LOCUS       R02924                   352 bp    mRNA    linear   EST 31-MAR-1995
DEFINITION  pk02e08.s3 Kuwabara Mixed stage C. briggsae Caenorhabditis briggsae
            cDNA similar to SP:NUCL_MESAU P08199 NUCLEOLIN, mRNA sequence.
ACCESSION   R02924
VERSION     R02924.1
DBLINK      BioSample: SAMN00154883
KEYWORDS    EST.
SOURCE      Caenorhabditis briggsae
  ORGANISM  Caenorhabditis briggsae
            Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
            Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
            Caenorhabditis.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Hillier,L., Chiapelli,B., Chissoe,S., Clark,N., Couch,J.,
            Dubuque,T., Hawkins,M., Holman,M., Hultman,M., Kucaba,T.,
            Kuwabara,P., Le,M., Mardis,E., Marra,M., Parsons,J., Rifkin,L.,
            Rohlfing,T., Tan,F., Trevaskis,E., Waterston,R., Wohldmann,P. and
            Wilson,R.
  TITLE     Washington University Caenorhabditis briggsae EST project
  JOURNAL   Unpublished
COMMENT     Contact: Marra MA
            Washington University Genome Sequencing Center
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1455
            Fax: 314 286 1810
            Email: mmarra@watson.wustl.edu
            PCR_F: TGTAAAACGACGGCCAGTGAGCAAGTTCAGCCTGG
            PCR_B: CAGGAAACAGCTATGACCCTTATGAGTATTTCTTCCAGGGTA
            Source: Washington University Genome Sequencing Center
            Putatively trans-spliced (TSL site SL1).
             PCR amplified DNA is available from Washington University Genome
            Sequencing Center. Aliquots of the library may be requested from P.
            Kuwabara (pek@mrc-lmb.cam.ac.uk).
            Seq primer: Commercially available -21M13 dye primer.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Caenorhabditis briggsae"
                     /mol_type="mRNA"
                     /strain="G16 Gujarat"
                     /db_xref="taxon:6238"
                     /clone_lib="SAMN00154883 Kuwabara Mixed stage C. briggsae"
                     /note="Vector: Lambda gt10; Site_1: EcoRI; Site_2: EcoRI;
                     Stage:mixed, Sex:hermaphrodite. Library construction:
                     First strand oligo(dT) primed. Second strand was as per
                     Gubler/Hoffman. Ligated to EcoRI adaptors. Library is
                     non-directional. Library is non-normalized. Library
                     constructed by P.E. Kuwabara. Additional details on
                     construction of the library are described in P.E. Kuwabara
                     and S. Shah, NAR 22: 4414 - 4418 (1994). Adaptor sequence:
                     GAATTC CGTTGCTGTCG"
BASE COUNT          135 a           57 c          113 g           45 t
ORIGIN      
        1 gaggatgagg aagacgaaga ggaggncgag agtgatgacg aacctcaacc acagaagaag
       61 gtcgtgaaag gagcacttgc cgctgctgct gaagactccg acgacgacga agaggaagat
      121 gacgaggagg angaagaaga gtccgagaat gaaggcccag taacaaagaa agtcatctct
      181 gttaaagaag ttgacgatga agatgacgaa gacgaggagg acgaagaaga agtcgaagtg
      241 gacgacgaag atgaggaaga ggaggaagac gaagaagaag aggagccagt agctaaaact
      301 ccagctttga aaagtgctct tgaaaactct cgattccgct taaaggaaaa ca
//