LOCUS LT727444 6213 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAMFhGNTIf1, complete sequence. ACCESSION LT727444 VERSION LT727444.1 KEYWORDS . SOURCE Mammalian expression vector pCAMFhGNTIf1 ORGANISM Mammalian expression vector pCAMFhGNTIf1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6213) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6213 /organism="Mammalian expression vector pCAMFhGNTIf1" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3124." /db_xref="taxon:1944901" regulatory 82..173 /note="polyA" /regulatory_class="terminator" misc_feature 174..530 /note="3FR" regulatory complement(608..690) /note="lac" /regulatory_class="promoter" rep_origin 832..907 /note="SV40 ori" regulatory 938..1012 /note="polyA" /regulatory_class="terminator" regulatory complement(938..1012) /note="polyA" /regulatory_class="terminator" rep_origin complement(1124..1528) /note="pMB1 ori" CDS complement(2069..2929) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88471.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3065..3442 /note="hCMV" /regulatory_class="promoter" regulatory 3448..4696 /note="AG" /regulatory_class="promoter" misc_feature 3817..4696 /note="intron" misc_feature 4707..4949 /note="ppMF" CDS <4962..6176 /codon_start=1 /transl_table=11 /note="hGNTI" /protein_id="SJL88472.1" /translation="ALDGDPASLTREVIRLAQDAEVELERQRGLLQQIGDALSSQRGR VPTAAPPAQPRVPVTPAPAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDC GHEETAQAIASYGSAVTHIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFR FPAAVVVEDDLEVAPDFFEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLY RTDFFPGLGWLLLAELWAELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRK GVSHGQFFDQHLKFIKLNQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRT NDRKELGEVRVQYTGRDSFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAP PLTWEGYDPSWN" BASE COUNT 1269 a 1696 c 1789 g 1459 t ORIGIN 1 gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc 61 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg 121 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg 181 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa 241 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg 301 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc 361 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta 421 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc 481 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg 541 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 601 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga 661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 721 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 781 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 841 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 901 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa 961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 1021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat 1081 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 1141 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 1201 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa 1261 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 1321 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 1381 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 1441 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 1501 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 1561 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 1621 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 1681 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 1741 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 1801 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 1861 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 1921 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 1981 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 2041 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 2101 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 2161 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 2221 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 2281 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 2341 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 2401 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 2461 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 2521 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 2581 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 2641 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 2701 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 2761 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 2821 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 2881 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 2941 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 3001 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 3061 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat 3121 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 3181 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 3241 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 3301 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 3361 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 3421 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc 3481 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 3541 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg 3601 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag 3661 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg 3721 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc 3781 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct 3841 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt 3901 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg 3961 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag 4021 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc 4081 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt 4141 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg 4201 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg 4261 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg 4321 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc 4381 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag 4441 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg 4501 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg 4561 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg 4621 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg 4681 tgtgaccggc ggctctagag tcgagaatga gatttccttc aatttttact gcagttttat 4741 tcgcagcatc ctccgcatta gctgctccag tcaacactac aacagaagat gaaacggcac 4801 aaattccggc tgaagctgtc atcggttact cagatttaga aggggatttc gatgttgctg 4861 ttttgccatt ttccaacagc acaaataacg ggttattgtt tataaatact actattgcca 4921 gcattgctgc taaagaagaa ggggtatctt tggataaaag ggctctcgat ggcgaccccg 4981 ccagcctcac ccgggaagtg attcgcctgg cccaagacgc cgaggtggag ctggagcggc 5041 agcgtgggct gctgcagcag atcggggatg ccctgtcgag ccagcggggg agggtgccca 5101 ccgcggcccc tcccgcccag ccgcgtgtgc ctgtgacccc cgcgccggcg gtgattccca 5161 tcctggtcat cgcctgtgac cgcagcactg ttcggcgctg cctggacaag ctgctgcatt 5221 atcggccctc ggctgagctc ttccccatca tcgttagcca ggactgcggg cacgaggaga 5281 cggcccaggc catcgcctcc tacggcagcg cggtcacgca catccggcag cccgacctga 5341 gcagcattgc ggtgccgccg gaccaccgca agttccaggg ctactacaag atcgcgcgcc 5401 actaccgctg ggcgctgggc caggtcttcc ggcagtttcg cttccccgcg gccgtggtgg 5461 tggaggatga cctggaggtg gccccggact tcttcgagta ctttcgggcc acctatccgc 5521 tgctgaaggc cgacccctcc ctgtggtgcg tctcggcctg gaatgacaac ggcaaggagc 5581 agatggtgga cgccagcagg cctgagctgc tctaccgcac cgactttttc cctggcctgg 5641 gctggctgct gttggccgag ctctgggctg agctggagcc caagtggcca aaggccttct 5701 gggacgactg gatgcggcgg ccggagcagc ggcaggggcg ggcctgcata cgccctgaga 5761 tctcaagaac gatgaccttt ggccgcaagg gtgtgagcca cgggcagttc tttgaccagc 5821 acctcaagtt tatcaagctg aaccagcagt ttgtgcactt cacccagctg gacctgtctt 5881 acctgcagcg ggaggcctat gaccgagatt tcctcgcccg cgtctacggt gctccccagc 5941 tgcaggtgga gaaagtgagg accaatgacc ggaaggagct gggggaggtg cgggtgcagt 6001 atacgggcag ggacagcttc aaggctttcg ccaaggctct gggtgtcatg gatgacctta 6061 agtcgggggt tccgagagct ggctaccggg gtattgtcac cttccagttc cggggccgcc 6121 gtgtccacct ggcgccccca ctgacgtggg agggctatga tcctagctgg aattagcacc 6181 tgtcgactgg agacctgcag gcatgcaagc tcg //