LOCUS HUMFVII 2462 bp mRNA linear HUM 13-FEB-1996
DEFINITION Human factor VII serine protease precursor mRNA, complete cds,
clone lambda-HVII2463.
ACCESSION M13232
VERSION M13232.1
KEYWORDS factor VII; serine protease; serum glycoprotein.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2462)
AUTHORS Hagen,F.S., Gray,C.L., O'Hara,P.J., Grant,F.J., Saari,G.C.,
Woodbury,R.G., Hart,C.E., Insley,M., Kisiel,W., Kurachi,K. and
Davie,E.W.
TITLE Characterization of a cDNA coding for human factor VII
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 83 (8), 2412-2416 (1986)
PUBMED 3486420
COMMENT Original source text: Homo sapiens liver cDNA to mRNA.
Draft entry and sequence in computer-readable form for [1] kindly
provided by F.S.Hagen.
[1] sequenced two alternatively spliced mRNAs that produced
shortened signal peptides. One is presented as factor VIIb below.
FEATURES Location/Qualifiers
source 1..2462
/db_xref="H-InvDB:HIT000194212"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/tissue_type="liver"
mRNA <1..2462
/product="FVIIa mRNA"
CDS 36..1436
/note="precursor for factor VIIa and b"
/codon_start=1
/product="coagulation factor VII"
/protein_id="AAA88040.1"
/translation="MVSQALRLLCLLLGLQGCLAAGGVAKASGGETRDMPWKPGPHRV
FVTQEEAHGVLHRRRRANAFLEELRPGSLERECKEEQCSFEEAREIFKDAERTKLFWI
SYSDGDQCASSPCQNGGSCKDQLQSYICFCLPAFEGRNCETHKDDQLICVNENGGCEQ
YCSDHTGTKRSCRCHEGYSLLADGVSCTPTVEYPCGKIPILEKRNASKPQGRIVGGKV
CPKGECPWQVLLLVNGAQLCGGTLINTIWVVSAAHCFDKIKNWRNLIAVLGEHDLSEH
DGDEQSRRVAQVIIPSTYVPGTTNHDIALLRLHQPVVLTDHVVPLCLPERTFSERTLA
FVRFSLVSGWGQLLDRGATALELMVLNVPRLMTQDCLQQSRKVGDSPNITEYMFCAGY
SDGSKDSCKGDSGGPHATHYRGTWYLTGIVSWGQGCATVGHFGVYTRVSQYIEWLQKL
MRSEPRPGVLLRAPFP"
sig_peptide 36..215
/note="factor VIIa signal peptide"
CDS join(36..99,166..1436)
/note="preprofactor VIIb"
/codon_start=1
/protein_id="AAA88041.1"
/translation="MVSQALRLLCLLLGLQGCLAAVFVTQEEAHGVLHRRRRANAFLE
ELRPGSLERECKEEQCSFEEAREIFKDAERTKLFWISYSDGDQCASSPCQNGGSCKDQ
LQSYICFCLPAFEGRNCETHKDDQLICVNENGGCEQYCSDHTGTKRSCRCHEGYSLLA
DGVSCTPTVEYPCGKIPILEKRNASKPQGRIVGGKVCPKGECPWQVLLLVNGAQLCGG
TLINTIWVVSAAHCFDKIKNWRNLIAVLGEHDLSEHDGDEQSRRVAQVIIPSTYVPGT
TNHDIALLRLHQPVVLTDHVVPLCLPERTFSERTLAFVRFSLVSGWGQLLDRGATALE
LMVLNVPRLMTQDCLQQSRKVGDSPNITEYMFCAGYSDGSKDSCKGDSGGPHATHYRG
TWYLTGIVSWGQGCATVGHFGVYTRVSQYIEWLQKLMRSEPRPGVLLRAPFP"
sig_peptide join(36..99,166..215)
/note="factor VIIb signal peptide"
mat_peptide 216..671
/product="coagulation factor VII"
/note="light chain"
mat_peptide 672..1433
/product="coagulation factor VII"
/note="heavy chain"
exon <36..99
/note="preprofactor VIIb"
/number=1
exon 100..165
/note="alternate exon; putative"
exon 166..2462
/note="factor VIIb"
/number=2
BASE COUNT 590 a 724 c 721 g 427 t
ORIGIN 24 bp upstream of PstI site.
