LOCUS       HUMNKSFP40              2318 bp    mRNA    linear   HUM 07-JAN-1995
DEFINITION  Human natural killer cell stimulatory factor (NKSF) mRNA, complete
            cds, clone p40.
ACCESSION   M65290
VERSION     M65290.1
KEYWORDS    cytokine; natural killer cell stimulatory factor.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Wolf,S.F., Temple,P.A., Kobayashi,M., Young,D., Dicig,M., Lowe,L.,
            Dzialo,R., Fitz,L., Ferenz,C., Hewick,R.M., Kelleher,K.,
            Herrmann,S.H., Clark,S.C., Azzoni,L., Chan,S.H., Trinchieri,G. and
            Perussia,B.
  TITLE     Cloning of cDNA for natural killer cell stimulatory factor, a
            heterodimeric cytokine with multiple biologic effects on T and
            natural killer cells
  JOURNAL   J. Immunol. 146 (9), 3074-3081 (1991)
   PUBMED   1673147
COMMENT     Original source text: Human EBV-transformed B cell line RPMI 8866,
            cDNA to mRNA, clone p40.
FEATURES             Location/Qualifiers
     source          1..2318
                     /db_xref="H-InvDB:HIT000196178"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /map="Unassigned"
                     /cell_line="EBV-transformed B cell line RPMI 8866"
     gene            1..2318
                     /gene="NKSF2"
     CDS             14..1000
                     /gene="NKSF2"
                     /codon_start=1
                     /product="natural killer cell stimulatory factor"
                     /protein_id="AAA59938.1"
                     /db_xref="GI:189236"
                     /db_xref="GDB:G00-127-870"
                     /translation="MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGE
                     MVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLL
                     LLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSR
                     GSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKL
                     KYENYTSSFFIRDIIKPDPPNNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQ
                     VQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS"
     sig_peptide     14..79
                     /gene="NKSF2"
                     /note="G00-127-870"
     mat_peptide     80..997
                     /gene="NKSF2"
                     /product="natural killer cell stimulatory factor"
                     /note="G00-127-870"
BASE COUNT          692 a          478 c          535 g          613 t
ORIGIN      
        1 ggcccagagc aagatgtgtc accagcagtt ggtcatctct tggttttccc tggtttttct
       61 ggcatctccc ctcgtggcca tatgggaact gaagaaagat gtttatgtcg tagaattgga
      121 ttggtatccg gatgcccctg gagaaatggt ggtcctcacc tgtgacaccc ctgaagaaga
      181 tggtatcacc tggaccttgg accagagcag tgaggtctta ggctctggca aaaccctgac
      241 catccaagtc aaagagtttg gagatgctgg ccagtacacc tgtcacaaag gaggcgaggt
      301 tctaagccat tcgctcctgc tgcttcacaa aaaggaagat ggaatttggt ccactgatat
      361 tttaaaggac cagaaagaac ccaaaaataa gacctttcta agatgcgagg ccaagaatta
      421 ttctggacgt ttcacctgct ggtggctgac gacaatcagt actgatttga cattcagtgt
      481 caaaagcagc agaggctctt ctgaccccca aggggtgacg tgcggagctg ctacactctc
      541 tgcagagaga gtcagagggg acaacaagga gtatgagtac tcagtggagt gccaggagga
      601 cagtgcctgc ccagctgctg aggagagtct gcccattgag gtcatggtgg atgccgttca
      661 caagctcaag tatgaaaact acaccagcag cttcttcatc agggacatca tcaaacctga
      721 cccacccaac aacttgcagc tgaagccatt aaagaattct cggcaggtgg aggtcagctg
      781 ggagtaccct gacacctgga gtactccaca ttcctacttc tccctgacat tctgcgttca
      841 ggtccagggc aagagcaaga gagaaaagaa agatagagtc ttcaccgaca agacctcagc
      901 cacggtcatc tgccgcaaaa atgccagcat tagcgtgcgg gcccaggacc gctactatag
      961 ctcatcttgg agcgaatggg catctgtgcc ctgcagttag gttctgatcc aggatgaaaa
     1021 tttggaggaa aagtggaaga tattaagcaa aatgtttaaa gacacaacgg aatagaccca
     1081 aaaagataat ttctatctga tttgctttaa aacgtttttt taggatcaca atgatatctt
     1141 tgctgtattt gtatagttcg atgctaaatg ctcattgaaa caatcagcta atttatgtat
     1201 agattttcca gctctcaagt tgccatgggc cttcatgcta tttaaatatt taagtaattt
     1261 atgtatttat tagtatatta ctgttattta acgtttgtct gccaggatgt atggaatgtt
     1321 tcatactctt atgacctgat ccatcaggat cagtccctat tatgcaaaat gtgaatttaa
     1381 ttttatttgt actgacaact tttcaagcaa ggctgcaagt acatcagttt tatgacaatc
     1441 aggaagaatg cagtgttctg ataccagtgc catcatacac ttgtgatgga tgggaacgca
     1501 agagatactt acatggaaac ctgacaatgc aaacctgttg agaagatcca ggagaacaag
     1561 atgctagttc ccatgtctgt gaagacttcc tggagatggt gttgataaag caatttaggg
     1621 ccacttacac ttctaagcaa gtttaatctt tggatgcctg aattttaaaa gggctagaaa
     1681 aaaatgattg accagcctgg gaaacataac aagaccccgt ctctacaaaa aaaatttaaa
     1741 attagccagg cgtggtggct catgcttgtg gtcccagctg ttcaggagga tgaggcagga
     1801 ggatctcttg agcccaggag gtcaaggcta tggtgagccg tgattgtgcc actgcatacc
     1861 agcctaggtg acagaatgag accctgtctc aaaaaaaaaa atgattgaaa ttaaaattca
     1921 gctttagctt ccatggcagt cctcaccccc acctctctaa aagacacagg aggatgacac
     1981 agaaacaccg taagtgtctg gaaggcaaaa agatcttaag attcaagaga gaggacaagt
     2041 agttatggct aaggacatga aattgtcaga atggcaggtg gcttcttaac agccatgtga
     2101 gaagcagaca gatgcaaaga aaatctggaa tccctttctc attagcatga atgaacctga
     2161 tacacaatta tgaccagaaa atatggctcc atgaaggtgc tacttttaag taatgtatgt
     2221 gcgctctgta aagtgattac atttgtttcc tgtttgttta tttatttatt tatttttgca
     2281 ttctgaggct gaactaataa aaactcttct ttgtaatc
//