LOCUS BACMDH 2397 bp DNA linear BCT 02-DEC-2016
DEFINITION Bacillus sp. (in: Bacteria) methanol dehydrogenase alpha-10 subunit
(mdh) gene, complete cds.
ACCESSION M65004
VERSION M65004.2
KEYWORDS .
SOURCE Bacillus sp. (in: Bacteria)
ORGANISM Bacillus sp. (in: Bacteria)
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE 1 (bases 1 to 2397)
AUTHORS de Vries,G.E., Arfman,N., Terpstra,P. and Dijkhuizen,L.
TITLE Cloning, expression, and sequence analysis of the Bacillus
methanolicus C1 methanol dehydrogenase gene
JOURNAL J. Bacteriol. 174 (16), 5346-5353 (1992)
PUBMED 1644761
COMMENT On Dec 2, 2016 this sequence version replaced M65004.1.
FEATURES Location/Qualifiers
source 1..2397
/organism="Bacillus sp. (in: Bacteria)"
/mol_type="genomic DNA"
/strain="C1"
/db_xref="taxon:1409"
gene 661..1921
/gene="mdh"
regulatory 661..690
/regulatory_class="promoter"
/gene="mdh"
/note="putative"
CDS 729..1874
/gene="mdh"
/EC_number="1.1.1.1"
/function="methylotrophy"
/note="putative"
/codon_start=1
/transl_table=11
/product="methanol dehydrogenase alpha-10 subunit"
/protein_id="AAA22593.1"
/translation="MTNFFIPPASVIGRGAVKEVGTRLKQIGAKKALIVTDAFLHSTG
LSEEVAKNIREAGLDVAIFPKAQPDPADTQVHEGVDVFKQENCDALVSIGGGSSHDTA
KAIGLVAANGGRINDYQGVNSVEKPVVPVVAITTTAGTGSETTSLAVITDSARKVKMP
VIDEKITPTVAIVDPELMVKKPAGLTIATGMDALSHAIEAYVAKGATPVTDAFAIQAM
KLINEYLPKAVANGEDIEAREAMAYAQYMAGVAFNNGGLGLVHSISHQVGGVYKLQHG
ICNSVNMPHVCAFNLIAKTERFAHIAELLGENVSGLSTAAAAERAIVALERYNKNFGI
PSGYAEMGVKEEDIELLAKNAFEDVCTQSNPRVATVQDIAQIIKNAL"
protein_bind 762..839
/gene="mdh"
/note="putative"
/bound_moiety="nicotinamide-adenine dinucleotide (NAD)"
regulatory 1885..1921
/regulatory_class="terminator"
/gene="mdh"
BASE COUNT 851 a 429 c 451 g 666 t
ORIGIN
1 ggatccgtcc ataccattct gagtcggatg atgaataaga atgctgtcat cgttttcttt
61 tatggaaatg tcgggtacat atacattaaa aaagaccgcc gatgatacaa caccaaacgg
121 ttatgtaata aaaggttccc ttaaaagagg gatatcagct aaccaaagaa aagtaaatcc
181 agacctttaa cgcttaccgg ctcaaggatg gatttacttt ttttatgtta aagcaatata
241 tcgatctagc ttaaaaacga aatagcgaac atcaatgctt gaaaaacggt cagaagccgc
301 tctctctttc gggaatactg ctaccgttga gtttatctat atttattgcc tattctatta
361 ctcttttatt ataaaagctt caagttgacc acaaaaaaac tttcattttt taagacaatt
421 tgatgcctta ggatctagtc gatcagtcgt tttatgatat aactttaatc ctttttacaa
481 agtcaaattt aaatcctgcc cttccacttt aatcctccac actatataag cgtcaaaatc
541 actttttctt tggaaatctt ttattaggtt gtttttccct tgagcttttt atataaaatt
601 aatattcaga tccttctaaa aaatataatt tcgaaaacta agaacattac gacaactata
661 ttgacaaaca tttagattag catttaaact agttttgtaa acaattacat aaataggagg
721 tagtaagaat gacaaacttt ttcattccac cagccagcgt aattggacga ggtgcagtaa
781 aggaagtagg aacaagactt aagcaaattg gagctaagaa agcgcttatc gttacagatg
841 catttcttca tagcacaggt ttatctgaag aagttgctaa aaacattcgt gaagctggcc
901 ttgatgttgc gattttccca aaagctcaac cagatccagc agatacacaa gttcatgaag
961 gtgtagatgt attcaaacaa gaaaactgtg atgcacttgt ttctatcggt ggaggtagct
1021 ctcacgatac agctaaagca atcggtttag ttgcagcaaa cggcggaaga atcaatgact
1081 atcaaggtgt aaacagtgta gaaaaaccag tcgttccagt agttgcaatc actacaacag
1141 ctggtactgg tagtgaaaca acatctcttg cagttattac agactctgca cgtaaagtaa
1201 aaatgcctgt tattgatgag aaaattactc caactgtagc aattgttgac ccagaattaa
1261 tggtgaaaaa accagctgga ttaacaatcg caactggtat ggacgcatta tcacacgcaa
1321 ttgaagcata tgttgcaaaa ggtgctacac cagttactga tgcatttgca attcaagcaa
1381 tgaaactcat caatgaatac ttaccaaaag cggtggcaaa cggagaagac atcgaagcac
1441 gtgaagcaat ggcttatgca caatacatgg caggagtggc atttaacaac ggtggtttag
1501 gattagtaca ctctatttct caccaagtag gtggagttta caaattacaa cacggaatct
1561 gtaactcagt taatatgcca cacgtttgcg cattcaacct aattgctaaa actgagcgct
1621 tcgcacacat tgctgagctt ttaggcgaga atgtttctgg cttaagcact gcagcagctg
1681 ctgagagagc aattgtagcg cttgaacgct ataacaaaaa cttcggtatc ccatctggct
1741 atgcagaaat gggcgtgaaa gaagaggata tcgaattatt agcgaaaaac gcattcgaag
1801 acgtatgtac tcaaagcaac ccacgtgttg ctacagttca agacattgca caaatcatca
1861 aaaacgctct gtaatcataa tcttggaaaa gcgtctcttg aaattcaaga gacgcttttc
1921 cgtgatgcta gattaaatag tagacacaga ttaatagagt aatagactaa agaatgagga
1981 gactacatgt cgaaaaacaa aggcggtacg aggaagattt taaaaaatat atcgaatccc
2041 gccaactgta taggagttca aaagttatga aaaagctcaa tcatgaagga attgatgttt
2101 ctcagaagac ggtccaatga aagatgaagg aatgaagtct cgtaccgtaa aaaaataaaa
2161 ccacacggtc catgaaaatg tcttaaacaa taaagtgtgg gtgaatgagg aaaaactaca
2221 tggaaatcgg tcttgaaaac gaaataaaaa ttagaaaaac tctctctcgt ttaatacagt
2281 atactattcg atactatatg ttaataaagt gcatacttac gaattgatat tatacacgtt
2341 atactagatt ttgtataggt aatatcgaat atagaaagtc ataaataaca caggagg
//