LOCUS       N20159                   457 bp    mRNA    linear   EST 18-DEC-1995
DEFINITION  yx41b05.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone
            IMAGE:264273 3', mRNA sequence.
ACCESSION   N20159
VERSION     N20159.1
DBLINK      BioSample: SAMN00154979
KEYWORDS    EST.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
            Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
            Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
            Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
            Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            High quality sequence stops: 367
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Insert Length: 1437   Std Error: 0.00
            Seq primer: m13 -40 forward
            High quality sequence stop: 367.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="GDB:3873915"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:264273"
                     /sex="male"
                     /tissue_type="melanocyte"
                     /clone_lib="SAMN00154979 Soares melanocyte 2NbHM"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Vector: pT7T3D (Pharmacia) with a modified
                     polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA
                     was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCAGTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library constructed by Bento Soares and
                     M.Fatima Bonaldo. RNA from normal foreskin melanocytes
                     (FS374) was kindly provided by Dr. Anthony P. Albino."
BASE COUNT          163 a           71 c           80 g          142 t
ORIGIN      
        1 acatcaaaat gtttattttt tggatataga catttaaaac attcatctcc aagaacagct
       61 tcaatcaggt atacaataag aaatagactt taaatcctaa tacagaaaag gtaacagaat
      121 tttttgaatg ctgctgtaga cactagtgtt agaaaaaaaa gtttataaat ggttttgcac
      181 caaaaaaata tgcaaagaaa cttgaaaata gaaattgttt gccatttgtt gctctggttg
      241 ctgtttaata tagacccagt gactgattgt cttcagcaca catctaattt ggccatttac
      301 tgcatttggc taatgtgatc caaaatgtga tctagatagt gcttttcact ggaggattga
      361 caaacacgct acatacatta gtgaatcttc ataccacctc agtgaggtag cttagaaata
      421 ttatacagaa attgaggcnc aagaggtaag tgacttg
//