LOCUS T19026 369 bp mRNA linear EST 25-SEP-1996 DEFINITION c12023t Testis 1 Homo sapiens cDNA clone c12023 5' end, mRNA sequence. ACCESSION T19026 VERSION T19026.1 DBLINK BioSample: SAMN00154734 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 369) AUTHORS Pawlak,A., Toussaint,C., Levy,I., Bulle,F., Poyard,M., Barouki,R. and Guellaen,G. TITLE Characterization of a large population of mRNAs from human testis JOURNAL Genomics 26, 151-158 (1995) PUBMED 7782076 COMMENT Contact: Guellaen G Unite INSERM 99 INSERM Unite INSERM 99, Hopital Henri Mondor, 94010 Creteil, France Tel: (33)149813530 Fax: (33)148980908 Email: guellaen@infobiogen.fr Seq primer: T7. FEATURES Location/Qualifiers source 1..369 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="c12023" /clone_lib="SAMN00154734 Testis 1" /note="Vector: PGEM 5zf(+); Site_1: EcoRV; Site_2: Not1; mRNA was prepared from human testis of a 27 years old man. cDNA was prepared using a 15mer oligo dT anchored by two degenerated bases at its 3'end and containing a NotI site at its 5'end. The cDNA was cloned between EcoRV and NotI sites of PGEM 5zf(+). The 3' end is at the NotI site. The EcoRV site is lost during the cloning procedure. cDNA corresponding to abundant species were eliminated from this library." BASE COUNT 86 a 104 c 87 g 85 t ORIGIN 1 tggcagtggg cggcacagag cacgcctacc ggcccggccg taggtgtgtg ctgtccgggc 61 tacggggacc ctgtctccga gtcgttcgtg cagcgtgtgt accagccctt cctcaccacc 121 tgcgacgggc accggaaaga gaacttggag tatagtttaa caaagatggt tacaggagct 181 tccctatcca catactccta ccaacagcct gtacggccct ctttataacg tctctntccc 241 aaccccatgg cccactncca cagctgacag gtccaagagt gttnctgcct gagctaatga 301 acatnaaaat nacagactgg atcaatttaa ttttcctgtc taaaagcatt gtggaaatta 361 gcangttnt //