LOCUS LT727565 7117 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector AU2, complete sequence.
ACCESSION LT727565
VERSION LT727565.1
KEYWORDS .
SOURCE Vector AU2
ORGANISM Vector AU2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7117)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7117
/organism="Vector AU2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 7766."
/db_xref="taxon:1945235"
regulatory complement(26..73)
/note="T7 terminator"
/regulatory_class="terminator"
misc_feature complement(140..157)
/note="His-tag"
CDS complement(222..614)
/transl_table=11
/note="COAT"
/protein_id="SJL88853.1"
/translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYK
VTCSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDC
ELIVKAMQGLLKDGNPIPSAIAANSGIY"
CDS complement(638..1819)
/transl_table=11
/note="A"
/protein_id="SJL88854.1"
/translation="MRAFSTLDRENETFVPSVRVYADGETEDNSFSLKYRSNWTPGRF
NSTGAKTKQWHYPSPYSRGALSVTSIDQGAYKRSGSSWGRPYEEKAGFGFSLDARSCY
SLFPVSQNLTYIEVPQNVANRASTEVLQKVTQGNFNLGVALAEARSTASQLATQTIAL
VKAYTAARRGNWRQALRYLALNEDRKFRSKHVAGRWLELQFGWLPLMSDIQGAYEMLT
KVHLQEFLPMRAVRQVGTNIKLDGRLSYPAANFQTTCNISRRIVIWFYINDARLAWLS
SLGILNPLGIVWEKVPFSFVVDWLLPVGNMLEGLTAPVGCSYMSGTVTDVITGESIIS
VDAPYGWTVERQGTAKAQISAMHRGVQSVWPTTGAYVKSPFSMVHTLDALALIRQRLS
R"
misc_feature complement(1944..1988)
/note="S-tag"
misc_feature complement(1995..2012)
/note="thrombin recognition site"
misc_feature complement(2022..2039)
/note="His-tag"
misc_feature complement(2043..2087)
/note="5' UTR of T7 gene 10, incl. RBS"
regulatory complement(2089..2130)
/note="T7 promoter with lac operator"
/regulatory_class="promoter"
regulatory 2443..2520
/note="lacI promoter, incl. RBS"
/regulatory_class="promoter"
CDS 2521..3603
/transl_table=11
/note="lacI"
/protein_id="SJL88855.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
QTASPRALADSLMQLARQVSRLESGQ"
CDS 4412..4603
/transl_table=11
/note="rop"
/protein_id="SJL88856.1"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
ADELYRSCLARFGDDGENL"
rep_origin complement(4849..5251)
/note="pMB1 ori"
CDS 5743..6558
/transl_table=11
/note="Tn903 neomycin resistance"
/protein_id="SJL88857.1"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin complement(6651..7106)
/note="f1 ori"
BASE COUNT 1705 a 1823 c 1899 g 1690 t
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttc
181 ttcgacatgg gtaatcctca tgtttgaatg gccggcgtct attagtagat gccggagttt
241 gctgcgattg ctgagggaat cgggtttcca tcttttagga gaccttgcat tgccttaaca
301 ataagctcgc agtcggaatt cgtggcgaaa attggaatgg ttagttccat atttaagtac
361 gaacgccatg cggctacagg aagctctaca ccaccaacag tctgggttgc cactttaggc
421 acctcgactt tgatggtgta tttgcgattc tgcgcagagc tctgacgaac gctacaggtt
481 actttgtaag cctgtgaacg cgagttagag ctgatccatt cagcgacccc gttagcgaag
541 ttgcttgggg cgacagtcac gtcgccagtt ccgccattgt cgacgagaac gaactgagta
601 aagttagaag ccatgcttca aactccggtt gagggctcta tctagagagc cgttgcctga
661 ttaatgctaa cgcatctaag gtatggacca tcgagaaagg agactttacg tacgcgccag
721 ttgttggcca tacggattgt acccctcgat gcatggctga gatttgggcc ttagcagtgc
781 cctgtctctc cacagtccac ccgtagggag cgtcaacgct tatgatggac tcacccgtta
841 ttacgtcagt aactgttcct gacatgtagg agcatcccac gggggccgta aggccctcga
901 gcatgttacc tacaggtagg agccagtcga caacgaatga gaaaggcacc ttttcccaca
961 ctatacctag tgggttcaag atacctagag acgacaacca tgccaaacgt gcatcgttta
1021 tgtaaaacca tatcacgata cgtcgcgata tgttgcacgt tgtctggaag tttgcagctg
1081 gatacgacag acggccatct aacttgatgt tagtaccgac ctgacgtacg gctctcatag
1141 gaagaaactc ttgaaggtga accttcgtaa gcatctcata tgcaccctgg atatcactca
1201 ttagtggtaa ccaaccgaac tgcaactcca accacctgcc ggccacgtgt tttgatcgaa
1261 actttcgatc ttcgtttagg gcaaggtagc ggagcgcctg gcgccaatta ccgcgacgag
1321 cggcagtgta cgccttcacg agcgcaatgg tttgcgtcgc gagttgtgag gctgtcgacc
1381 tggcctctgc taaagcaaca ccaaggttaa aattaccctg ggtgaccttt tgcaggactt
1441 cggtcgacgc ccggttcgca acgttctgcg gcacttcgat gtaagtcaag ttttggctta
1501 cagggaagag gctgtagcag gagcgtgcgt cgagggagaa gccgaaaccg gctttctcct
1561 cgtacgggcg accccacgat gacccacttc gcttgtaggc accttgatct atcgatgtga
1621 cacttaacgc cccccgtgaa tacggagagg ggtagtgcca ctgtttcgtt ttggccccag
1681 tcgagttaaa acgaccggga gtccagttcg aacgatattt taaagagaat gagttatctt
1741 cagtctcacc gtccgcgtaa acgcgaacgg aggggacgaa ggtctcgttc tccctatcaa
1801 gggtactaaa agctcgcaca ggtcaaacct cctaggaatg gaattccggc tacctacagc
1861 gatagccatg gtagcgtctc gctaaagaca ttaaaaatgg cattagctcg acaggaagtt
1921 gagcaggagg tacccagatc tgggctgtcc atgtgctggc gttcgaattt agcagcagcg
1981 gtttctttca taccagaacc gcgtggcacc agaccagaag aatgatgatg atgatggtgc
2041 atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg
2101 ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatcg atctcgatcc
2161 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
2221 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
