LOCUS       R03093                   400 bp    mRNA    linear   EST 31-MAR-1995
DEFINITION  pk03g04.s2 Kuwabara Mixed stage C. briggsae Caenorhabditis briggsae
            cDNA similar to SP:SRCH_RABIT P16230 SARCOPLASMIC RETICULUM
            HISTIDINE-RICH CALCIUM-BINDING PROTEIN PRECURSOR, mRNA sequence.
ACCESSION   R03093
VERSION     R03093.1
DBLINK      BioSample: SAMN00154883
KEYWORDS    EST.
SOURCE      Caenorhabditis briggsae
  ORGANISM  Caenorhabditis briggsae
            Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
            Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
            Caenorhabditis.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Hillier,L., Chiapelli,B., Chissoe,S., Clark,N., Couch,J.,
            Dubuque,T., Hawkins,M., Holman,M., Hultman,M., Kucaba,T.,
            Kuwabara,P., Le,M., Mardis,E., Marra,M., Parsons,J., Rifkin,L.,
            Rohlfing,T., Tan,F., Trevaskis,E., Waterston,R., Wohldmann,P. and
            Wilson,R.
  TITLE     Washington University Caenorhabditis briggsae EST project
  JOURNAL   Unpublished
COMMENT     Other_ESTs: pk03g04.r1
            Contact: Marra MA
            Washington University Genome Sequencing Center
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1455
            Fax: 314 286 1810
            Email: mmarra@watson.wustl.edu
            PCR_F: TGTAAAACGACGGCCAGTGAGCAAGTTCAGCCTGG
            PCR_B: CAGGAAACAGCTATGACCCTTATGAGTATTTCTTCCAGGGTA
            Source: Washington University Genome Sequencing Center
            PCR amplified DNA is available from Washington University Genome
            Sequencing Center. Aliquots of the library may be requested from P.
            Kuwabara (pek@mrc-lmb.cam.ac.uk).
            Seq primer: Commercially available -21M13 dye primer.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Caenorhabditis briggsae"
                     /mol_type="mRNA"
                     /strain="G16 Gujarat"
                     /db_xref="taxon:6238"
                     /clone_lib="SAMN00154883 Kuwabara Mixed stage C. briggsae"
                     /note="Vector: Lambda gt10; Site_1: EcoRI; Site_2: EcoRI;
                     Stage:mixed, Sex:hermaphrodite. Library construction:
                     First strand oligo(dT) primed. Second strand was as per
                     Gubler/Hoffman. Ligated to EcoRI adaptors. Library is
                     non-directional. Library is non-normalized. Library
                     constructed by P.E. Kuwabara. Additional details on
                     construction of the library are described in P.E. Kuwabara
                     and S. Shah, NAR 22: 4414 - 4418 (1994). Adaptor sequence:
                     GAATTC CGTTGCTGTCG"
BASE COUNT           77 a          103 c           76 g          142 t
ORIGIN      
        1 ntacaatcaa agtgcgaata gaaattgggg tgtagggggt aattagtgtc taattaatga
       61 gcaattatcc aaagtgcaat tagagagaag caaaaagttc ccgttgcgcg gtgaaggctg
      121 atggaaccat tgtcctcacc gtcatctcca ttatcgtcgt cgtcttcatc tgaatccttc
      181 tttttccggt tttccttttt catcgtcttc ttttttggct tttcttcttc ctcttcctca
      241 tcgttgtcat cagacttgcc acgtcgagga tgtcgggacg tcttcttttc ggaacgtctc
      301 cttcttccat cggatcaaac tcctcctcct cctcttcttc atccttctca tatctcgacg
      361 gacgacctct tctagatgtt gggcttcttc ttcttacgng
//