LOCUS HSU20760 5009 bp mRNA linear HUM 02-FEB-1996
DEFINITION Human extracellular calcium-sensing receptor mRNA, complete cds.
ACCESSION U20760
VERSION U20760.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 5009)
AUTHORS Garrett,J.E., Capuano,I.V., Hammerland,L.G., Hung,B.C., Brown,E.M.,
Hebert,S.C., Nemeth,E.F. and Fuller,F.
TITLE Molecular cloning and functional expression of human parathyroid
calcium receptor cDNAs
JOURNAL J. Biol. Chem. 270 (21), 12919-12925 (1995)
PUBMED 7759551
REFERENCE 2 (bases 1 to 5009)
AUTHORS Garrett,J.E.
TITLE Direct Submission
JOURNAL Submitted (07-FEB-1995) James E. Garrett, Molecular Biology, NPS
Pharmaceuticals, Inc., 420 Chipeta Way, Salt Lake City, UT 84108,
USA
FEATURES Location/Qualifiers
source 1..5009
/db_xref="H-InvDB:HIT000218571"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="phPCaR-5.2"
/sex="male"
/cell_type="chief cells"
/tissue_type="parathyroid"
/clone_lib="lambdaZ-hPG2ss"
5'UTR 1..438
CDS 439..3705
/function="seven transmembrane domain G protein-coupled
receptor that senses changes in extracellular calcium
concentration"
/codon_start=1
/product="extracellular calcium-sensing receptor"
/protein_id="AAA86504.1"
/translation="MAFYSCCWVLLALTWHTSAYGPDQRAQKKGDIILGGLFPIHFGV
AAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCN
TVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLF
YIPQVSYASSSRLLSNKNQFKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDY
GRPGIEKFREEAEERDICIDFSELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDL
EPLIKEIVRRNITGKIWLASEAWASSSLIAMPQYFHVVGGTIGFALKAGQIPGFREFL
KKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPLPVDTFLRGHEESGDRFSNSSTAFR
PLCTGDENISSVETPYIDYTHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCA
DIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEV
GYYNVYAKKGERLFINEEKILWSGFSREPLTFVLSVLQVPFSNCSRDCLAGTRKGIIE
GEPTCCFECVECPDGEYSDETDASACNKCPDDFWSNENHTSCIAKEIEFLSWTEPFGI
ALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEP
QDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFL
CTFMQIVICVIWLYTAPPSSYRNQELEDEIIFITCHEGSLMALGFLIGYTCLLAAICF
FFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASF
GLLACIFFNKIYIILFKPSRNTIEEVRCSTAAHAFKVAARATLRRSNVSRKRSSSLGG
STGSTPSSSISSKSNSEDPFPRPERQKQQQPLALTQQEQQQQPLTLPQQQRSQQQPRC
KQKVIFGSGTVTFSLSFDEPQKNAMAHRNSTHQNSLEAQKSSDTLTRHQPLLPLQCGE
TDLDLTVQETGLQGPVGGDQRPEVEDPEELSPALVVSSSQSFVISGGGSTVTENVVNS
"
misc_feature 2047..2076
/note="30 bp insertion due to alternative splice site
utilization. This insertion is unique to the phPCaR-5.2
clone, and results in a 10 amino acid in-frame insertion
into the CaR protein"
3'UTR 3706..5009
regulatory 4988..4993
/regulatory_class="polyA_signal_sequence"
polyA_site 5009
BASE COUNT 1228 a 1376 c 1223 g 1182 t
ORIGIN
1 ggggctgctg tggccggacc cgaaggcggg cgccgggagc gcagcgagcc agacgcgcct
61 ctccaagacc gtgaccttgg catagggagc ggggctgcgc gcagtcctga gatcagacca
