LOCUS N21004 411 bp mRNA linear EST 19-DEC-1995 DEFINITION yx57h03.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:265877 3', mRNA sequence. ACCESSION N21004 VERSION N21004.1 DBLINK BioSample: SAMN00154979 KEYWORDS EST. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 411) AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and Wilson,R. TITLE The WashU-Merck EST Project JOURNAL Unpublished COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu High quality sequence stops: 183 Source: IMAGE Consortium, LLNL This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: m13 -40 forward High quality sequence stop: 183. FEATURES Location/Qualifiers source 1..411 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="GDB:3875519" /db_xref="taxon:9606" /clone="IMAGE:265877" /sex="male" /tissue_type="melanocyte" /clone_lib="SAMN00154979 Soares melanocyte 2NbHM" /lab_host="DH10B (ampicillin resistant)" /note="Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from normal foreskin melanocytes (FS374) was kindly provided by Dr. Anthony P. Albino." BASE COUNT 137 a 69 c 71 g 129 t ORIGIN 1 acatcaaaat gtttattttt tggatataga catttaaaac attcatctcc aagaacagct 61 tcaatcaggt atacaataag aaatagactt taaatcctaa tacagaaaag gtaacagaat 121 ttttttaaat gctgctgtag acactagtgt tagaaaaaaa agtttataaa tggttttgca 181 ccaaaaaaat atgcaaagaa acttgaaaat agaaattgtt tgccatttng ttgctctggt 241 tgctggtnta atatagaccc agtgactgat tgtcttcagc acacatctaa ttttggccat 301 ttacctgcaa tttggcttat gtgatccnaa aatggtgatc tagatagtgc ttttcactgg 361 gagggantgg ccaaccacgc tacattgcct ttaggtggat cctccatncc a //