LOCUS LT727495 6854 bp DNA circular SYN 06-FEB-2017
DEFINITION Vector pMaSVT, complete sequence.
ACCESSION LT727495
VERSION LT727495.1
KEYWORDS .
SOURCE Vector pMaSVT
ORGANISM Vector pMaSVT
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6854)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6854
/organism="Vector pMaSVT"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 2329."
/db_xref="taxon:1945341"
regulatory 24..105
/note="fdT"
/regulatory_class="terminator"
regulatory 134..215
/note="fdT"
/regulatory_class="terminator"
rep_origin 313..768
/note="f1 ori"
rep_origin complement(772..1145)
/note="pMB1 ori"
CDS complement(1686..2546)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88651.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature complement(2861..3520)
/note="chloramphenicol sensitive"
regulatory complement(3806..4101)
/note="SV40 late promoter"
/regulatory_class="promoter"
regulatory 3857..4138
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 4095..4170
/note="SV40 ori"
misc_feature 4209..4454
/note="SV40 large T-antigen"
CDS 4209..4733
/transl_table=11
/note="SV40 small t-antigen"
/protein_id="SJL88652.1"
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPD
KGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPE
CAKKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLW
CDIIGQTTYRDLKL"
misc_feature 4455..4800
/note="intron"
misc_feature 4801..6681
/note="SV40 large T-antigen"
regulatory 6682..6778
/note="polyA"
/regulatory_class="terminator"
regulatory complement(6682..6778)
/note="polyA"
/regulatory_class="terminator"
BASE COUNT 1953 a 1443 c 1550 g 1908 t
ORIGIN
1 aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt
61 ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc
121 gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag
181 attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg
241 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
301 cggatgcaga tcacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
721 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttga tctgcgctcg
781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
1021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
1081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
1141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
1201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
1261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
1321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
1381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
1441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
1501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
1561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
1621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
1681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
1741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
1801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
1861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
1921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
1981 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
2041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
2101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
2161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
2221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
2281 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
2341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
2401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
2461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
2521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcagacag
2581 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
2641 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg
2701 ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct
2761 cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
2821 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
2881 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg
2941 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
3001 ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg
3061 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
3121 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
3181 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
3241 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
3301 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
3361 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctaa acggtctggt
3421 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
3481 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
3541 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
3601 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
3661 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
3721 tttattcgaa gacgaaaggg catcgcgcgc ggggaattct catgtttgac agcttatcat
3781 cggcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
3841 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
3901 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
3961 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
4021 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
4081 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
4141 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
4201 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
4261 gtcttgaaag gagtgcctgg gggaatattc ctctgatgag aaaggcatat ttaaaaaaat
4321 gcaaggagtt tcatcctgat aaaggaggag atgaagaaaa aatgaagaaa atgaatactc
4381 tgtacaagaa aatggaagat ggagtaaaat atgctcatca acctgacttt ggaggcttct
4441 gggatgcaac tgaggtattt gcttcttcct taaatcctgg tgttgatgca atgtactgca
4501 aacaatggcc tgagtgtgca aagaaaatgt ctgctaactg catatgcttg ctgtgcttac
4561 tgaggatgaa gcatgaaaat agaaaattat acaggaaaga tccacttgtg tgggttgatt
4621 gctactgctt cgattgcttt agaatgtggt ttggacttga tctttgtgaa ggaaccttac
4681 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
4741 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
4801 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
4861 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
4921 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
4981 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
5041 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
5101 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
5161 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
5221 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tccattttct
5281 gttattgagg aaagtttgcc aggtgggtta aaggagcatg attttaatcc agaagaagca
5341 gaggaaacta aacaagtgtc ctggaagctt gtaacagagt atgcaatgga aacaaaatgt
5401 gatgatgtgt tgttattgct tgggatgtac ttggaatttc agtacagttt tgaaatgtgt
5461 ttaaaatgta ttaaaaaaga acagcccagc cactataagt accatgaaaa gcattatgca
5521 aatgctgcta tatttgctga cagcaaaaac caaaaaacca tatgccaaca ggctgttgat
5581 actgttttag ctaaaaagcg ggttgatagc ctacaattaa ctagagaaca aatgttaaca
5641 aacagattta atgatctttt ggataggatg gatataatgt ttggttctac aggctctgct
5701 gacatagaag aatggatggc tggagttgct tggctacact gtttgttgcc caaaatggat
5761 tcagtggtgt atgacttttt aaaatgcatg gtgtacaaca ttcctaaaaa aagatactgg
5821 ctgtttaaag gaccaattga tagtggtaaa actacattag cagctgcttt gcttgaatta
5881 tgtgggggga aagctttaaa tgttaatttg cccttggaca ggctgaactt tgagctagga
5941 gtagctattg accagttttt agtagttttt gaggatgtaa agggcactgg aggggagtcc
6001 agagatttgc cttcaggtca gggaattaat aacctggaca atttaaggga ttatttggat
6061 ggcagtgtta aggtaaactt agaaaagaaa cacctaaata aaagaactca aatatttccc
6121 cctggaatag tcaccatgaa tgagtacagt gtgcctaaaa cactgcaggc cagatttgta
6181 aaacaaatag attttaggcc caaagattat ttaaagcatt gcctggaacg cagtgagttt
6241 ttgttagaaa agagaataat tcaaagtggc attgctttgc ttcttatgtt aatttggtac
6301 agacctgtgg ctgagtttgc tcaaagtatt cagagcagaa ttgtggagtg gaaagagaga
6361 ttggacaaag agtttagttt gtcagtgtat caaaaaatga agtttaatgt ggctatggga
6421 attggagttt tagattggct aagaaacagt gatgatgatg atgaagacag ccaggaaaat
6481 gctgataaaa atgaagatgg tggggagaag aacatggaag actcagggca tgaaacaggc
6541 attgattcac agtcccaagg ctcatttcag gcccctcagt cctcacagtc tgttcatgat
6601 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
6661 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
6721 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
6781 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
6841 gctctagagg tcga
//