LOCUS       LT727518                4801 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Mammalian expression vector pCAGGS, complete sequence.
ACCESSION   LT727518
VERSION     LT727518.1
KEYWORDS    .
SOURCE      Mammalian expression vector pCAGGS
  ORGANISM  Mammalian expression vector pCAGGS
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Mammalian expression vector pCAGGS"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 2453."
                     /db_xref="taxon:1944753"
     regulatory      91..182
                     /note="3' UTR of rabbit beta-globin, incl. polyA"
                     /regulatory_class="terminator"
     misc_feature    183..539
                     /note="3' flanking region of rabbit beta-globin"
     regulatory      complement(617..699)
                     /note="lac promoter"
                     /regulatory_class="promoter"
     rep_origin      841..916
                     /note="SV40 ori"
     regulatory      947..1021
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     regulatory      complement(947..1021)
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     rep_origin      complement(1136..1537)
                     /note="pMB1 ori"
     CDS             complement(2078..2938)
                     /transl_table=11
                     /note="ampicillin resistance"
                     /protein_id="SJL88719.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      3074..3451
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     regulatory      3457..4798
                     /note="AG promoter"
                     /regulatory_class="promoter"
     misc_feature    3826..4750
                     /note="intron AG promoter"
BASE COUNT         1003 a         1272 c         1337 g         1189 t
ORIGIN      
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
      121 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
      181 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
      241 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
      301 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
      361 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
      421 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
      481 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
      541 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      601 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggt
      661 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      721 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
      781 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
      841 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
      901 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
      961 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1021 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     1081 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1141 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1201 gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca ggaaagaaca
     1261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1741 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2041 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2101 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2161 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2221 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2281 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2341 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2401 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2461 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2521 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2581 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2641 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2701 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2761 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2821 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2881 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2941 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3001 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3061 tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat tacggggtca
     3121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     3181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     3241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     3301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     3361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     3421 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
     3481 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     3541 ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg
     3601 ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg
     3661 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     3721 gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc
     3781 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
     3841 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg
     3901 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
     3961 gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc
     4021 ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg
     4081 agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc
     4141 gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa
     4201 ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc
     4261 cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg
     4321 ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc
     4381 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     4441 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     4501 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     4561 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     4621 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     4681 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
     4741 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     4801 a
//