LOCUS LT727399 6280 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-mCASP-11, complete sequence. ACCESSION LT727399 VERSION LT727399.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-mCASP-11 ORGANISM Mammalian expression vector pCAGGS-mCASP-11 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6280) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6280 /organism="Mammalian expression vector pCAGGS-mCASP-11" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 3816." /db_xref="taxon:1944875" misc_feature 80..105 /note="5' UTR of mCASP-11" CDS 106..1227 /transl_table=11 /note="mCASP-11" /protein_id="SJL88347.1" /translation="MAENKHPDKPLKVLEQLGKEVLTEYLEKLVQSNVLKLKEEDKQK FNNAERSDKRWVFVDAMKKKHSKVGEMLLQTFFSVDPGSHHGEANLEMEEPEESLNTL KLCSPEEFTRLCREKTQEIYPIKEANGRTRKALIICNTEFKHLSLRYGANFDIIGMKG LLEDLGYDVVVKEELTAEGMESEMKDFAALSEHQTSDSTFLVLMSHGTLHGICGTMHS EKTPDVLQYDTIYQIFNNCHCPGLRDKPKVIIVQACRGGNSGEMWIRESSKPQLCRGV DLPRNMEADAVKLSHVEKDFIAFYSTTPHHLSYRDKTGGSYFITRLISCFRKHACSCH LFDIFLKVQQSFEKASIHSQMPTIDRATLTRYFYLFPGN" misc_feature 1228..1420 /note="3' UTR of mCASP-11" regulatory 1570..1661 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 1662..2018 /note="3' FR of beta-globin" regulatory complement(2096..2178) /note="lac promoter" /regulatory_class="promoter" rep_origin 2320..2395 /note="SV40 ori" regulatory complement(2426..2500) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 2426..2500 /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(2615..3016) /note="pMB1 ori" CDS complement(3557..4417) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL88348.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 4553..4930 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4936..6277 /note="AG promoter" /regulatory_class="promoter" misc_feature 5305..6229 /note="intron AG promoter" BASE COUNT 1483 a 1588 c 1659 g 1550 t ORIGIN 1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 61 ggggaattcg gcacgagcgg tgcgaaagaa ctgaggcttt ttctcatggc tgaaaacaaa 121 caccctgaca aaccacttaa ggtgttggaa cagctgggca aagaagtcct tacggagtac 181 ctagaaaaat tagtacaaag caatgtactg aaattaaagg aggaagataa acaaaaattt 241 aacaatgctg aacgcagtga caagcgttgg gtttttgtag atgccatgaa aaagaaacac 301 agcaaagtag gtgaaatgct tctccagaca ttcttcagtg tggacccagg cagccaccat 361 ggtgaagcta atctggaaat ggaggaacca gaagaatcat tgaacactct caagctttgt 421 tcccctgaag agttcacaag gctttgcaga gaaaagacac aagaaattta cccaataaag 481 gaggccaatg gccgtacacg aaaggctctt atcatatgca atacagagtt caaacatctc 541 tcactgaggt atggggctaa ctttgacatc attggtatga aaggccttct tgaagactta 601 ggctacgatg tggtggtgaa agaggagctt acagcagagg gcatggagtc agagatgaaa 661 gactttgctg cactctcaga acaccagaca tcagacagca cattcctggt gctaatgtct 721 catggcacac tgcatggcat ttgtggaaca atgcacagtg aaaaaactcc agatgtgcta 781 cagtatgata ccatctatca gatattcaac aattgccact gtccaggtct acgagacaaa 841 cccaaagtca tcattgtgca ggcctgcaga ggtgggaact ctggagaaat gtggatcaga 901 gagtcttcaa aaccccagtt gtgcagaggt gtagatctac ctaggaatat ggaagctgat 961 gctgtcaagc tgagccacgt ggagaaggac ttcattgcct tctactctac aaccccacat 1021 cacttgtcct accgagacaa aacaggaggc tcttacttca tcactagact catttcctgc 1081 ttccggaaac atgcttgctc ttgtcatctc tttgatatat tcctgaaggt gcaacaatca 1141 tttgaaaagg caagtattca ttcccagatg cccaccattg atcgggcaac cttgacgaga 1201 tatttctacc tctttcctgg caactgagaa caaagcaaca agcaactgaa tctcatttct 1261 tcagcttgaa gaagtgatct tggccaagga tcacattcta ttcctgaaat tccagaacta 1321 gtgaaattaa ggaaagaata cttatgaatt caagaccagc ctaagcaaca cagtgggatt 1381 ctgttccata gacaagcaaa caagcaaaaa taaaaaaaaa ctcgtgccga attcgatctc 1441 tttttttggg tttggtgggg tatcttcatc atcgaataga tagttatata catcatccat 1501 tgtagtggta ttaaacatcc ctgtagtgat tccaaacgcg ccaatgccct ggctcacaaa 1561 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag 1621 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt 1681 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag 1741 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg 1801 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga 1861 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta 1921 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta 1981 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt 2041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 2101 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc 2161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg 2221 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 2281 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 2341 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 2401 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata 2461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 2521 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc 2581 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 2641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 2701 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 2761 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 2821 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 2881 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 2941 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 3001 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 3061 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 3121 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 3181 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 3241 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 3301 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 3361 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 3421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 3481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 3541 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 3601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 3661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 3721 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 3781 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 3841 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 3901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 3961 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 4021 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 4081 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 4141 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 4201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 4261 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 4321 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 4381 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 4441 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 4501 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga 4561 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 4621 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 4681 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 4741 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 4801 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 4861 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 4921 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc 4981 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg 5041 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg 5101 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg 5161 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt 5221 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc 5281 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt 5341 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag 5401 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg 5461 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc 5521 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc 5581 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg 5641 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc 5701 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg 5761 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct 5821 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg 5881 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact 5941 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag 6001 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 6061 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga 6121 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 6181 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 6241 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa //