LOCUS       R03918                   304 bp    mRNA    linear   EST 31-MAR-1995
DEFINITION  pk13a12.s1 Kuwabara Mixed stage C. briggsae Caenorhabditis briggsae
            cDNA similar to SP:A27123 A27123 ELONGATION FACTOR TU - MOUSE, mRNA
            sequence.
ACCESSION   R03918
VERSION     R03918.1
DBLINK      BioSample: SAMN00154883
KEYWORDS    EST.
SOURCE      Caenorhabditis briggsae
  ORGANISM  Caenorhabditis briggsae
            Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
            Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
            Caenorhabditis.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Hillier,L., Chiapelli,B., Chissoe,S., Clark,N., Couch,J.,
            Dubuque,T., Hawkins,M., Holman,M., Hultman,M., Kucaba,T.,
            Kuwabara,P., Le,M., Mardis,E., Marra,M., Parsons,J., Rifkin,L.,
            Rohlfing,T., Tan,F., Trevaskis,E., Waterston,R., Wohldmann,P. and
            Wilson,R.
  TITLE     Washington University Caenorhabditis briggsae EST project
  JOURNAL   Unpublished
COMMENT     Other_ESTs: pk13a12.r1
            Contact: Marra MA
            Washington University Genome Sequencing Center
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1455
            Fax: 314 286 1810
            Email: mmarra@watson.wustl.edu
            PCR_F: TGTAAAACGACGGCCAGTGAGCAAGTTCAGCCTGG
            PCR_B: CAGGAAACAGCTATGACCCTTATGAGTATTTCTTCCAGGGTA
            Source: Washington University Genome Sequencing Center
            PCR amplified DNA is available from Washington University Genome
            Sequencing Center. Aliquots of the library may be requested from P.
            Kuwabara (pek@mrc-lmb.cam.ac.uk).
            Seq primer: Commercially available -21M13 dye primer.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Caenorhabditis briggsae"
                     /mol_type="mRNA"
                     /strain="G16 Gujarat"
                     /db_xref="taxon:6238"
                     /clone_lib="SAMN00154883 Kuwabara Mixed stage C. briggsae"
                     /note="Vector: Lambda gt10; Site_1: EcoRI; Site_2: EcoRI;
                     Stage:mixed, Sex:hermaphrodite. Library construction:
                     First strand oligo(dT) primed. Second strand was as per
                     Gubler/Hoffman. Ligated to EcoRI adaptors. Library is
                     non-directional. Library is non-normalized. Library
                     constructed by P.E. Kuwabara. Additional details on
                     construction of the library are described in P.E. Kuwabara
                     and S. Shah, NAR 22: 4414 - 4418 (1994). Adaptor sequence:
                     GAATTC CGTTGCTGTCG"
BASE COUNT           88 a           75 c           78 g           60 t
ORIGIN      
        1 cgtagcagcc atgggaaaag aaaaggttca tatcaacatc gtcgtcatcg gacacgtcga
       61 ctccnggaaa gtccaccacc accnggacac ttgatctaca agtgcggagg aattgacaag
      121 agaaccatcg agaaattcga gaaggaggct caggagatgg gtaagggatc tttcaagtac
      181 gcttgggtcc tcgacaagct gaaggccgag cgtgaacgtg gtatcaccat cgatatcgct
      241 ctctgggaag ttcgagaccg cttaagtact tacattcacc atcatcngat gccccaggac
      301 attc
//