LOCUS       R10022                   375 bp    mRNA    linear   EST 06-APR-1995
DEFINITION  yf34h08.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone
            IMAGE:128799 5' similar to gb:X15183_cds1 HEAT SHOCK PROTEIN HSP
            90-ALPHA (HUMAN), mRNA sequence.
ACCESSION   R10022
VERSION     R10022.1
DBLINK      BioSample: SAMN00154876
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Insert Size: 1109
            High quality sequence stops: 171 Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1109   Std Error: 0.00
            Seq primer: M13RP1
            High quality sequence stop: 171.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:480960"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:128799"
                     /sex="male"
                     /clone_lib="SAMN00154876 Soares fetal liver spleen 1NFLS"
                     /dev_stage="20 week-post conception fetus"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: Liver and Spleen; Vector: pT7T3D (Pharmacia)
                     with a modified polylinker; Site_1: Pac I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Pac I - oligo(dT) primer
                     [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Pac I and cloned into the Pac I
                     and Eco RI sites of the modified pT7T3 vector. Library
                     went through one round of normalization. Library
                     constructed by Bento Soares and M.Fatima Bonaldo."
BASE COUNT           95 a           79 c           89 g          106 t
ORIGIN      
        1 ctggaccatt ccattattga gaccttaagg caaaaggcag aggctgataa gaacgacaag
       61 tctgtgaagg atctggtcat cttgctttat gaaactgcgc tcctgtcttc tggcttcagt
      121 ctggaagatc cccagacaca tgctaacagg atctacagga tgatcaaact tggtctgggt
      181 attgatgaag atgaccctac tgctgatgat accagtgctg ctgtaactga aggaaatgcc
      241 accccttgaa ggagatgacg acacattcac gcatgggagg nagtaggact tatctcttgg
      301 cttaggggnt gactttancc tgtttcagta cttctaccat ttcccnctga taatntattt
      361 tncaggggtg ttttt
//