LOCUS R10022 375 bp mRNA linear EST 06-APR-1995 DEFINITION yf34h08.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE:128799 5' similar to gb:X15183_cds1 HEAT SHOCK PROTEIN HSP 90-ALPHA (HUMAN), mRNA sequence. ACCESSION R10022 VERSION R10022.1 DBLINK BioSample: SAMN00154876 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 375) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu Insert Size: 1109 High quality sequence stops: 171 Source: IMAGE Consortium, LLNL This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 1109 Std Error: 0.00 Seq primer: M13RP1 High quality sequence stop: 171. FEATURES Location/Qualifiers source 1..375 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:480960" /db_xref="taxon:9606" /clone="IMAGE:128799" /sex="male" /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS" /dev_stage="20 week-post conception fetus" /lab_host="DH10B (ampicillin resistant)" /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Pac I; Site_2: Eco RI; 1st strand cDNA was primed with a Pac I - oligo(dT) primer [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Pac I and cloned into the Pac I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 95 a 79 c 89 g 106 t ORIGIN 1 ctggaccatt ccattattga gaccttaagg caaaaggcag aggctgataa gaacgacaag 61 tctgtgaagg atctggtcat cttgctttat gaaactgcgc tcctgtcttc tggcttcagt 121 ctggaagatc cccagacaca tgctaacagg atctacagga tgatcaaact tggtctgggt 181 attgatgaag atgaccctac tgctgatgat accagtgctg ctgtaactga aggaaatgcc 241 accccttgaa ggagatgacg acacattcac gcatgggagg nagtaggact tatctcttgg 301 cttaggggnt gactttancc tgtttcagta cttctaccat ttcccnctga taatntattt 361 tncaggggtg ttttt //