LOCUS LT727386 6560 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-TAX1BP1-S, complete sequence.
ACCESSION LT727386
VERSION LT727386.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-TAX1BP1-S
ORGANISM Mammalian expression vector pCAGGS-TAX1BP1-S
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6560)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6560
/organism="Mammalian expression vector pCAGGS-TAX1BP1-S"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3780."
/db_xref="taxon:1944898"
CDS 19..1710
/transl_table=11
/note="hTAX1BP1-S"
/protein_id="SJL88313.1"
/translation="MERELNHEKERCDQLQAEQKGLTEVTQSLKMENEEFKKRFSDAT
SKAHQLEEDIVSVTHKAIEKETELDSLKDKLKKAQHEREQLECQLKTEKDEKELYKVH
LKNTEIENTKLMSEVQTLKNLDGNKESVITHFKEEIGRLQLCLAEKENLQRTFLLTTS
SKEDTCFLKEQLRKAEEQVQATRQEVVFLAKELSDAVNVRDRTMADLHTARLENEKVK
KQLADAVAELKLNAMKKDQDKTDTLEHELRREVEDLKLRLQMAADHYKEKFKECQRLQ
KQINKLSDQSANNNNVFTKKTGNQQKVNDASVNTDPATSASTVDVKPSPSAAEADFDI
VTKGQVCEMTKEIADKTEKYNKCKQLLQDEKAKCNKYADELAKMELKWKEQVKIAENV
KLELAEVQDNYKELKRSLENPAERKMEDGADGAFYPDEIQRPPVRVPSWGLEDNVVCS
QPARNFSRPDGLEDSEDSKEDENVPTAPDPPSQHLRGHGTGFCFDSSFDVHKKCPLCE
LMFPPNYDQSKFEEHVESHWKVCPMCSEQFPPDYDQQVFERHVQTHFDQNVLNFD"
regulatory 1852..1943
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1944..2300
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2378..2460)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2602..2677
/note="SV40 ori"
regulatory 2708..2782
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2708..2782)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2897..3298)
/note="pMB1 ori"
CDS complement(3839..4699)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88314.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4835..5212
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5218..6559
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5587..6511
/note="intron AG promoter"
BASE COUNT 1677 a 1569 c 1730 g 1584 t
ORIGIN
1 aattcggcac gagggagaat ggaaagagaa cttaaccatg agaaagaaag atgtgaccaa
61 ctgcaagcag aacaaaaggg tcttactgaa gtaacacaaa gcttaaaaat ggaaaatgaa
121 gagtttaaga agaggttcag tgatgctaca tccaaagccc atcagcttga ggaagatatt
181 gtgtcagtaa cacataaagc aattgaaaaa gaaaccgaat tagacagttt aaaggacaaa
241 ctcaagaagg cacaacatga aagagaacaa cttgaatgtc agttgaagac agagaaggat
301 gaaaaggaac tttataaggt acatttgaag aatacagaaa tagaaaatac caagcttatg
361 tcagaggtcc agactttaaa aaatttagat gggaacaaag aaagcgtgat tactcatttc
421 aaagaagaga ttggcaggct gcagttatgt ttggctgaaa aggaaaatct gcaaagaact
481 ttcctgctta caacctcaag taaagaagat acttgttttt taaaggagca acttcgtaaa
541 gcagaggaac aggttcaggc aactcggcaa gaagttgtct ttctggctaa agaactcagt
601 gatgctgtca acgtacgaga cagaacgatg gcagacctgc atactgcacg cttggaaaac
661 gagaaagtga aaaagcagtt agctgatgca gtggcagaac ttaaactaaa tgctatgaaa
721 aaagatcagg acaagactga tacactggaa cacgaactaa gaagagaagt tgaagatctg
781 aaactccgtc ttcagatggc tgcagaccat tataaagaaa aatttaagga atgccaaagg
841 ctccaaaaac aaataaacaa actttcagat caatcagcta ataataataa tgtcttcaca
901 aagaaaacgg ggaatcagca gaaagtgaat gatgcttcag taaacacaga cccagccact
961 tctgcctcta ctgtagatgt aaagccatca ccttctgcag cagaggcaga ttttgacata
1021 gtaacaaagg ggcaagtctg tgaaatgacc aaagaaattg ctgacaaaac agaaaagtat
1081 aataaatgta aacaactctt gcaggatgag aaagcaaaat gcaataaata tgctgatgaa
1141 cttgcaaaaa tggagctgaa atggaaagaa caagtgaaaa ttgctgaaaa tgtaaaactt
1201 gaactagctg aagtacagga caattataaa gaacttaaaa ggagtctaga aaatccagca
1261 gaaaggaaaa tggaagatgg agcagatggt gctttttacc cagatgaaat acaaaggcca
1321 cctgtcagag tcccctcttg gggactggaa gacaatgttg tctgcagcca gcctgctcga
1381 aactttagtc ggcctgatgg cttagaggac tctgaggata gcaaagaaga tgagaatgtg
1441 cctactgctc ctgatcctcc aagtcaacat ttacgtgggc atgggacagg cttttgcttt
1501 gattccagct ttgatgttca caagaagtgt cccctctgtg agttaatgtt tcctcctaac
1561 tatgatcaga gcaaatttga agaacatgtt gaaagtcact ggaaggtgtg cccgatgtgc
1621 agcgagcagt tccctcctga ctatgaccag caggtgtttg aaaggcatgt gcagacccat
1681 tttgatcaga atgttctaaa ttttgactag ttacttttta ttatgagtta atatagttta
1741 gcagtaaaaa aaaaaaaaaa aaaactcgag gaattcactc ctcaggtgca ggctgcctat
1801 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
1861 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
1921 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
1981 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
2041 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
2101 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
2161 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
2221 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
2281 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
2341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
2401 cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
2461 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
2521 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
2581 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
2641 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
2701 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
2761 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
2821 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
2881 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
2941 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
3001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
3061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
3121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
3181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
3241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
3301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
3361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
3421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
3481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
3541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
3601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
3661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
3721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
3781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
3841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
3901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
3961 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
4021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
4081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
4141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
4201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
4261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
4321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
4381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
4441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
4501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
4561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
4621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
4681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
4741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
4801 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa
4861 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
4921 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
4981 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
5041 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
5101 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
5161 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
5221 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
5281 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc
5341 cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg
5401 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
5461 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc
5521 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
5581 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
5641 tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct
5701 ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
5761 tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
5821 ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc
5881 tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg
5941 ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc
6001 ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg
6061 gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg
6121 ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
6181 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
6241 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
6301 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
6361 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
6421 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
6481 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
6541 gtctcatcat tttggcaaag
//