121 gagctcatcc tcgtggagac ccacggccga ggggccggag ctgcctctgt gcgagggagc
181 cctggccgcg gcgcagaagg catcacagga ggcctctgca tgatgtggct tccaaagact
241 caaggaccac ccacattaca agtctggatt gaggaaggca gaaatggaga ttcaaacacc
301 acgtcttcta ttattttatt aatcaatctg tagacatgtg tccccactgc agggagtgaa
361 ctgctccaag ggagaaactt ctgggagcct ccaaactcct agctgtctca tcccttgccc
421 tggagagacg gcagaaccat ggcattttat agctgctgct gggtcctctt ggcactcacc
481 tggcacacct ctgcctacgg gccagaccag cgagcccaaa agaaggggga cattatcctt
541 ggggggctct ttcctattca ttttggagta gcagctaaag atcaagatct caaatcaagg
601 ccggagtctg tggaatgtat caggtataat ttccgtgggt ttcgctggtt acaggctatg
661 atatttgcca tagaggagat aaacagcagc ccagcccttc ttcccaactt gacgctggga
721 tacaggatat ttgacacttg caacaccgtt tctaaggcct tggaagccac cctgagtttt
781 gttgctcaaa acaaaattga ttctttgaac cttgatgagt tctgcaactg ctcagagcac
841 attccctcta cgattgctgt ggtgggagca actggctcag gcgtctccac ggcagtggca
901 aatctgctgg ggctcttcta cattccccag gtcagttatg cctcctccag cagactcctc
961 agcaacaaga atcaattcaa gtctttcctc cgaaccatcc ccaatgatga gcaccaggcc
1021 actgccatgg cagacatcat cgagtatttc cgctggaact gggtgggcac aattgcagct
1081 gatgacgact atgggcggcc ggggattgag aaattccgag aggaagctga ggaaagggat
1141 atctgcatcg acttcagtga actcatctcc cagtactctg atgaggaaga gatccagcat
1201 gtggtagagg tgattcaaaa ttccacggcc aaagtcatcg tggttttctc cagtggccca
1261 gatcttgagc ccctcatcaa ggagattgtc cggcgcaata tcacgggcaa gatctggctg
1321 gccagcgagg cctgggccag ctcctccctg atcgccatgc ctcagtactt ccacgtggtt
1381 ggcggcacca ttggattcgc tctgaaggct gggcagatcc caggcttccg ggaattcctg
1441 aagaaggtcc atcccaggaa gtctgtccac aatggttttg ccaaggagtt ttgggaagaa
1501 acatttaact gccacctcca agaaggtgca aaaggacctt tacctgtgga cacctttctg
1561 agaggtcacg aagaaagtgg cgacaggttt agcaacagct cgacagcctt ccgacccctc
1621 tgtacagggg atgagaacat cagcagtgtc gagacccctt acatagatta cacgcattta
1681 cggatatcct acaatgtgta cttagcagtc tactccattg cccacgcctt gcaagatata
1741 tatacctgct tacctgggag agggctcttc accaatggct cctgtgcaga catcaagaaa
1801 gttgaggcgt ggcaggtcct gaagcaccta cggcatctaa actttacaaa caatatgggg
1861 gagcaggtga cctttgatga gtgtggtgac ctggtgggga actattccat catcaactgg
1921 cacctctccc cagaggatgg ctccatcgtg tttaaggaag tcgggtatta caacgtctat
1981 gccaagaagg gagaaagact cttcatcaac gaggagaaaa tcctgtggag tgggttctcc
2041 agggagccac tcacctttgt gctgtctgtc ctccaggtgc ccttctccaa ctgcagccga
2101 gactgcctgg cagggaccag gaaagggatc attgaggggg agcccacctg ctgctttgag
2161 tgtgtggagt gtcctgatgg ggagtatagt gatgagacag atgccagtgc ctgtaacaag
2221 tgcccagatg acttctggtc caatgagaac cacacctcct gcattgccaa ggagatcgag
2281 tttctgtcgt ggacggagcc ctttgggatc gcactcaccc tctttgccgt gctgggcatt
2341 ttcctgacag cctttgtgct gggtgtgttt atcaagttcc gcaacacacc cattgtcaag
2401 gccaccaacc gagagctctc ctacctcctc ctcttctccc tgctctgctg cttctccagc
2461 tccctgttct tcatcgggga gccccaggac tggacgtgcc gcctgcgcca gccggccttt
2521 ggcatcagct tcgtgctctg catctcatgc atcctggtga aaaccaaccg tgtcctcctg
