctg
     1441 aagaaggtcc atcccaggaa gtctgtccac aatggttttg ccaaggagtt ttgggaagaa
     1501 acatttaact gccacctcca agaaggtgca aaaggacctt tacctgtgga cacctttctg
     1561 agaggtcacg aagaaagtgg cgacaggttt agcaacagct cgacagcctt ccgacccctc
     1621 tgtacagggg atgagaacat cagcagtgtc gagacccctt acatagatta cacgcattta
     1681 cggatatcct acaatgtgta cttagcagtc tactccattg cccacgcctt gcaagatata
     1741 tatacctgct tacctgggag agggctcttc accaatggct cctgtgcaga catcaagaaa
     1801 gttgaggcgt ggcaggtcct gaagcaccta cggcatctaa actttacaaa caatatgggg
     1861 gagcaggtga cctttgatga gtgtggtgac ctggtgggga actattccat catcaactgg
     1921 cacctctccc cagaggatgg ctccatcgtg tttaaggaag tcgggtatta caacgtctat
     1981 gccaagaagg gagaaagact cttcatcaac gaggagaaaa tcctgtggag tgggttctcc
     2041 agggagccac tcacctttgt gctgtctgtc ctccaggtgc ccttctccaa ctgcagccga
     2101 gactgcctgg cagggaccag gaaagggatc attgaggggg agcccacctg ctgctttgag
     2161 tgtgtggagt gtcctgatgg ggagtatagt gatgagacag atgccagtgc ctgtaacaag
     2221 tgcccagatg acttctggtc caatgagaac cacacctcct gcattgccaa ggagatcgag
     2281 tttctgtcgt ggacggagcc ctttgggatc gcactcaccc tctttgccgt gctgggcatt
     2341 ttcctgacag cctttgtgct gggtgtgttt atcaagttcc gcaacacacc cattgtcaag
     2401 gccaccaacc gagagctctc ctacctcctc ctcttctccc tgctctgctg cttctccagc
     2461 tccctgttct tcatcgggga gccccaggac tggacgtgcc gcctgcgcca gccggccttt
     2521 ggcatcagct tcgtgctctg catctcatgc atcctggtga aaaccaaccg tgtcctcctg
     2581 gtgtttgagg ccaagatccc caccagcttc caccgcaagt ggtgggggct caacctgcag
     2641 ttcctgctgg ttttcctctg caccttcatg cagattgtca tctgtgtgat ctggctctac
     2701 accgcgcccc cctcaagcta ccgcaaccag gagctggagg atgagatcat cttcatcacg
     2761 tgccacgagg gctccctcat ggccctgggc ttcctgatcg gctacacctg cctgctggct
     2821 gccatctgct tcttctttgc cttcaagtcc cggaagctgc cggagaactt caatgaagcc
     2881 aagttcatca ccttcagcat gctcatcttc ttcatcgtct ggatctcctt cattccagcc
     2941 tatgccagca cctatggcaa gtttgtctct gccgtagagg tgattgccat cctggcagcc
     3001 agctttggct tgctggcgtg catcttcttc aacaagatct acatcattct cttcaagcca
     3061 tcccgcaaca ccatcgagga ggtgcgttgc agcaccgcag ctcacgcttt caaggtggct
     3121 gcccgggcca cgctgcgccg cagcaacgtc tcccgcaagc ggtccagcag ccttggaggc
     3181 tccacgggat ccaccccctc ctcctccatc agcagcaaga gcaacagcga agacccattc
     3241 ccacggcccg agaggcagaa gcagcagcag ccgctggccc taacccagca agagcagcag
     3301 cagcagcccc tgaccctccc acagcagcaa cgatctcagc agcagcccag atgcaagcag
     3361 aaggtcatct ttggcagcgg cacggtcacc ttctcactga gctttgatga gcctcagaag
     3421 aacgccatgg cccacaggaa ttctacgcac cagaactccc tggaggccca gaaaagcagc
     3481 gatacgctga cccgacacca gccattactc ccgctgcagt gcggggaaac ggacttagat
     3541 ctgaccgtcc aggaaacagg tctgcaagga cctgtgggtg gagaccagcg gccagaggtg
     3601 gaggaccctg aagagttgtc cccagcactt gtagtgtcca gttcacagag ctttgtcatc
     3661 agtggtggag gcagcactgt tacagaaaac gtagtgaatt cataaaatgg aaggagaaga
     3721 ctgggctagg gagaatgcag agaggtttct tggggtccca gggatgagga atcgccccag
     3781 actcctttcc tctgaggaag aagggataat agacacatca aatgccccga atttagtcac
     3841 accatcttaa atgacagtga attgacccat gttcccttta aaattaaaaa aaagaagagc
     3901 cttgtgtttc tgtggttgca tttgtcaaag cattgagatc tccacggtca gatttgctgt
     3961 tcacccacat ctaatgtctc ttcctctgtt ctatcccacc caacagctca gagatgaaac
     4021 tatggcttta aactaccctc cagagtgtgc agactgatgg gacatcaaat ttgccaccac
     4081 tagagctgag agtctgaaag acagaatgtc accagtcctg cccaatgcct tgacaacaga
     4141 ctgaatttta aatgttcaca acataaggag aatgtatctc ctcctattta tgaaaaccat
     4201 atgatatttt gtctcctacc tgctgctgct attatgtaac atccagaagg tttgcacccc
     4261 tcctatacca tatgtctggt tctgtccagg acatgatact gatgccatgt ttagattcca
     4321 ggatcacaag aatcacctca aattgttagg aagggactgc ataaaccaat gagctgtatc
     4381 tgtaattaat attcctatat gtagctttat ccttaggaaa atgcttctgt tgtaatagtc
     4441 catggacaat ataaactgaa aaatgtcagt ctggtttata taaggcagta ttattgagct
     4501 ctatttcccc accccactat cctcactccc ataagctaag ccttatgtga gccccttcag
     4561 ggactcaagg gtccagaagt ccctcccatc tctaccccaa agaattcctg aagccagatc
     4621 caccctatcc ctgtacagag taagttctca attattggcc tgctaatagc tgctagggta
     4681 ggaaagcgtg gttccaagaa agatccaccc tcaaatgtcg gagctatgtt ccctccagca
     4741 gtggtattaa tactgccggt cacccaggct ctggagccag agagacagac cggggttcaa
     4801 gccatggctt cgtcatttgc aagctgagtg actgtaggca gggaacctta acctctctaa
     4861 gccacagctt cttcatcttt aaaataagga taataatcat tccttcccct cagagctctt
     4921 atgtggatta aacgagataa tgtatataaa gtactttagc ctggtaccta gcacacaata
     4981 agcattcaat aaatattagt taatattat
//