LOCUS LT727629 5193 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pSV23SMTNFm84V, complete sequence.
ACCESSION LT727629
VERSION LT727629.1
KEYWORDS .
SOURCE Mammalian expression vector pSV23SMTNFm84V
ORGANISM Mammalian expression vector pSV23SMTNFm84V
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5193)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5193
/organism="Mammalian expression vector pSV23SMTNFm84V"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2172."
/db_xref="taxon:1945087"
CDS <1..809
/codon_start=3
/transl_table=11
/note="part of SV40 VP1"
/protein_id="SJL89024.1"
/translation="PLPNLNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHE
NGAGKPIQGSNFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKA
VLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDEQGVGPL
CKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKNPYPISFLLSDLINR
RTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMIRYIDEFGQTTTRMQ"
regulatory complement(810..906)
/note="polyA"
/regulatory_class="terminator"
regulatory 810..906
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(907..1458)
/note="3' UTR of SV40 small t-antigen"
misc_feature complement(1459..1525)
/note="intron of SV40 small t-antigen"
CDS complement(1526..>1600)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL89025.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature complement(1608..1857)
/note="3UTR"
CDS complement(1858..>2328)
/codon_start=1
/transl_table=11
/note="mTNFm"
/protein_id="SJL89026.1"
/translation="LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLK
DNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFVISYQEKVNLLSAVKSPCPK
DTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL"
misc_feature complement(2329..2565)
/note="pmTNF"
misc_feature complement(2566..2593)
/note="5UTR"
rep_origin complement(2647..2722)
/note="SV40 ori"
regulatory complement(2679..2960)
/note="SV40 early promoter"
/regulatory_class="promoter"
misc_feature 2961..5193
/note="pAT"
rep_origin complement(3182..3584)
/note="pMB1 ori"
CDS complement(4125..4985)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL89027.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1355 a 1180 c 1291 g 1362 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgacnnn nnggaaagcc
1621 catttgagtc cttgatggtg gtgcatgaga ggcccacagt ccaggtcact gtcccagcat
1681 cttgtgtttc tgagtagttg ttgaaagctc tgagcacaga gttggactct gagccataat
1741 cccctttcta agttagaagg acacagactg ggggctctga ggagtagaca ataaaggggt
1801 cagagtaaag gggtcagagt gggggctggg tagagaatgg atgaacaccc attcccttca
1861 cagagcaatg actccaaagt agacctgccc ggactccgca aagtctaagt acttgggcag
1921 attgacctca gcgctgagtt ggtccccctt ctccagctgg aagactcctc ccaggtatat
1981 gggctcatac cagggtttga gctcagcccc ctcaggggtg tccttggggc aggggctctt
2041 gacggcagag aggaggttga ctttctcctg gtatgagatc acaaatcggc tgacggtgtg
2101 ggtgaggagc acgtagtcgg ggcagccttg tcccttgaag agaacctggg agtagacaag
2161 gtacaaccca tcggctggca ccactagttg gttgtctttg agatccatgc cgttggccag
2221 gagggcgttg gcgcgctggc tcagccactc cagctgctcc tccacttggt ggtttgctac
2281 gacgtgggct acaggcttgt cactcgaatt ttgagaagat gatctgagtg tgagggtctg
2341 ggccatagaa ctgatgagag ggaggccatt tgggaacttc tcatcccttt ggggaccgat
2401 caccccgaag ttcagtagac agaagagcgt ggtggcccct gccacaagca ggaatgagaa
2461 gaggctgaga cataggcacc gcctggagtt ctggaagccc cccatctttt gggggagtgc
2521 ctcttctgcc agttccacgt cgcggatcat gctttctgtg ctcatggtgt cttttctgga
2581 gggagatgtg gcgcgtcgac tctagagcca agcttgagct ttttgcaaaa gcctaggcct
2641 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
2701 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
2761 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
2821 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
2881 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
2941 aactgacaca cattccacag tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
3001 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
3061 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
3121 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
3181 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
3241 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
3301 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
3361 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
3421 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3481 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3541 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
3601 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
3661 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
3721 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
3781 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
3841 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
3901 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
3961 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
4021 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4081 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
4141 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
4201 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
4261 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
4321 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
4381 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
4441 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
4501 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4561 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
4621 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
4681 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
4741 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
4801 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
4861 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
4921 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
4981 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
5041 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
5101 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
5161 aggcgtatca cgaggccctt tcgtcttcaa gaa
//