LOCUS MF048860 5179 bp DNA linear SYN 30-MAY-2017
DEFINITION Synthetic construct (code 12) clone p416-HAH2-MET3, complete
sequence.
ACCESSION MF048860
VERSION MF048860.1
KEYWORDS .
SOURCE synthetic construct (code 12)
ORGANISM synthetic construct (code 12)
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 5179)
AUTHORS Bijlani,S., Rawal,Y., Malik,S., Kaur,H. and Ganesan,K.
TITLE Single-Transformation Gene Function Test by Conditional Expression
of Both Alleles of Candida albicans Genes
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5179)
AUTHORS Bijlani,S., Rawal,Y., Malik,S., Kaur,H. and Ganesan,K.
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2017) Yeast Molecular Biology, CSIR- Institute of
Microbial Technology, Sector 39-A, Chandigarh, Chandigarh 160036,
India
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5179
/organism="synthetic construct (code 12)"
/mol_type="other DNA"
/db_xref="taxon:2005482"
/clone="p416-HAH2-MET3"
misc_feature 22..55
/note="LoxLE"
gene 60..928
/gene="HIS1"
/note="5' region; derived from Candida albicans HIS1"
repeat_region 575..928
/gene="HIS1"
/rpt_type=direct
gene 934..2884
/gene="ARG4"
/note="derived from Candida albicans ARG4"
CDS 1391..2797
/gene="ARG4"
/codon_start=1
/transl_table=12
/product="argininosuccinate lyase"
/protein_id="ART66072.1"
/translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDAD
LTGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRL
GEIIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDV
LMPGYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGI
DREFLAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFG
FIKLADAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQE
DKEPLFDALTTVEHSILIATGVISTLSIDKQNMEKALTMDMLATDLADYLVRKGVPFR
ETHHISGECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGG
TAKSAVLKQLENLKSILS"
gene 2889..3738
/gene="HIS1"
/note="3' region; derived from Candida albicans HIS1"
repeat_region 2889..3242
/gene="HIS1"
/rpt_type=direct
misc_feature 3739..3772
/note="LoxRE"
regulatory 3781..5176
/regulatory_class="promoter"
/note="CaMET3 promoter; derived from Candida albicans"
BASE COUNT 1718 a 820 c 910 g 1731 t
ORIGIN
1 cttcgtacgc tgcaggtcga ctaccgttcg tataatgtat gctatacgaa gttatggatc
61 ctggaggatg aggagacaga agttagtagt aacaatttgg ggacagaaga ggatacagtg
121 acatcagagg aagaagacag cgaaggtata gtcaaaaagc aataggacag caagacaatt
181 tatttattta atagtttttt ataaatgtaa aatctatcac aacatgtgta cttaattttc
241 tgtggtaggg gaggccattt cgttgaaacg tgtcgcgagc ggttgtgaga attgactcat
301 tttgattatt acgcaaccat tgaaattttt tatttttttt tggtgaagat ttttcccaca
361 caacttcttc ttttacttaa ccaccaacta ccgataatgg atttagtcaa tcatttacca
421 gaccgtttgt tatttgctgt tcctaaaaag ggcagattat acgaaaaatg ctgtaactta
481 ttgagtggtg ccgatataca gtttagaaga tctaatagat tagatatagc actttctaca
