LOCUS       LT727606                4607 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Vector pGEM1gHIL61, complete sequence.
ACCESSION   LT727606
VERSION     LT727606.1
KEYWORDS    .
SOURCE      Vector pGEM1gHIL61
  ORGANISM  Vector pGEM1gHIL61
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Vector pGEM1gHIL61"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 1955."
                     /db_xref="taxon:1945298"
     misc_feature    complement(25..130)
                     /note="intron"
     misc_feature    complement(131..321)
                     /note="hIL6"
     misc_feature    complement(322..483)
                     /note="intron"
     misc_feature    complement(484..502)
                     /note="hIL6"
     misc_feature    complement(503..565)
                     /note="5UTR"
     regulatory      complement(566..1101)
                     /note="hIL6"
                     /regulatory_class="promoter"
     misc_feature    complement(1102..1764)
                     /note="5FR"
     regulatory      complement(1803..1819)
                     /note="T7"
                     /regulatory_class="promoter"
     rep_origin      complement(1887..2289)
                     /note="pMB1 ori"
     CDS             complement(2830..3690)
                     /transl_table=11
                     /note="ampicillin resistance"
                     /protein_id="SJL88961.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      4585..4601
                     /note="SP6"
                     /regulatory_class="promoter"
BASE COUNT         1098 a         1183 c         1194 g         1132 t
ORIGIN      
        1 aagcttgggc tgcaggtcga ctctagaacc cagcaaagac ctcctaatgc aggcagcccc
       61 cggccacacg caggggcaag gggaacgcat gcgcaccggg cccttcaacc ccatcgccaa
      121 gcctacccac ctcctttctc agggctgaga tgccgtcgag gatgtaccga atttgtttgt
      181 caattcgttc tgaagaggtg agtggctgtc tgtgtggggc ggctacatct ttggaatctt
      241 ctcctggggg tactggggca gggaaggcag caggcaacac caggagcagc cccagggaga
      301 aggcaactgg accgaaggcg cctgtgccgg aggggagcga gcgcaggggt gagctgacag
      361 cacagctggg agcctgcctc tgctgctggc cgcccgccag ccctccctgg gccacacacc
      421 cctccctcac acagggctcg accgccgctg gcagttccag ggctaaggat ttcctgcact
      481 tacttgtgga gaaggagttc atagctgggc tcctggaggg gagatagagc ttctctttcg
      541 ttcccggtgg gctcgagggc agaatgagcc tcagacatct ccagtcctat atttattggg
      601 ggttgagact ctaatattga gactcatggg aaaatcccac atttgataaa tctttgttgg
      661 agggtgaggg tggggccaga gcgggtgggg ctgattggaa accttattaa gattgtgcaa
      721 tgtgacgtcc tttagcatcg caagacacaa ctagggggaa aagtgcagct taggtcgtca
      781 ttgaggctag cgctaagaag cagaaccact cttcctttac tttctttttt tcttttatta
      841 gtgactcagc actttggcat gtcttgacaa agagtaaagc tgaagtcatg cacgaagttt
      901 tacaaagaga aaaaaaataa gctaaagcta tgttttttga aaaaaaaaaa attttttttc
      961 agtgaccaga ttaacaggct agaatttagc gttccagtta atttgtattt gtaaaaacca
     1021 aagatgttct gaactgagtt tcctctgact ccatcgcagc cccccaagcc tgggattatg
     1081 aagaaggtaa tactaccagt catctgagtt cttctgtgtt ctggctctcc ctgtgagcgg
     1141 ctgttgtaga actgcctggc catcctcaaa tttcgtgcag ttacttcaag gcgtctccag
     1201 gtggagtgtg tgactccttt ttgccaccgc gtggcttctg ccactttctt cacctgcttc
     1261 agcccactta gaggaggttg gctgctgccc tgccctctcc caagtgaccc cttgggaatc
     1321 ctcccagtga ggactttgct ctctgtgcca gaggtttgcc ttgtgctgcc agtgctctcc
     1381 ccattgccac tgagtctctt caccactgtt tcactggggc acctgcatgg gccccaggtc
     1441 tcctggagcc ctgaaattac tgaagcccac ttggttcagg gcagaaaggg ggagaatact
     1501 actcacctct ttgttgggga tgtcaaagga ggaccttgtg gcatcttgca gagtttgtgt
     1561 ttatcgctcc ctctccctgt aagtcttgat ttgaaatcaa aagaagctag aaggaaacta
     1621 cggacgcagg cacggctcta ggctctgaat ctgcttccgc gtcggcaccc aagaatttct
     1681 tattgggtca agctggttca gaagccctct tccctcagga tggtgtctct tgcaggagga
     1741 tccttggtct catgcaaatg ggctctagag gatccccggg cgagctcgaa ttccggtctc
     1801 cctatagtga gtcgtattaa tttcgataag ccagctgcat taatgaatcg gccaacgcgc
     1861 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1921 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1981 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2041 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2101 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2161 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2221 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2281 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2341 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2401 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2461 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     2521 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2581 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2641 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2701 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2761 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2821 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2881 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2941 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3001 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3061 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3121 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3181 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3241 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3301 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3361 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3421 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3481 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3541 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3601 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3661 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3721 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3781 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     3841 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     3901 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     3961 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     4021 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatcga
     4081 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     4141 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     4201 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     4261 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     4321 gtgatgccgg ccacgatgcg tccggcgtag aggatctggc tagcgatgac cctgctgatt
     4381 ggttcgctga ccatttccgg ggtgcggaac ggcgttacca gaaactcaga aggttcgtcc
     4441 aaccaaaccg actctgacgg cagtttacga gagagatgat agggtctgct tcagtaagcc
     4501 agatgctaca caattaggct tgtacatatt gtcgttagaa cgcggctaca attaatacat
     4561 aaccttatgt atcatacaca tacgatttag gtgacactat agaatac
//