LOCUS LT727562 4562 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pBRphoA, complete sequence.
ACCESSION LT727562
VERSION LT727562.1
KEYWORDS .
SOURCE Vector pBRphoA
ORGANISM Vector pBRphoA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4562)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4562
/organism="Vector pBRphoA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2954."
/db_xref="taxon:1945265"
regulatory 31..65
/note="tac"
/regulatory_class="promoter"
misc_feature 66..128
/note="5UTR"
misc_feature 129..191
/note="sphoA"
CDS 287..1477
/transl_table=11
/note="tetracyclin resistance"
/protein_id="SJL88847.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG
RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
rep_origin complement(2551..2953)
/note="pMB1 ori"
CDS complement(3494..4354)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88848.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1040 a 1263 c 1183 g 1076 t
ORIGIN
1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg
61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag
121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg
181 tgacaaaagc ggtacccggg gatcctctag agtcgacctg caggcatgca agctttaatg
241 cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa
301 tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat
361 gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta
421 tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc
481 actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat
541 cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt
601 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
661 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
721 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
781 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
841 taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
901 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
961 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
1021 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
1081 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
1141 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
1201 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
1261 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
1321 cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
1381 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
1441 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
1501 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
1561 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
1621 cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg
1681 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
1741 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
1801 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
1861 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
1921 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
1981 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
2041 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct
2101 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
2161 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
2221 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
2281 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
2341 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
2401 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
2461 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
2521 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
2581 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
2641 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
2701 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
2761 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
2821 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
2881 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
2941 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
3001 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
3061 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
3121 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
3181 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
3241 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
3301 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
3361 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3421 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
3481 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
3541 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
3601 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
3661 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
3721 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
3781 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
3841 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
3901 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
3961 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
4021 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
4081 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
4141 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
4201 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
4261 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
4321 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
4381 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
4441 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
4501 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
4561 aa
//