LOCUS LT727549 5422 bp DNA circular SYN 06-FEB-2017
DEFINITION Reporter vector pBLHIL6CAT, complete sequence.
ACCESSION LT727549
VERSION LT727549.1
KEYWORDS .
SOURCE Reporter vector pBLHIL6CAT
ORGANISM Reporter vector pBLHIL6CAT
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5422)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5422
/organism="Reporter vector pBLHIL6CAT"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2861."
/db_xref="taxon:1945201"
misc_feature 32..672
/note="5FR"
regulatory 673..1208
/note="hIL6"
/regulatory_class="promoter"
CDS 1260..1919
/transl_table=11
/note="chloramphenicol resistance"
/protein_id="SJL88812.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS <2009..2083
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88813.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature 2084..2150
/note="intron of SV40 small t-antigen"
misc_feature 2151..2702
/note="3' UTR of SV40 small t-antigen"
regulatory 2703..2799
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2703..2799)
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2938..3021)
/note="lac"
/regulatory_class="promoter"
rep_origin complement(3113..3515)
/note="pMB1 ori"
CDS complement(4056..4916)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88814.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5048..5122
/note="polyA"
/regulatory_class="terminator"
regulatory complement(5048..5122)
/note="polyA"
/regulatory_class="terminator"
regulatory 5263..5359
/note="polyA"
/regulatory_class="terminator"
regulatory complement(5263..5359)
/note="polyA"
/regulatory_class="terminator"
BASE COUNT 1503 a 1236 c 1203 g 1480 t
ORIGIN
1 aagcttgcat gcctgcaggt cgactctaga ggatcctcct gcaagagaca ccatcctgag
61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag
121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt
181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg
241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac
301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag
361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac
421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg
481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc
541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg
601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag
661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga
721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg
781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa
841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac
901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg
961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc
1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat
1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggat
1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc
1201 tgaggctcat tctgccctcg aggagcttgg cgagattttc aggagctaag gaagctaaaa
1261 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
1321 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
1381 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
1441 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
1501 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
1561 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
1621 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
1681 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
1741 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
1801 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
1861 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
1921 ttttttaagg cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa
1981 taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac ttctgtggtg
2041 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
2101 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
2161 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
2221 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
2281 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
2341 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
2401 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
2461 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
2521 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
2581 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
2641 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
2701 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
2761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
2821 ttgtccaaac tcatcaatgt atcttatcat gtctggatcg atccccgggt accgagctcg
2881 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
2941 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
3001 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
3061 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
3121 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
3181 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
3241 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
3301 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
3361 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
3421 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
3481 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
3541 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
3601 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
3661 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
3721 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
3781 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
3841 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
3901 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
3961 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
4021 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
4081 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
4141 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
4201 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
4261 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
4321 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
4381 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
4441 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
4501 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
4561 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
4621 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
4681 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
4741 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
4801 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
4861 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
4921 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
4981 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
5041 ccacctgaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
5101 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
5161 tgtatcttat catgtctgga tcataatcag ccataccaca tttgtagagg ttttacttgc
5221 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
5281 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
5341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
5401 atcttatcat gtctggatct cg
//