1 tcaacaggca ggggcagcac tgcagagatt tcatcatggt ctcccaggcc ctcaggctcc
61 tctgccttct gcttgggctt cagggctgcc tggctgcagg cggggtcgct aaggcctcag
121 gaggagaaac acgggacatg ccgtggaagc cggggcctca cagagtcttc gtaacccagg
181 aggaagccca cggcgtcctg caccggcgcc ggcgcgccaa cgcgttcctg gaggagctgc
241 ggccgggctc cctggagagg gagtgcaagg aggagcagtg ctccttcgag gaggcccggg
301 agatcttcaa ggacgcggag aggacgaagc tgttctggat ttcttacagt gatggggacc
361 agtgtgcctc aagtccatgc cagaatgggg gctcctgcaa ggaccagctc cagtcctata
421 tctgcttctg cctccctgcc ttcgagggcc ggaactgtga gacgcacaag gatgaccagc
481 tgatctgtgt gaacgagaac ggcggctgtg agcagtactg cagtgaccac acgggcacca
541 agcgctcctg tcggtgccac gaggggtact ctctgctggc agacggggtg tcctgcacac
601 ccacagttga atatccatgt ggaaaaatac ctattctaga aaaaagaaat gccagcaaac
661 cccaaggccg aattgtgggg ggcaaggtgt gccccaaagg ggagtgtcca tggcaggtcc
721 tgttgttggt gaatggagct cagttgtgtg gggggaccct gatcaacacc atctgggtgg
781 tctccgcggc ccactgtttc gacaaaatca agaactggag gaacctgatc gcggtgctgg
841 gcgagcacga cctcagcgag cacgacgggg atgagcagag ccggcgggtg gcgcaggtca
901 tcatccccag cacgtacgtc ccgggcacca ccaaccacga catcgcgctg ctccgcctgc
961 accagcccgt ggtcctcact gaccatgtgg tgcccctctg cctgcccgaa cggacgttct
1021 ctgagaggac gctggccttc gtgcgcttct cattggtcag cggctggggc cagctgctgg
1081 accgtggcgc cacggccctg gagctcatgg tgctcaacgt gccccggctg atgacccagg
1141 actgcctgca gcagtcacgg aaggtgggag actccccaaa tatcacggag tacatgttct
1201 gtgccggcta ctcggatggc agcaaggact cctgcaaggg ggacagtgga ggcccacatg
1261 ccacccacta ccggggcacg tggtacctga cgggcatcgt cagctggggc cagggctgcg
1321 caaccgtggg ccactttggg gtgtacacca gggtctccca gtacatcgag tggctgcaaa
1381 agctcatgcg ctcagagcca cgcccaggag tcctcctgcg agccccattt ccctagccca
1441 gcagccctgg cctgtggaga gaaagccaag gctgcgtcga actgtcctgg caccaaatcc
1501 catatattct tctgcagtta atggggtaga ggagggcatg ggagggaggg agaggtgggg
1561 agggagacag agacagaaac agagagagac agagacagag agagactgag ggagagactc
1621 tgaggacatg gagagagact caaagagact ccaagattca aagagactaa tagagacaca
1681 gagatggaat agaaaagatg agaggcagag gcagacaggc gctggacaga ggggcagggg
1741 agtgccaagg ttgtcctgga ggcagacagc ccagctgagc ctccttacct cccttcagcc
1801 aagccccacc tgcacgtgat ctgctggccc tcaggctgct gctctgcctt cattgctgga
1861 gacagtagag gcatgaacac acatggatgc acacacacac acgccaatgc acacacacag
1921 agatatgcac acacacggat gcacacacag atggtcacac agagatacgc aaacacaccg
1981 atgcacacgc acatagagat atgcacacac agatgcacac acagatatac acatggatgc
2041 acgcacatgc caatgcacgc acacatcagt gcacacggat gcacagagat atgcacacac
2101 cgatgtgcgc acacacagat atgcacacac atggatgagc acacacacac caagtgcgca
2161 cacacaccga tgtacacaca cagatgcaca cacagatgca cacacaccga tgctgactcc
2221 atgtgtgctg tcctctgaag gcggttgttt agctctcact tttctggttc ttatccatta
2281 tcatcttcac ttcagacaat tcagaagcat caccatgcat ggtggcgaat gcccccaaac
2341 tctcccccaa atgtatttct cccttcgctg ggtgccgggc tgcacagact attccccacc
2401 tgcttcccag cttcacaata aacggctgcg tctcctccgc acacctgtgg tgcctgccac
2461 cc
//