LOCUS R03224 320 bp mRNA linear EST 31-MAR-1995 DEFINITION pk05a12.s1 Kuwabara Mixed stage C. briggsae Caenorhabditis briggsae cDNA similar to SP:EF1A_APIME P19039 ELONGATION FACTOR 1-ALPHA, mRNA sequence. ACCESSION R03224 VERSION R03224.1 DBLINK BioSample: SAMN00154883 KEYWORDS EST. SOURCE Caenorhabditis briggsae ORGANISM Caenorhabditis briggsae Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis. REFERENCE 1 (bases 1 to 320) AUTHORS Hillier,L., Chiapelli,B., Chissoe,S., Clark,N., Couch,J., Dubuque,T., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Kuwabara,P., Le,M., Mardis,E., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Tan,F., Trevaskis,E., Waterston,R., Wohldmann,P. and Wilson,R. TITLE Washington University Caenorhabditis briggsae EST project JOURNAL Unpublished COMMENT Other_ESTs: pk05a12.r1 Contact: Marra MA Washington University Genome Sequencing Center Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1455 Fax: 314 286 1810 Email: mmarra@watson.wustl.edu PCR_F: TGTAAAACGACGGCCAGTGAGCAAGTTCAGCCTGG PCR_B: CAGGAAACAGCTATGACCCTTATGAGTATTTCTTCCAGGGTA Source: Washington University Genome Sequencing Center Putatively trans-spliced (TSL site SL1). PCR amplified DNA is available from Washington University Genome Sequencing Center. Aliquots of the library may be requested from P. Kuwabara (pek@mrc-lmb.cam.ac.uk). Seq primer: Commercially available -21M13 dye primer. FEATURES Location/Qualifiers source 1..320 /organism="Caenorhabditis briggsae" /mol_type="mRNA" /strain="G16 Gujarat" /db_xref="taxon:6238" /clone_lib="SAMN00154883 Kuwabara Mixed stage C. briggsae" /note="Vector: Lambda gt10; Site_1: EcoRI; Site_2: EcoRI; Stage:mixed, Sex:hermaphrodite. Library construction: First strand oligo(dT) primed. Second strand was as per Gubler/Hoffman. Ligated to EcoRI adaptors. Library is non-directional. Library is non-normalized. Library constructed by P.E. Kuwabara. Additional details on construction of the library are described in P.E. Kuwabara and S. Shah, NAR 22: 4414 - 4418 (1994). Adaptor sequence: GAATTC CGTTGCTGTCG" BASE COUNT 63 a 81 c 82 g 92 t ORIGIN 1 tggggcatcg antgatggtg atgtagtact tagcgngtct cgaacttcca gagagcgata 61 tcgatggtga taccacgttc acgctcggcc ttcagcttgt cgaggaccca agcgtacttg 121 aaagatccct tacccatctc ctgagcctcc ttctcgaatt tctcgatggt tctcttgtca 181 attcctccgc acttgtagat caagtgtccg gtggtggtgg actttccgga gtcgacgtgt 241 ccgatgacga cgatgttgat atgaaccttt tcttttccca tggctgctac ggagtgagcc 301 gacagcaacg ggaattcttt //