2281 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
2341 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
2401 tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc
2461 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
2521 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
2581 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
2641 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
2701 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
2761 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
2821 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
2881 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
2941 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
3001 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
3061 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
3121 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
3181 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
3241 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
3301 gggctgcgcg ttggtgcgga catctcggta gtgggatacg acgataccga agacagctca
3361 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
3421 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
3481 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
3541 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
3601 tgagcgcaac gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat
3661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
3721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
3781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
3841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
3901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat
3961 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
4021 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
4081 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
4141 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
4201 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
4261 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
4321 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat
4381 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
4441 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
4501 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
4561 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
4621 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
4681 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
4741 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc
4801 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
4861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
4921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
4981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
5041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
5101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
5161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
5221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
5281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
5341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
5401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
5461 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
5521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
5581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
5641 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca
5701 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
5761 gaaacgtctt gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
5821 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
5881 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
5941 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
6001 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
6061 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
6121 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
6181 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
6241 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
6301 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
6361 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
6421 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
6481 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
6541 ctcgatgagt ttttctaaga attaattcat gagcggatac atatttgaat gtatttagaa
6601 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aaattgtaaa
6661 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
6721 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
6781 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
6841 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
6901 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
6961 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
7021 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
7081 gcttaatgcg ccgctacagg gcgcgtccca ttcgcca
//