LOCUS N22921 478 bp mRNA linear EST 28-DEC-1995 DEFINITION yx66h02.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:266739 3', mRNA sequence. ACCESSION N22921 VERSION N22921.1 DBLINK BioSample: SAMN00154979 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 478) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu High quality sequence stops: 339 Source: IMAGE Consortium, LLNL This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: m13 -40 forward High quality sequence stop: 339. FEATURES Location/Qualifiers source 1..478 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:3876381" /db_xref="taxon:9606" /clone="IMAGE:266739" /sex="male" /tissue_type="melanocyte" /clone_lib="SAMN00154979 Soares melanocyte 2NbHM" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from normal foreskin melanocytes (FS374) was kindly provided by Dr. Anthony P. Albino." BASE COUNT 152 a 83 c 71 g 171 t ORIGIN 1 gctttaagtt ttaatgttta tttccccaag acagcctagc ctgcactcta cttggataaa 61 ttttacaagc tagttttctg ctgcttctag ttttaaactt taaccatgtt tctgatgaca 121 aggaatgctg caaaaatact ctagttcaac aaagagttat gatcacaaaa taatttttat 181 ccattctaca gtgtttcaga attaccagtt gatttttaaa cacaaagtag atatagatgc 241 taatggtggc taatctggta tgtttcttat agcaaactgt tgttcatgca acacttgtgc 301 tcaaagggga aggcacagga tttcctacaa tgagccacct tataaagagt tctttttgta 361 caaattaatt tatgtcacat ttaatttagt gtgcataacc tcaaactgag gtattattcc 421 natttataaa aaccacttgc ataactttta tgccagtttt taaaaataca aagttttc //