LOCUS       LT727399                6280 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Mammalian expression vector pCAGGS-mCASP-11, complete sequence.
ACCESSION   LT727399
VERSION     LT727399.1
KEYWORDS    .
SOURCE      Mammalian expression vector pCAGGS-mCASP-11
  ORGANISM  Mammalian expression vector pCAGGS-mCASP-11
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Mammalian expression vector pCAGGS-mCASP-11"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 3816."
                     /db_xref="taxon:1944875"
     misc_feature    80..105
                     /note="5' UTR of mCASP-11"
     CDS             106..1227
                     /transl_table=11
                     /note="mCASP-11"
                     /protein_id="SJL88347.1"
                     /translation="MAENKHPDKPLKVLEQLGKEVLTEYLEKLVQSNVLKLKEEDKQK
                     FNNAERSDKRWVFVDAMKKKHSKVGEMLLQTFFSVDPGSHHGEANLEMEEPEESLNTL
                     KLCSPEEFTRLCREKTQEIYPIKEANGRTRKALIICNTEFKHLSLRYGANFDIIGMKG
                     LLEDLGYDVVVKEELTAEGMESEMKDFAALSEHQTSDSTFLVLMSHGTLHGICGTMHS
                     EKTPDVLQYDTIYQIFNNCHCPGLRDKPKVIIVQACRGGNSGEMWIRESSKPQLCRGV
                     DLPRNMEADAVKLSHVEKDFIAFYSTTPHHLSYRDKTGGSYFITRLISCFRKHACSCH
                     LFDIFLKVQQSFEKASIHSQMPTIDRATLTRYFYLFPGN"
     misc_feature    1228..1420
                     /note="3' UTR of mCASP-11"
     regulatory      1570..1661
                     /note="beta-globin polyA"
                     /regulatory_class="terminator"
     misc_feature    1662..2018
                     /note="3' FR of beta-globin"
     regulatory      complement(2096..2178)
                     /note="lac promoter"
                     /regulatory_class="promoter"
     rep_origin      2320..2395
                     /note="SV40 ori"
     regulatory      complement(2426..2500)
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      2426..2500
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     rep_origin      complement(2615..3016)
                     /note="pMB1 ori"
     CDS             complement(3557..4417)
                     /transl_table=11
                     /note="ampicillin resistance"
                     /protein_id="SJL88348.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      4553..4930
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     regulatory      4936..6277
                     /note="AG promoter"
                     /regulatory_class="promoter"
     misc_feature    5305..6229
                     /note="intron AG promoter"
BASE COUNT         1483 a         1588 c         1659 g         1550 t
ORIGIN      
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 ggggaattcg gcacgagcgg tgcgaaagaa ctgaggcttt ttctcatggc tgaaaacaaa
      121 caccctgaca aaccacttaa ggtgttggaa cagctgggca aagaagtcct tacggagtac
      181 ctagaaaaat tagtacaaag caatgtactg aaattaaagg aggaagataa acaaaaattt
      241 aacaatgctg aacgcagtga caagcgttgg gtttttgtag atgccatgaa aaagaaacac
      301 agcaaagtag gtgaaatgct tctccagaca ttcttcagtg tggacccagg cagccaccat
      361 ggtgaagcta atctggaaat ggaggaacca gaagaatcat tgaacactct caagctttgt
      421 tcccctgaag agttcacaag gctttgcaga gaaaagacac aagaaattta cccaataaag
      481 gaggccaatg gccgtacacg aaaggctctt atcatatgca atacagagtt caaacatctc
      541 tcactgaggt atggggctaa ctttgacatc attggtatga aaggccttct tgaagactta
      601 ggctacgatg tggtggtgaa agaggagctt acagcagagg gcatggagtc agagatgaaa
      661 gactttgctg cactctcaga acaccagaca tcagacagca cattcctggt gctaatgtct
      721 catggcacac tgcatggcat ttgtggaaca atgcacagtg aaaaaactcc agatgtgcta
      781 cagtatgata ccatctatca gatattcaac aattgccact gtccaggtct acgagacaaa
      841 cccaaagtca tcattgtgca ggcctgcaga ggtgggaact ctggagaaat gtggatcaga
      901 gagtcttcaa aaccccagtt gtgcagaggt gtagatctac ctaggaatat ggaagctgat
      961 gctgtcaagc tgagccacgt ggagaaggac ttcattgcct tctactctac aaccccacat
     1021 cacttgtcct accgagacaa aacaggaggc tcttacttca tcactagact catttcctgc
     1081 ttccggaaac atgcttgctc ttgtcatctc tttgatatat tcctgaaggt gcaacaatca
     1141 tttgaaaagg caagtattca ttcccagatg cccaccattg atcgggcaac cttgacgaga
     1201 tatttctacc tctttcctgg caactgagaa caaagcaaca agcaactgaa tctcatttct
     1261 tcagcttgaa gaagtgatct tggccaagga tcacattcta ttcctgaaat tccagaacta
     1321 gtgaaattaa ggaaagaata cttatgaatt caagaccagc ctaagcaaca cagtgggatt
     1381 ctgttccata gacaagcaaa caagcaaaaa taaaaaaaaa ctcgtgccga attcgatctc
     1441 tttttttggg tttggtgggg tatcttcatc atcgaataga tagttatata catcatccat
     1501 tgtagtggta ttaaacatcc ctgtagtgat tccaaacgcg ccaatgccct ggctcacaaa
     1561 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     1621 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     1681 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     1741 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     1801 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     1861 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     1921 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     1981 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     2041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2101 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
     2161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     2221 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     2281 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2341 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2401 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     2461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2521 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     2581 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2701 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2761 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2821 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2881 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2941 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3001 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3061 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3121 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3181 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3241 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3301 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3361 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3541 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3721 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3781 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3841 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3961 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4021 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4081 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4141 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4261 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4321 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4381 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4441 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4501 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga
     4561 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4621 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4681 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4741 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4801 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4861 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4921 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     4981 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     5041 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg
     5101 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     5161 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt
     5221 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
     5281 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     5341 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
     5401 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     5461 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     5521 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     5581 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     5641 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     5701 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     5761 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     5821 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg
     5881 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact
     5941 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag
     6001 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg
     6061 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga
     6121 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg
     6181 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa
     6241 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//