LOCUS R04076 368 bp mRNA linear EST 31-MAR-1995 DEFINITION pk19a12.s1 Kuwabara Mixed stage C. briggsae Caenorhabditis briggsae cDNA similar to SP:F56F3.5 CE00664 RIBOSOMAL PROTEIN S3A, mRNA sequence. ACCESSION R04076 VERSION R04076.1 DBLINK BioSample: SAMN00154883 KEYWORDS EST. SOURCE Caenorhabditis briggsae ORGANISM Caenorhabditis briggsae Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis. REFERENCE 1 (bases 1 to 368) AUTHORS Hillier,L., Chiapelli,B., Chissoe,S., Clark,N., Couch,J., Dubuque,T., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Kuwabara,P., Le,M., Mardis,E., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Tan,F., Trevaskis,E., Waterston,R., Wohldmann,P. and Wilson,R. TITLE Washington University Caenorhabditis briggsae EST project JOURNAL Unpublished COMMENT Contact: Marra MA Washington University Genome Sequencing Center Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1455 Fax: 314 286 1810 Email: mmarra@watson.wustl.edu PCR_F: TGTAAAACGACGGCCAGTGAGCAAGTTCAGCCTGG PCR_B: CAGGAAACAGCTATGACCCTTATGAGTATTTCTTCCAGGGTA Source: Washington University Genome Sequencing Center PCR amplified DNA is available from Washington University Genome Sequencing Center. Aliquots of the library may be requested from P. Kuwabara (pek@mrc-lmb.cam.ac.uk). Seq primer: Commercially available -21M13 dye primer. FEATURES Location/Qualifiers source 1..368 /organism="Caenorhabditis briggsae" /mol_type="mRNA" /strain="G16 Gujarat" /db_xref="taxon:6238" /clone_lib="SAMN00154883 Kuwabara Mixed stage C. briggsae" /note="Vector: Lambda gt10; Site_1: EcoRI; Site_2: EcoRI; Stage:mixed, Sex:hermaphrodite. Library construction: First strand oligo(dT) primed. Second strand was as per Gubler/Hoffman. Ligated to EcoRI adaptors. Library is non-directional. Library is non-normalized. Library constructed by P.E. Kuwabara. Additional details on construction of the library are described in P.E. Kuwabara and S. Shah, NAR 22: 4414 - 4418 (1994). Adaptor sequence: GAATTC CGTTGCTGTCG" BASE COUNT 116 a 93 c 87 g 71 t ORIGIN 1 agacacagct tggtatcaat ccgcttcaaa tatggcggtc ggaaagaaca ataacaagat 61 gggaaagaaa ggaggaaaga agaaggccgt ggaccccttc tctcgcaagg agtggtatga 121 catcaaggct ccaaacatgt tcaacacccg tcagttggaa agaccctcat caacagaacc 181 caaggaacca agatcgcttc tgaaggactt aagggacgtg tcttcgaggt ctccctcgga 241 gactntggaa caactctgaa gctgacttca gaaagttcaa gctcatcgct gaagatgtcc 301 aaggaaagaa cgtcctcacc aacttccacg ctatgtcgat gacccacgac aagttgtgct 361 ccatcgtc //