541 aacttgccaa ttgcattaat cttcttgcct gcagctgata tcccagtttt cgttggagaa
601 ggtaattgtg acttgggtat aactgggtta gaccaaatca aagaagctga acaattcgac
661 aacatcgagg acttgttgga tttgaaattt ggttcatgca aattgcagat ccaagttcca
721 gcagatggcg agtacgaaaa gccagaacag cttgttggaa agaaaattgt gtcttcattt
781 acaaaattga gtaccgacta tttcaaacaa ttgtcagaca aacctactaa tatcagatat
841 gtcggtggtt ccgttgaggc ttcttgtgcc ttgggtgttg ctgatgctat tgtcgatttg
901 gttgaaagtg gtgaaactat gaaagcagga tccccccttt agtaagattt ttcaagagta
961 gtctcaaata aacagaatga aggattgaag gttttattca ataaataatt ggacagaaag
1021 ttattgcaca gttttaacaa caacaatttc aaaatttcaa tttatgacat attgaccgac
1081 ataattcagt catttctaat attcaaataa caagacacct tgtataaatg aatccacaag
1141 cgtatatgaa caaccaaatt ttatgactaa ctgtgggtcg tgtacttttg acgattgagt
1201 catttttacc aattaggtaa ttgaatttta ctaatttgtc attaaccgcg ctggccatcc
1261 caacaacaac aacaacaatc aatttaaaaa cttgacaagg caaaaaaaaa aaatttcatc
1321 tttgaaaaat ttttcttcct tttcttcttc tgttctttac ataacagcta tcaagaataa
1381 tcaattaata atgtcacaac aacaagataa acaacctagt gaaaataaat tatggggtgg
1441 tcgtttcacc ggtgccactg atccattgat ggatttgtat aatgcttcat taccttatga
1501 taaagttatg tatgatgctg atttaactgg tactaaagta tacacacaag gattaaacaa
1561 attaggatta attaccactg aagaattgaa tttgattcat caaggattag aacaaatcag
1621 acaagaatgg catgataata aattcattat taaggctggt gatgaggata ttcatactgc
1681 taatgaacgt cgattaggag aaatcattgg gaaaaacatt tctggtaaag ttcacactgg
1741 tagatcaaga aatgatcaag ttgctaccga tatgagaatt ttcgttcgtg aatcattact
1801 taatttaagt aaaattttac atcaattcat cacggcaatt cttgaacgag cacataaaga
1861 aatcgatgta ttaatgcctg gttatactca tttacaaaaa gctcaaccta ttagatgggc
1921 ccattggtta agttcatatg caacttattt caccgaagat tataaacgtt tacaagaaat
1981 tataactcgt gttaaccaat ctccattagg atctggtgct ttagcaggtc atccttatgg
2041 tattgatcgt gaatttttag ctaaaggatt aggatttgat ggtgtcattg ggaattcttt
2101 aactgcagtt tcagatcgtg attttgttgt tgaaagttta ttttggagta cattatttat
2161 gaatcatatt tctcgattct cagaagatct aattatttat tctagtggtg aatttgggtt
2221 cataaaatta gctgatgctt attctactgg ttcttcattg atgccacaaa agaaaaaccc
2281 tgactcatta gaattattac gtgggaaaag tggtagagta tttggtcagt tatctggatt
2341 tttaatgtcg atcaaatcta ttccatctac ttataataaa gatatgcaag aggataaaga
2401 accattattt gatgcattga ctacagtaga acattcaatt ttaattgcta ctggtgtgat
2461 ttctacttta ctgattgata aacaaaatat ggaaaaagct ttaactatgg atatgttggc
2521 tactgattta gctgattatt tagttagaaa aggagttcca tttagagaaa ctcatcatat
2581 atctggtgaa tgtgttagaa aagctgaaga agaaaaatta tctggaattg atcaattatc
2641 ttttgaacaa tttcaacaaa ttgattctcg ttttgaaaaa gacgttatgg aaacttttga
2701 ttttgaagct agtgtggaaa gaagagatgc tcttggtggt actgctaaaa gtgccgtttt
2761 aaaacaatta gaaaatttaa aatcaatctt aagttaattt ataaatagtc atataataat
2821 cacagtattg tttaacttac ataaaatata atcaattaac acagagatac cttgtactac
