LOCUS LT727410 5815 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGSp35, complete sequence. ACCESSION LT727410 VERSION LT727410.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGSp35 ORGANISM Mammalian expression vector pCAGGSp35 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5815) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5815 /organism="Mammalian expression vector pCAGGSp35" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3829." /db_xref="taxon:1944891" misc_feature 4..21 /note="5UTR" CDS 22..924 /transl_table=11 /note="p35" /protein_id="SJL88369.1" /translation="MMCVIFPVEIDVSQTIIRDCQVDKQTRELVYINKIMNTQLTKPV LMMFNISGPIRSVTRKNNNLRDRIKSKVDEQFDQLERDYSDQMDGFHDSIKYFKDEHY SVSCQNGSVLKSKFAKILKSHDYTDKKSIEAYEKYCLPKLVDERNDYYVAVCVLKPGF ENGSNQVLSFEYNPIGNKVIVPFAHEINDTGLYEYDVVAYVDSVQFDGEQFEEFVQSL ILPSSFKNSEKVLYYNEASKNKSMIYKALEFTTESSWGKSEKYNWKIFCNGFIYDKKS KVLYVKLHNVTSALNKNVILNTIK" misc_feature 925..1023 /note="3UTR" regulatory 1105..1196 /note="polyA" /regulatory_class="terminator" misc_feature 1197..1553 /note="3FR" regulatory complement(1631..1713) /note="lac" /regulatory_class="promoter" rep_origin 1855..1930 /note="SV40 ori" regulatory 1961..2035 /note="polyA" /regulatory_class="terminator" regulatory complement(1961..2035) /note="polyA" /regulatory_class="terminator" rep_origin complement(2150..2551) /note="pMB1 ori" CDS complement(3092..3952) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88370.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" misc_feature 4088..4465 /note="hCMV" regulatory 4471..5812 /note="AG" /regulatory_class="promoter" misc_feature 4840..5764 /note="intron" BASE COUNT 1350 a 1434 c 1546 g 1485 t ORIGIN 1 ttcgatcttt accatagcaa aatgatgtgt gtaatttttc cggtagaaat cgacgtgtcc 61 cagacgatta ttcgagattg tcaggtggac aaacaaacca gagagttggt gtacattaac 121 aagattatga acacgcaatt gacaaaaccc gttctcatga tgtttaacat ttcgggtcct 181 atacgaagcg ttacgcgcaa gaacaacaat ttgcgcgaca gaataaaatc aaaagtcgat 241 gaacaatttg atcaactaga acgcgattac agcgatcaaa tggatggatt ccacgatagc 301 atcaagtatt ttaaagatga acactattcg gtaagttgcc aaaatggcag cgtgttgaaa 361 agcaagtttg ctaaaatttt aaagagtcat gattataccg ataaaaagtc tattgaagct 421 tacgagaaat actgtttgcc caaattggtc gacgaacgca acgactacta cgtggcggta 481 tgcgtgttga agccgggatt tgagaacggc agcaaccaag tgctatcttt cgagtacaac 541 ccgattggta acaaagttat tgtgccgttt gctcacgaaa ttaacgacac gggactttac 601 gagtacgacg tcgtagctta cgtggacagt gtgcagtttg atggcgaaca atttgaagag 661 tttgtgcaga gtttaatatt gccgtcgtcg ttcaaaaatt cggaaaaggt tttatattac 721 aacgaagcgt cgaaaaacaa aagcatgatc tacaaggctt tagagtttac tacagaatcg 781 agctggggca aatccgaaaa gtataattgg aaaatttttt gtaacggttt tatttatgat 841 aaaaaatcaa aagtgttgta tgttaaattg cacaatgtaa ctagtgcact caacaaaaat 901 gtaatattaa acacaattaa ataaatgtta aaatttattg cctaatatta ttttgtcatt 961 gcttgtcatt tattaatttg gatgatgtca tttgttttta aaattgaact ggctttacga 1021 gtagaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat 1081 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc 1141 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata 1201 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta 1261 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc 1321 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat 1381 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg 1441 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc 1501 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac 1561 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 1621 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct gggtgcctaa 1681 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 1741 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc 1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 1921 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta 1981 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 2041 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg 2101 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 2161 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 2221 tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag 2281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 2341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 2401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 2461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 2521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 2581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 2641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 2701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 2761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 2821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 2881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 2941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 3001 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 3061 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 3121 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 3181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 3241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 3301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 3361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 3421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 3481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 3541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 3601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 3661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 3721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 3781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 3841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 3901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 3961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 4021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 4081 ctgggtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt 4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca 4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca 4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 4441 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc 4501 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt 4561 gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg gcggggcgag 4621 gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga 4681 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg 4741 cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc 4801 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct 4861 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg 4921 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt 4981 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt 5041 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg 5101 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg 5161 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc 5221 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg 5281 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc 5341 ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg gcccccggag 5401 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga 5461 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg 5521 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg 5581 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg 5641 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg 5701 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct 5761 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaa //