LOCUS LT727400 6282 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-mCASP-11a, complete sequence.
ACCESSION LT727400
VERSION LT727400.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-mCASP-11a
ORGANISM Mammalian expression vector pCAGGS-mCASP-11a
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6282)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6282
/organism="Mammalian expression vector pCAGGS-mCASP-11a"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3817."
/db_xref="taxon:1944876"
misc_feature complement(182..376)
/note="3UTR"
CDS complement(377..1498)
/transl_table=11
/note="CASP11"
/protein_id="SJL88349.1"
/translation="MAENKHPDKPLKVLEQLGKEVLTEYLEKLVQSNVLKLKEEDKQK
FNNAERSDKRWVFVDAMKKKHSKVGEMLLQTFFSVDPGSHHGEANLEMEEPEESLNTL
KLCSPEEFTRLCREKTQEIYPIKEANGRTRKALIICNTEFKHLSLRYGANFDIIGMKG
LLEDLGYDVVVKEELTAEGMESEMKDFAALSEHQTSDSTFLVLMSHGTLHGICGTMHS
EKTPDVLQYDTIYQIFNNCHCPGLRDKPKVIIVQACRGGNSGEMWIRESSKPQLCRGV
DLPRNMEADAVKLSHVEKDFIAFYSTTPHHLSYRDKTGGSYFITRLISCFRKHACSCH
LFDIFLKVQQSFEKASIHSQMPTIDRATLTRYFYLFPGN"
misc_feature complement(1499..1524)
/note="5UTR"
regulatory 1572..1663
/note="polyA"
/regulatory_class="terminator"
misc_feature 1664..2020
/note="3FR"
regulatory complement(2098..2180)
/note="lac"
/regulatory_class="promoter"
rep_origin 2322..2397
/note="SV40 ori"
regulatory 2428..2507
/note="polyA"
/regulatory_class="terminator"
regulatory complement(2428..2502)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(2617..3018)
/note="pMB1 ori"
CDS complement(3559..4419)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88350.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 4555..4932
/note="hCMV"
regulatory 4938..6279
/note="AG"
/regulatory_class="promoter"
misc_feature 5307..6231
/note="intron"
BASE COUNT 1363 a 1594 c 1655 g 1670 t
ORIGIN
1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
61 gcgcgtttgg aatcactaca gggatgttta ataccactac aatggatgat gtatataact
121 atctattcga tgatgaagat accccaccaa acccaaaaaa agagatcgaa ttcggcacga
181 gttttttttt ttttttgggc ttgtttgctt gtctatggaa cagaatccca ctgtgttgct
241 taggctggtc ttgaattcat aagtattctt tccttaattt cactagttct ggaatttcag
301 gaatagaatg tgatccttgg ccaagatcac ttcttcaagc tgaagaaatg agattcagtt
361 gcttgttgct ttgttctcag ttgccaggaa agaggtagaa atatctcgtc aaggttgccc
421 gatcaatggt gggcatctgg gaatgaatac ttgccttttc aaatgattgt tgcaccttca
481 ggaatatatc aaagagatga caagagcaag catgtttccg gaagcaggaa atgagtctag
541 tgatgaagta agagcctcct gttttgtctc ggtaggacaa gtgatgtggg gttgtagagt
601 agaaggcaat gaagtccttc tccacgtggc tcagcttgac agcatcagct tccatattcc
661 taggtagatc tacacctctg cacaactggg gttttgaaga ctctctgatc cacatttctc
721 cagagttccc acctctgcag gcctgcacaa tgatgacttt gggtttgtct cgtagacctg
781 gacagtggca attgttgaat atctgataga tggtatcata ctgtagcaca tctggagttt
841 tttcactgtg cattgttcca caaatgccat gcagtgtgcc atgagacatt agcaccagga
901 atgtgctgtc tgatgtctgg tgttctgaga gtgcagcaaa gtctttcatc tctgactcca
961 tgccctctgc tgtaagctcc tctttcacca ccacatcgta gcctaagtct tcaagaaggc
1021 ctttcatacc aatgatgtca aagttagccc catacctcag tgagagatgt ttgaactctg
1081 tattgcatat gataagagcc tttcgtgtac ggccattggc ctcctttatt gggtaaattt
1141 cttgtgtctt ttctctgcaa agccttgtga actcttcagg ggaacaaagc ttgagagtgt
1201 tcaatgattc ttctggttcc tccatttcca gattagcttc accatggtgg ctgcctgggt
1261 ccacactgaa gaatgtctgg agaagcattt cacctacttt gctgtgtttc tttttcatgg
1321 catctacaaa aacccaacgc ttgtcactgc gttcagcatt gttaaatttt tgtttatctt
1381 cctcctttaa tttcagtaca ttgctttgta ctaatttttc taggtactcc gtaaggactt
1441 ctttgcccag ctgttccaac accttaagtg gtttgtcagg gtgtttgttt tcagccatga
1501 gaaaaagcct cagttctttc gcaccgctcg tgccgaattc ccccaatgcc ctggctcaca
1561 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
1621 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
1681 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
1741 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
1801 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
1861 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
1921 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
1981 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
2041 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
2101 cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac
2161 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
2221 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
2281 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
2341 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
2401 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
2461 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
2521 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
2581 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
2641 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
2701 tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca
2761 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
2821 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
2881 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
2941 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
3001 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
3061 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
3121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
3181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
3241 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
3301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
3361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
3421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
3481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
3541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
3601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
3661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
3721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
3781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
3841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
3901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
3961 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
4021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
4081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
4141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
4201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
4261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
4321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
4381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
4441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
4501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt
4561 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
4621 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
4681 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
4741 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
4801 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
4861 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
4921 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
4981 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
5041 cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg
5101 cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
5161 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
5221 ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
5281 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
5341 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
5401 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
5461 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
5521 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
5581 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
5641 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
5701 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
5761 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
5821 ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt
5881 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
5941 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
6001 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
6061 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
6121 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
6181 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
6241 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa
//