2581 gtgtttgagg ccaagatccc caccagcttc caccgcaagt ggtgggggct caacctgcag
2641 ttcctgctgg ttttcctctg caccttcatg cagattgtca tctgtgtgat ctggctctac
2701 accgcgcccc cctcaagcta ccgcaaccag gagctggagg atgagatcat cttcatcacg
2761 tgccacgagg gctccctcat ggccctgggc ttcctgatcg gctacacctg cctgctggct
2821 gccatctgct tcttctttgc cttcaagtcc cggaagctgc cggagaactt caatgaagcc
2881 aagttcatca ccttcagcat gctcatcttc ttcatcgtct ggatctcctt cattccagcc
2941 tatgccagca cctatggcaa gtttgtctct gccgtagagg tgattgccat cctggcagcc
3001 agctttggct tgctggcgtg catcttcttc aacaagatct acatcattct cttcaagcca
3061 tcccgcaaca ccatcgagga ggtgcgttgc agcaccgcag ctcacgcttt caaggtggct
3121 gcccgggcca cgctgcgccg cagcaacgtc tcccgcaagc ggtccagcag ccttggaggc
3181 tccacgggat ccaccccctc ctcctccatc agcagcaaga gcaacagcga agacccattc
3241 ccacggcccg agaggcagaa gcagcagcag ccgctggccc taacccagca agagcagcag
3301 cagcagcccc tgaccctccc acagcagcaa cgatctcagc agcagcccag atgcaagcag
3361 aaggtcatct ttggcagcgg cacggtcacc ttctcactga gctttgatga gcctcagaag
3421 aacgccatgg cccacaggaa ttctacgcac cagaactccc tggaggccca gaaaagcagc
3481 gatacgctga cccgacacca gccattactc ccgctgcagt gcggggaaac ggacttagat
3541 ctgaccgtcc aggaaacagg tctgcaagga cctgtgggtg gagaccagcg gccagaggtg
3601 gaggaccctg aagagttgtc cccagcactt gtagtgtcca gttcacagag ctttgtcatc
3661 agtggtggag gcagcactgt tacagaaaac gtagtgaatt cataaaatgg aaggagaaga
3721 ctgggctagg gagaatgcag agaggtttct tggggtccca gggatgagga atcgccccag
3781 actcctttcc tctgaggaag aagggataat agacacatca aatgccccga atttagtcac
3841 accatcttaa atgacagtga attgacccat gttcccttta aaattaaaaa aaagaagagc
3901 cttgtgtttc tgtggttgca tttgtcaaag cattgagatc tccacggtca gatttgctgt
3961 tcacccacat ctaatgtctc ttcctctgtt ctatcccacc caacagctca gagatgaaac
4021 tatggcttta aactaccctc cagagtgtgc agactgatgg gacatcaaat ttgccaccac
4081 tagagctgag agtctgaaag acagaatgtc accagtcctg cccaatgcct tgacaacaga
4141 ctgaatttta aatgttcaca acataaggag aatgtatctc ctcctattta tgaaaaccat
4201 atgatatttt gtctcctacc tgctgctgct attatgtaac atccagaagg tttgcacccc
4261 tcctatacca tatgtctggt tctgtccagg acatgatact gatgccatgt ttagattcca
4321 ggatcacaag aatcacctca aattgttagg aagggactgc ataaaccaat gagctgtatc
4381 tgtaattaat attcctatat gtagctttat ccttaggaaa atgcttctgt tgtaatagtc
4441 catggacaat ataaactgaa aaatgtcagt ctggtttata taaggcagta ttattgagct
4501 ctatttcccc accccactat cctcactccc ataagctaag ccttatgtga gccccttcag
4561 ggactcaagg gtccagaagt ccctcccatc tctaccccaa agaattcctg aagccagatc
4621 caccctatcc ctgtacagag taagttctca attattggcc tgctaatagc tgctagggta
4681 ggaaagcgtg gttccaagaa agatccaccc tcaaatgtcg gagctatgtt ccctccagca
4741 gtggtattaa tactgccggt cacccaggct ctggagccag agagacagac cggggttcaa
4801 gccatggctt cgtcatttgc aagctgagtg actgtaggca gggaacctta acctctctaa
4861 gccacagctt cttcatcttt aaaataagga taataatcat tccttcccct cagagctctt
4921 atgtggatta aacgagataa tgtatataaa gtactttagc ctggtaccta gcacacaata
4981 agcattcaat aaatattagt taatattat
//