LOCUS       HUMIF4E                 1842 bp    mRNA    linear   HUM 01-SEP-1995
DEFINITION  Homo sapiens cap-binding protein mRNA, complete cds.
ACCESSION   M15353
VERSION     M15353.1
KEYWORDS    cap-binding protein; eIF-4E gene.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Rychlik,W., Domier,L.L., Gardner,P.R., Hellmann,G.M. and
            Rhoads,R.E.
  TITLE     Amino acid sequence of the mRNA cap-binding protein from human
            tissues
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 84 (4), 945-949 (1987)
   PUBMED   3469651
COMMENT     On Jul 26, 1993 this sequence version replaced gi:179973.
            Original source text: Homo sapiens (clone library: library of J.
            Whittaker) cDNA to mRNA.
            Draft entry and computer-readable sequence for [1] kindly provided
            by L.Domier, 21-APR-1987.
            There are at least two species of CBP mRNA of about 1900 and 2500
            nucleotides each.  There may be alternative start sites.  Potential
            polyadenylation signals are located at positions 1315-1320,
            1621-1626, and 1811-1816.
FEATURES             Location/Qualifiers
     source          1..1842
                     /db_xref="H-InvDB:HIT000194390"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /cell_line="IM9"
                     /cell_type="lymphocyte"
                     /clone_lib="library of J. Whittaker"
     mRNA            <1..1842
                     /product="CBP mRNA"
     CDS             19..672
                     /codon_start=1
                     /product="cap-binding protein"
                     /protein_id="AAC13647.1"
                     /db_xref="GI:306487"
                     /translation="MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWA
                     LWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMW
                     EDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKG
                     DKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV"
     regulatory      1314..1319
                     /regulatory_class="polyA_signal_sequence"
     regulatory      1620..1625
                     /regulatory_class="polyA_signal_sequence"
     regulatory      1810..1815
                     /regulatory_class="polyA_signal_sequence"
     polyA_site      1842
BASE COUNT          550 a          310 c          338 g          644 t
ORIGIN      
        1 cgatcagatc gatctaagat ggcgactgtc gaaccggaaa ccacccctac tcctaatccc
       61 ccgactacag aagaggagaa aacggaatct aatcaggagg ttgctaaccc agaacactat
      121 attaaacatc ccctacagaa cagatgggca ctctggtttt ttaaaaatga taaaagcaaa
      181 acttggcaag caaacctgcg gctgatctcc aagtttgata ctgttgaaga cttttgggct
      241 ctgtacaacc atatccagtt gtctagtaat ttaatgcctg gctgtgacta ctcacttttt
      301 aaggatggta ttgagcctat gtgggaagat gagaaaaaca aacggggagg acgatggcta
      361 attacattga acaaacagca gagacgaagt gacctcgatc gcttttggct agagacactt
      421 ctgtgcctta ttggagaatc ttttgatgac tacagtgatg atgtatgtgg cgctgttgtt
      481 aatgttagag ctaaaggtga taagatagca atatggacta ctgaatgtga aaacagagaa
      541 gctgttacac atatagggag ggtatacaag gaaaggttag gacttcctcc aaagatagtg
      601 attggttatc agtcccacgc agacacagct actaagagcg gctccaccac taaaaatagg
      661 tttgttgttt aagaagacac cttctgagta ttctcatagg agactgcgtc aagcaatcga
      721 gatttgggag ctgaaccaaa gcctcttcaa aaagcagagt ggactgcatt taaatttgat
      781 ttccatctta atgttactca gatataagag aagtctcatt cgcctttgtc ttgtacttct
      841 gtgttcattt tttttttttt tttttggcta gagtttccac tatcccaatc aaagaattac
      901 agtacacatc cccagaatcc ataaatgtgt tcctggccca ctctgtaata gttcagtaga
      961 attaccatta attacataca gattttacct atccacaata gtcagaaaac aacttggcat
     1021 ttctatactt tacaggaaaa aaaattctgt tgttccattt tatgcagaag catattttgc
     1081 tggtttgaaa gattatgatg catacagttt tctagcaatt ttctttgttt ctttttacag
     1141 cattgtcttt gctgtactct tgctgatggc tgctagattt taatttattt gtttccctac
     1201 ttgataatat tagtgattct gatttcagtt tttcatttgt tttgcttaaa tttttttttt
     1261 ttttttcctc atgtaacatt ggtgaaggat ccaggaatat gacacaaagg tggaataaac
     1321 attaattttg tgcattcttt ggtaattttt tttgtttttt gtaactacaa agctttgcta
     1381 caaatttatg catttcattc aaatcagtga tctatgtttg tgtgatttcc taaacataat
     1441 tgtggattat aaaaaatgta acatcataat tacattccta actagaatta gtatgtctgt
     1501 ttttgtatct ttatgctgta ttttaacact ttgtattact taggttattt tgctttggtt
     1561 aaaaatggct caagtagaaa agcagtccca ttcatattaa gacagtgtac aaaactgtaa
     1621 ataaaatgtg tacagtgaat tgtcttttag acaactagat ttgtcctttt atttctccat
     1681 ctttatagaa ggaatttgta cttcttattg caggcaagtc tctatattat gtcctctttt
     1741 gtggtgtctt ccatgtgaac agcataagtt tggagcacta gtttgattat tatgtttatt
     1801 acaattttta ataaattgaa taggtagtat catatatatg ga
//