LOCUS LT727412 6167 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-hCASP-6, complete sequence.
ACCESSION LT727412
VERSION LT727412.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-hCASP-6
ORGANISM Mammalian expression vector pCAGGS-hCASP-6
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6167)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6167
/organism="Mammalian expression vector pCAGGS-hCASP-6"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3831."
/db_xref="taxon:1944871"
CDS 72..953
/transl_table=11
/note="hCASP6"
/protein_id="SJL88373.1"
/translation="MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRR
GIALIFNHERFFWHLTLPERRRTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKIHE
VSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFII
QACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTLPAGADFLMCYSVAEGYYS
HRETVNGSWYIQDLCEMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQVP
CFASMLTKKLHFFPKSN"
misc_feature 954..1403
/note="3UTR"
regulatory 1457..1548
/note="polyA"
/regulatory_class="terminator"
misc_feature 1549..1905
/note="3FR"
regulatory complement(1983..2065)
/note="lac"
/regulatory_class="promoter"
rep_origin 2207..2282
/note="SV40 ori"
regulatory 2313..2387
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2313..2387)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(2502..2903)
/note="pMB1 ori"
CDS complement(3444..4304)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88374.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 4440..4817
/note="hCMV"
regulatory 4823..6164
/note="AG"
/regulatory_class="promoter"
misc_feature 5192..6116
/note="intron"
BASE COUNT 1414 a 1565 c 1664 g 1524 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gtcgagctgc aatgagctcg gcctcggggc tccgcagggg gcacccggca ggtggggaag
121 aaaacatgac agaaacagat gccttctata aaagagaaat gtttgatccg gcagaaaagt
181 acaaaatgga ccacaggagg agaggaattg ctttaatctt caatcatgag aggttctttt
241 ggcacttaac actgccagaa aggcggcgca cctgcgcaga tagagacaat cttacccgca
301 ggttttcaga tctaggattt gaagtgaaat gctttaatga tcttaaagca gaagaactac
361 tgctcaaaat tcatgaggtg tcaactgtta gccacgcaga tgccgattgc tttgtgtgtg
421 tcttcctgag ccatggcgaa ggcaatcaca tttatgcata tgatgctaaa atcgaaattc
481 agacattaac tggcttgttc aaaggagaca agtgtcacag cctggttgga aaacccaaga
541 tatttatcat ccaggcatgt cggggaaacc agcacgatgt gccagtcatt cctttggatg
601 tagtagataa tcagacagag aagttggaca ccaacataac tgaggtggat gcagcctccg
661 tttacacgct gcctgctgga gctgacttcc tcatgtgtta ctctgttgca gaaggatatt
721 attctcaccg ggaaactgtg aacggctcat ggtacattca agatttgtgt gagatgttgg
781 gaaaatatgg ctcctcctta gagttcacag aactcctcac actggtgaac aggaaagttt
841 ctcagcgccg agtggacttt tgcaaagacc caagtgcaat tggaaagaag caggttccct
901 gttttgcctc aatgctaact aaaaagctgc atttctttcc aaaatctaat taattaatag
961 aggctatcta atttcacact ctgtattgaa aatggctttc tcagccaggc gtggttactc
1021 acacctgtaa tcccagcact ttgggagtcc aaggtgggcg gatcacctga ggtcgggagt
1081 tcgagaccag cctgaccaac atggcagaag ccccgcctct actaaaaatg caaaaaaaaa
1141 tttagctagg catggcggcg catgcctgca atcccagcta cttggaaggc tgaggcagga
1201 gaatcacttg aacccaggag gtggaggctg cggtgagccg agcattgcgc cattgcactc
1261 cagcctgggc aacgagtgaa actccgtctc aaaaaaaaag aaaatgtctt tctcttcctt
1321 ttatataaat atcgttaggg tgaagcatta tggtctaatg attcaaatgt tttaaagttt
1381 aatgcctagc agagaactgc cttaaaaaaa aaaaaaaaaa aactcgacca atgccctggc
1441 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
1501 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
1561 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
1621 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
1681 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
1741 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
1801 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
1861 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
1921 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
1981 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag
2041 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
2101 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
2161 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
2221 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
2281 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
2401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
2461 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
2521 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
2581 ggtaatacgg ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc
2641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
2701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
2761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
2821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
2881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
2941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
3001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
3061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
3121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
3181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
3241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
3301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
3361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
3421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
3481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
3541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
3601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
3661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
3721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
3781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
3841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
3901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
3961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
4021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
4081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
4141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
4201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
4261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
4321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
4381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg
4441 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
4501 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
4561 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
4621 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
4681 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
4741 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
4801 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
4861 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
4921 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg
4981 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
5041 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
5101 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
5161 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
5221 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
5281 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
5341 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
5401 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
5461 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
5521 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
5581 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
5641 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
5701 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg
5761 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
5821 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
5881 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
5941 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
6001 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
6061 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
6121 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa
//