LOCUS N24005 460 bp mRNA linear EST 28-DEC-1995
DEFINITION yx87e09.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone
IMAGE:268744 3' similar to gb:K00558 TUBULIN ALPHA-1 CHAIN (HUMAN),
mRNA sequence.
ACCESSION N24005
VERSION N24005.1
DBLINK BioSample: SAMN00154979
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 460)
AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
Wilson,R.
TITLE The WashU-Merck EST Project
JOURNAL Unpublished
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
High quality sequence stops: 411
Source: IMAGE Consortium, LLNL
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Seq primer: m13 -40 forward
High quality sequence stop: 411.
FEATURES Location/Qualifiers
source 1..460
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3878386"
/db_xref="taxon:9606"
/clone="IMAGE:268744"
/sex="male"
/tissue_type="melanocyte"
/clone_lib="SAMN00154979 Soares melanocyte 2NbHM"
/lab_host="DH10B (ampicillin resistant)"
/note="Vector: pT7T3D (Pharmacia) with a modified
polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library constructed by Bento Soares and
M.Fatima Bonaldo. RNA from normal foreskin melanocytes
(FS374) was kindly provided by Dr. Anthony P. Albino."
BASE COUNT 128 a 128 c 97 g 107 t
ORIGIN
1 gatgttaatg actttacttt gagatatgat ggaaaaatat tacaggtaca catggaaaag
61 acatgatcac caagtgaaaa caatctaacc agaaagcttt aacgtctgtc agttaagctg
121 aagctgaaat tctgggagca tgacatgctg cagggccaaa aggaatggat aattagtatt
181 cctctccttc ttcctcaccc tctccttcaa cagaatccac accaacctcc tcataatcct
241 tctcaagggc agccatatct tcacgggcct ctgaaaactc gccttcctcc atcccctcac
301 ccacgtacca gtgaacaaag gcacgcttgg catacatcag gtcaaacttg tggtccaggc
361 gagcccaggc ctcagcaatg gctgtggtgt tgctcagcat gcacacagct ctctggtacc
421 ttgggccagg tctccaccag gcaccacagt gggaggctgg
//