LOCUS       BLYAMYABA                595 bp    mRNA    linear   PLN 27-APR-1993
DEFINITION  Barley alpha-amylase type B isozyme mRNA, clone 103.
ACCESSION   M10056
VERSION     M10056.1
KEYWORDS    alpha-amylase; amylase.
SOURCE      Hordeum vulgare
  ORGANISM  Hordeum vulgare
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Huang,J.K., Swegle,M., Dandekar,A.M. and Muthukrishnan,S.
  TITLE     Expression and regulation of alpha-amylase gene family in barley
            aleurones
  JOURNAL   J. Mol. Appl. Genet. 2 (6), 579-588 (1984)
   PUBMED   6335720
COMMENT     Original source text: Barley (Hordeum vulgare cv. Himalaya)
            gibberellic acid treated aleurone cell, cDNA to mRNA, clone 103.
            Draft entry and sequence in computer-readable form kindly provided
            by S. Muthukrishnan, 03-SEP-1985 [1].
            There are at least four types of alpha-amylase.  The accumulation
            of alpha-amylase mRNAs in aleurones as a function of time after
            addition of the hormone gibberellic acid (GA) shows differential
            control of the expression of the alpha-amylase genes.  Type A
            isozyme mRNA is present in small amounts in unstimulated cells, and
            increases in concentration upon stimulation with GA, whereas type B
            isozyme mRNA is undetectable in unstimulated cells and increases a
            hundred-fold after stimulation with gibberellic acid.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Hordeum vulgare"
                     /mol_type="mRNA"
                     /db_xref="taxon:4513"
     CDS             <1..462
                     /note="alpha-amylase type B, EC 3.2.1.1"
                     /codon_start=1
                     /protein_id="AAA32930.1"
                     /translation="ILNVAVEGALWRLRGTDGKAPSMIGWWPAKAVTFVDNHDTGSTQ
                     HMWPFPSDRVMQGYAYILTHPRTPCIFYDHFFDWGPKEEIDRLVSVRTRHGIHNESKL
                     QIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFEGAAHGNDYAVWEKI"
BASE COUNT          149 a          156 c          170 g          120 t
ORIGIN      256 bp upstream of PvuI site; chromosome 6.
        1 atcctcaacg tggccgtgga gggcgcgctg tggcggctgc gcggcacaga cggtaaggcg
       61 ccaagcatga tcgggtggtg gccagccaag gcggtgacct ttgtggacaa ccacgacacc
      121 ggctccacgc agcacatgtg gcccttccct tctgacaggg tcatgcaggg atatgcctac
      181 atcctcacgc acccaaggac gccatgcatc ttctacgacc atttcttcga ctggggcccg
      241 aaggaggaga tcgatcgcct ggtgtcagtc aggacccggc acgggataca caacgagagc
      301 aagctgcaaa tcatagaggc cgacgccgac ctttatctcg ccgagatcga cggcaaggtc
      361 atcgtcaagc tcgggccaag atacgatgtg gggaacctca ttccgggagg cttcgaaggt
      421 gccgcgcacg gcaatgacta tgccgtatgg gagaaaatat gagcaaaatt gcgagagcag
      481 ctctacaagt tcctatatga tacatattag tccgagctca cgctgttcac atagtacaat
      541 taatattgct ctaatgtaaa agtgaggatg agggacatgc attgtatttt tgata
//