2881 aatagtttct gatatcccag ttttcgttgg agaaggtaat tgtgacttgg gtataactgg
2941 gttagaccaa atcaaagaag ctgaacaatt cgacaacatc gaggacttgt tggatttgaa
3001 atttggttca tgcaaattgc agatccaagt tccagcagat ggcgagtacg aaaagccaga
3061 acagcttgtt ggaaagaaaa ttgtgtcttc atttacaaaa ttgagtaccg actatttcaa
3121 acaattgtca gacaaaccta ctaatatcag atatgtcggt ggttccgttg aggcttcttg
3181 tgccttgggt gttgctgatg ctattgtcga tttggttgaa agtggtgaaa ctatgaaagc
3241 agctgggtta aaggccatag aaaccatatt ggagacttcg gctcatttga tttcgtcaaa
3301 aaaaagtaaa ttcccagaaa tggtcaatat aatcgtccaa agacttcaag gtgttttagc
3361 tgcccaggaa tatgtcttgt gtaactacaa tgccccaaaa tccatccaag caaaatgttt
3421 aaccattact ccaggcagaa gggccgctac tgtctctact ttggacaaac acagcgacga
3481 cgaagaggac tgggttgcca tctcatccat ggttaataga aaggaaatcg gtaatgtaat
3541 ggacgaattg aagaaagctg gtgcaaccga tatattggta ctcgagatat caaattgtag
3601 agtttaaaag aagtgatagt ttctcataaa tattatacat atatacttat agatctactt
3661 caatttgact caatctgtct tataccatct ttatctacca ccttgatgta cacaccttta
3721 aagtcaatag gcattctcat aacttcgtat aatgtatgct atacgaacgg tagtttaaac
3781 aatgactatt ggtagcgtgt tctatttttc ttatgcgctg aaaaactacg aacaattgtc
3841 tattccaagc ctgtggcttt ttttggtgat tcacaataca taaattggca aatacctctt
3901 ctatagactt taacctttga tgaaatggta tggataaacc acttttgtgg tcagggtata
3961 tatattttta ttccaatctg gcctttataa attcaaattt ccaaggggac tctgctaaac
4021 cttctgatat aactataact ctaataatgg ctgtaaaaag tgtgaaaaaa agatgatatt
4081 cacaaaaaag ataaagggga tttcatattg ggtatctaga tttaaacatc gtatttcaac
4141 aatgaaacta gaatcaatac gaaatccagt gcacaactgt gttcattccc ttgggaatta
4201 tactgagtaa tgggaagaat gtgcaaaaaa tacccgtgtc ttatataatt tccatttgtg
4261 tagggcttaa aagtggacaa cttcaggtta cgaaaccacg aaaatgtcta aacgtttgat
4321 caaattcgta actcatggaa aatcacagca acaaattaag gatggatata tccggtactt
4381 acaaataaaa acactaaaga agtataaatt ttgaactgaa attctataga aaatcttttt
4441 tttttgggta aaattatgcg attgtggctc atagtaacgt aatcatacag ttcaacaaac
4501 atcattttgt caaaggattt ctaatcccat tctttttcta cttggctttt gagtatatat
4561 atatatacat cctttgttca tcatcatgtt cgattgaatt tttttcttgg ttgttattag
4621 attgtctact tgagtttttc ttggtgcaaa ttgtggtgtt tttctaacaa atttttcaac
4681 atttaaagta tttctggaac ttttctacaa ggaacgacat agatccatta atgcgcccct
4741 ctaaaacaat acccataatg tcttcttaag tatgcatctg cttataaatg tttctggatt
4801 tactttagtt agaatgtcga cacgacaatg aactattgct gtggatcacg tgcgatcact
4861 gaattatgag tttcttttta tttttctttt tgggcttttt tttgcaattt tttttttcca
4921 cctttgctaa ttgcgtagtc acatataatg tatacaattt ttgcaactct gacactataa
4981 ttgatccaat aataaagtat ataatagatc atcaagtata cgtaatctcc ccaaaaaaaa
5041 aattgcataa aataaaatct ataaaccttt tttagatttt tagttgttcc ttttttcttt
5101 ctattcttca acttccaagt gttgtcactt tcttcctgtt aactatattt aaaagtaaat
5161 accctcccca gaaaacatg
//