LOCUS LT727510 4356 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCDM8, complete sequence.
ACCESSION LT727510
VERSION LT727510.1
KEYWORDS .
SOURCE Mammalian expression vector pCDM8
ORGANISM Mammalian expression vector pCDM8
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4356)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4356
/organism="Mammalian expression vector pCDM8"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2393."
/db_xref="taxon:1944910"
rep_origin complement(36..111)
/note="SV40 ori"
rep_origin complement(262..374)
/note="polyoma ori"
regulatory complement(1063..1159)
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory 1063..1159
/note="SV40 polyA late"
/regulatory_class="terminator"
misc_feature complement(1160..1558)
/note="3' UTR of SV40 small t-antigen"
misc_feature complement(1559..1625)
/note="intron of SV40 small t-antigen"
CDS complement(1626..>1700)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88695.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
regulatory complement(2171..2187)
/note="T7 promoter"
/regulatory_class="promoter"
regulatory complement(2232..2815)
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
misc_feature complement(2970..3172)
/note="supF"
rep_origin 3296..3754
/note="M13 ori"
rep_origin complement(3773..4005)
/note="pMB1 ori"
BASE COUNT 1085 a 1066 c 1056 g 1149 t
ORIGIN
1 gaattagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
61 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
121 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagta agacccgcac
181 ccgttcccaa actgcttatt aaagggggta tggaggtgct ggaccttgtg acagggccag
241 acagtgtgac agaaatagaa gcagtcctgt tttgacaagt tgcctctgga agcctctaca
301 atgcctctct tctttttctc cagagtaagc gggcccaggg gtccccggcc tctgcttaat
361 actaaaaaaa acagctgttg tcatagtaat gattgggtgg aaacattcta ggcctgggtg
421 gagaggcttt tgcttcctct tgcaaaacca cacgccctct ggagggcgtt gcctagcaac
481 taattaaaag aggatgtcgc acggccagct gcgtcagtta gtccacttcc tccttaactg
541 acttgacatt ttctatttta agagtcggga ggaaaattac tgtgttggag gccctcgccc
601 atcttctgaa gctgatcaag taagtgaatt ttcaaaatgg gagccgcact gactattcta
661 gtagacctca tcgagggatt agctgaagtg tctaccctta cgggactctc ggcagaagct
721 attttatctg gagaagccct cgctgccctt gatcgcgaaa ttacagctct cacttggtag
781 ggtgtaatga gttcggagac agccctagca actatgggta tttcagagga ggtgtatggg
841 tttgtcagta ctgtgcctgt gtttgtaagt cgaacagcgg gggctatatg gctgatgcag
901 acagttcaag gtgcctctac tatttcccta ggaatacagc ggtacctaca caacgaagag
961 gtccctactg taaatagaaa tatggcgttg ataccatggc gggatccaga catgataaga
1021 tacattgatg agtttggaca aaccacaact agaatgcagt gataaaaatg ctttatttgt
1081 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
1141 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
1201 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctctagtc aaggcactat
1261 acatcaaata tttttccata attttcttgt atagcagtgc agctttttcc tttgtggtgt
1321 aaatagcaaa gcaagcaaga gttctattac taaacacagc atgactcaaa aaacttacca
1381 attctgaagg aaagtccttg gggtcttcta cctttctctt cttttttgga ggagtagaat
1441 gttgagagtc agcagtagcc tcatcatcac tagatggcat ttcttctgag caaaacaggt
1501 tttcctcatt aaaggcattc caccactgct cccattcata agttccatag gttggaatct
1561 aaaataccac aacaattaga atcagtagtt taacacatta tacacttaaa aattttatat
1621 ttaccttaga gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt
1681 aaggttcctt cacaaagatc ctctagagtc gcggccgcga cctgcaggcg cagaactggt
1741 aggtatggaa gatccctcga gatccattgt gctggcggta ggcgagcagc gcctgcctga
1801 agctgcggga ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaagc
1861 gtatgattct ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg cttgttcctg
1921 aagtgccagt aaagcgccgg ctgctgcacc cccaaccgtt ccgccagttt gcgtgtcgtc
1981 agaccgtcta cgccgacctc gttcaacagt ccagggccgg cacggatcac tgtattcggc
2041 tgcaactttg tcatgcttga cactttatca ctgataaaca taatatgtcc accaacttat
2101 cagtgataaa gaatccgcgc cagcacaatg gatctcgagg tcgagggatc tctagaagct
2161 tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta
2221 gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
2281 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
2341 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
2401 ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg
2461 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
2521 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
2581 actgccaagt gggcagttta ccgtaaatag tccacccatt gacgtcaatg gaaagtccct
2641 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
2701 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta
2761 tgaactaatg accccgtaat tgattactat taataactag tcaataatca atgtcaacgc
2821 gtatatctgg cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag cagattcttc
2881 atgcaattgt cggtcaagcc ttgccttgtt gtagcttaaa ttttactcgc gcactactca
2941 gcgacctcca acacacaagc agggagcaga ttctttcgga cttttgaaag tgatggtggt
3001 gggggaagga ttcgaacctt cgaagtcgat gacggcagat ttagagtctg ctccctttgg
3061 ccgctcggga accccaccac gggtaatgct tttactggcc tgctccctta tcgggaagcg
3121 gggcgcatca tatcaaatga cgcgccgctg taaagtgtta cgttgagaaa gaccgcggta
3181 atccagctgg cgtaccgttc ctgtctaaaa tccctttaat cggcctcctg tttagctccc
3241 gctctgattc caacgaggaa agcacgttat acggtctcgt caaagcaacc atagtacgcg
3301 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
3361 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
3421 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
3481 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggcggcca
3541 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
3601 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
3661 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
3721 gcgaatttta acaaaatatt aacgtttaca atttctagct agaagctagc ttgcgttgct
3781 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
3841 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
3901 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
3961 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
4021 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
4081 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
4141 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
4201 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
4261 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
4321 cggtggtttt tttgtttgca agcagcagat tacgcc
//