LOCUS R04283 312 bp mRNA linear EST 31-MAR-1995 DEFINITION pk22b02.s1 Kuwabara Mixed stage C. briggsae Caenorhabditis briggsae cDNA similar to SP:EF1A_APIME P19039 ELONGATION FACTOR 1-ALPHA, mRNA sequence. ACCESSION R04283 VERSION R04283.1 DBLINK BioSample: SAMN00154883 KEYWORDS EST. SOURCE Caenorhabditis briggsae ORGANISM Caenorhabditis briggsae Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis. REFERENCE 1 (bases 1 to 312) AUTHORS Hillier,L., Chiapelli,B., Chissoe,S., Clark,N., Couch,J., Dubuque,T., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Kuwabara,P., Le,M., Mardis,E., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Tan,F., Trevaskis,E., Waterston,R., Wohldmann,P. and Wilson,R. TITLE Washington University Caenorhabditis briggsae EST project JOURNAL Unpublished COMMENT Other_ESTs: pk22b02.r1 Contact: Marra MA Washington University Genome Sequencing Center Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1455 Fax: 314 286 1810 Email: mmarra@watson.wustl.edu PCR_F: TGTAAAACGACGGCCAGTGAGCAAGTTCAGCCTGG PCR_B: CAGGAAACAGCTATGACCCTTATGAGTATTTCTTCCAGGGTA Source: Washington University Genome Sequencing Center PCR amplified DNA is available from Washington University Genome Sequencing Center. Aliquots of the library may be requested from P. Kuwabara (pek@mrc-lmb.cam.ac.uk). Seq primer: Commercially available -21M13 dye primer. FEATURES Location/Qualifiers source 1..312 /organism="Caenorhabditis briggsae" /mol_type="mRNA" /strain="G16 Gujarat" /db_xref="taxon:6238" /clone_lib="SAMN00154883 Kuwabara Mixed stage C. briggsae" /note="Vector: Lambda gt10; Site_1: EcoRI; Site_2: EcoRI; Stage:mixed, Sex:hermaphrodite. Library construction: First strand oligo(dT) primed. Second strand was as per Gubler/Hoffman. Ligated to EcoRI adaptors. Library is non-directional. Library is non-normalized. Library constructed by P.E. Kuwabara. Additional details on construction of the library are described in P.E. Kuwabara and S. Shah, NAR 22: 4414 - 4418 (1994). Adaptor sequence: GAATTC CGTTGCTGTCG" BASE COUNT 89 a 80 c 79 g 61 t ORIGIN 1 gctcactccg tagcagccat gggaaaagan aangttcana tcaacatcgt cgtcatcgga 61 cacgtcgact ccggaaagtc caccaccacc ggacacttga tctacaagtg cggaggaatt 121 gacaagagaa ccatcgagaa attcgagaag gaggctcagg agatgggtaa gggatctttc 181 aagtacgctt gggtcctcga caagctgaag gccgagcgtg aacgtggtat caccatcgat 241 atcgctctct ggaagttcga gaccgctaag tactacatca ccatcatcga tgccccagga 301 catcgtgatt tc //